PMID- 29805692 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 1792-1074 (Print) IS - 1792-1082 (Electronic) IS - 1792-1074 (Linking) VI - 15 IP - 6 DP - 2018 Jun TI - Mps1 is associated with the BRAF(V600E) mutation but does not rely on the classic RAS/RAF/MEK/ERK signaling pathway in thyroid carcinoma. PG - 9978-9986 LID - 10.3892/ol.2018.8561 [doi] AB - In previous studies, the B-Raf proto-oncogene, serine/threonine kinase (BRAF)(V600E) mutation has been identified in multiple malignant tumors. BRAF(V600E) has been revealed to contribute to tumorigenesis by the activation of phospho-mitogen-activated protein kinases (MAPKs) and their downstream Monopolar spindle 1 (Mps1), leading to chromosome euploidy and tumor development. In the present study, the presence of phospho-MAPK and Mps1 in 161 thyroid carcinoma cases with complete clinical parameters was analyzed by immunohistochemistry, and the BRAF mutation was detected by polymerase chain reaction-direct sequencing. It was revealed that BRAF(V600E) was present in ~34% of thyroid cancer cases and was associated with age, clinical tumor stage and lymph node stage. However, the association of BRAF(V600E) with overall survival was not statistically significant. The expression of Mps1 was significantly increased in tumor tissues with BRAF(V600E), however, this did not affect the expression of phospho-MAPK in thyroid carcinomas. Collectively, the results of the present study suggested that BRAF(V600E) may regulate the expression of Mps1 in MAP kinase independent ways in thyroid carcinoma. Therefore, Mps1 expression is associated with BRAF(V600E) while the upstream signaling of phospho-MAPK has no relevance. The specific mechanisms of BRAF(V600E) and the unknown pathway associated with Mps1 exhibit potential for further study, and provide a theoretical basis for the molecular treatment of thyroid carcinoma. FAU - Li, Yike AU - Li Y AD - Department of General Surgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Zhang, Yanyan AU - Zhang Y AD - Department of General Surgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Xiao, Shuaishuai AU - Xiao S AD - Department of General Surgery, Affiliated Tumor Hospital of Shanxi Medical University, Taiyuan, Shanxi 030013, P.R. China. FAU - Kong, Pengzhou AU - Kong P AD - Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. AD - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Cheng, Caixia AU - Cheng C AD - Department of Pathology, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Shi, Ruyi AU - Shi R AD - Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. AD - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Wang, Fang AU - Wang F AD - Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. AD - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Zhang, Ling AU - Zhang L AD - Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. AD - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Wang, Juan AU - Wang J AD - Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. AD - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Jia, Zhiwu AU - Jia Z AD - Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. AD - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Wu, Shuai AU - Wu S AD - Department of General Surgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Liu, Yun AU - Liu Y AD - Department of General Surgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Guo, Jiansheng AU - Guo J AD - Department of General Surgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Cheng, Xiaolong AU - Cheng X AD - Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. AD - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Cui, Yongping AU - Cui Y AD - Translational Medicine Research Center, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. AD - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. FAU - Liu, Jing AU - Liu J AD - Department of General Surgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China. LA - eng PT - Journal Article DEP - 20180424 PL - Greece TA - Oncol Lett JT - Oncology letters JID - 101531236 PMC - PMC5958632 OTO - NOTNLM OT - B-Raf proto-oncogene serine/threonine kinaseV600E OT - MpS1 OT - immunohistochemistry OT - phospho-mitogen-activated protein kinases OT - thyroid carcinoma EDAT- 2018/05/29 06:00 MHDA- 2018/05/29 06:01 PMCR- 2018/04/24 CRDT- 2018/05/29 06:00 PHST- 2016/09/02 00:00 [received] PHST- 2017/07/20 00:00 [accepted] PHST- 2018/05/29 06:00 [entrez] PHST- 2018/05/29 06:00 [pubmed] PHST- 2018/05/29 06:01 [medline] PHST- 2018/04/24 00:00 [pmc-release] AID - OL-0-0-8561 [pii] AID - 10.3892/ol.2018.8561 [doi] PST - ppublish SO - Oncol Lett. 2018 Jun;15(6):9978-9986. doi: 10.3892/ol.2018.8561. Epub 2018 Apr 24.