PMID- 29804411
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20211204
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 98
IP  - 18
DP  - 2018 May 15
TI  - [The expression of Akt/mTOR in VSMC calcification induced by high phosphate and 
      its regulation of Cbfalpha1].
PG  - 1446-1451
LID - 10.3760/cma.j.issn.0376-2491.2018.18.016 [doi]
AB  - Objective: To observe the expression of protein kinase B (Akt) / mammalian target 
      of rapamycin (mTOR) induced by high phosphorus in rat vascular smooth muscle 
      cells (VSMC) calcification model, and its modulation on the expression of core 
      binding factor alpha 1 (Cbfalpha1). Methods: Rat VSMC cells were cultured in vitro, 
      and then divided into two groups: normal phosphorus group (Pi 1.3 mmol/L) and 
      high phosphorus group (Pi 2.6 mmol/L). At day 7, calcium deposition was detected 
      by Alizarin stain. The mRNA levels of Cbfalpha1 and osteopontin (OPN) were determined 
      by real-time PCR. The protein expressions of p-Akt (ser473), p-mTOR (S2448), 
      Cbfalpha1 and OPN were quantified by Western blot. Then, VSMC cultured with high 
      phosphorus were treated with Akt inhibitor (Wortmannin) and mTOR inhibitor 
      (Rapamycin) with different concentrations. After 24 h, the mRNA levels of Cbfalpha1 
      and OPN were determined and after 48 h, the protein expressions of p-Akt, p-mTOR, 
      Cbfalpha1 and OPN were quantified. Also, at day 7, calcium deposition was also 
      visualized by Alizarin stain. Results: After 7 days, compared with normal 
      phosphorus group, calcium deposition was more obvious in high phosphorus group. 
      The mRNA expressions of Cbfalpha1 and OPN increased significantly and the protein 
      expressions of p-Akt, p-mTOR, Cbfalpha1 and OPN up-regulated significantly in high 
      phosphorus group (all P<0.05). After treated with Wortmannin or Rapamycin for 24 
      h, compared with high phosphorus group, the mRNA expressions of Cbfalpha1 and OPN 
      decreased significantly in high phosphorus + Wortmannin (30, 50 and 100 nmol/L) 
      groups (all P<0.05) and high phosphorus + Rapamycin (1, 10 and 100 ng/ml) groups 
      (all P<0.05). After treated with Wortmannin or Rapamycin for 48 h, compared with 
      high phosphorus group, the protein expressions of p-Akt, Cbfalpha1 and OPN 
      down-regulated significantly in high phosphorus + Wortmannin (30, 50 and 100 
      nmol/L) groups (all P<0.05). It showed a dose-dependent down-regulation of 
      p-mTOR, Cbfalpha1 and OPN in high phosphorus + Rapamycin (1, 10, 100 ng/ml) groups 
      (all P<0.05). After 7 days, compared with high phosphorus group, calcium 
      deposition decresased significantly in high phosphorus + Wortmannin and high 
      phosphorus + Rapamycin groups. Conclusions: High phosphorus can induce VSMC 
      calcification. Akt and mTOR are involved in VSMC calcification induced by high 
      phosphorus through the activation of Cbfalpha1.
FAU - Wang, Y
AU  - Wang Y
AD  - Department of Blood Purification, Fuzhou General Hospital of PLA, Fuzhou 350025, 
      China.
FAU - Yu, Y
AU  - Yu Y
FAU - Zhang, H X
AU  - Zhang HX
FAU - Wu, X L
AU  - Wu XL
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Phosphates)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Muscle, Smooth, Vascular
MH  - Phosphates
MH  - Proto-Oncogene Proteins c-akt
MH  - Rats
MH  - Sirolimus
MH  - TOR Serine-Threonine Kinases
OTO - NOTNLM
OT  - Core binding factor alpha 1 subunit
OT  - Muscle, smooth, vascular
OT  - Proto-oncogene proteins c-akt
OT  - TOR serine-threonine kinases
EDAT- 2018/05/29 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/05/28 06:00
PHST- 2018/05/28 06:00 [entrez]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
AID - 10.3760/cma.j.issn.0376-2491.2018.18.016 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2018 May 15;98(18):1446-1451. doi: 
      10.3760/cma.j.issn.0376-2491.2018.18.016.