PMID- 29806036
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2380-2367 (Print)
IS  - 2380-2367 (Electronic)
VI  - 4
IP  - 10
DP  - 2017 Jul
TI  - Measurement of Mitochondrial Mass by Flow Cytometry during Oxidative Stress.
PG  - 275-283
LID - 10.20455/ros.2017.839 [doi]
AB  - Properly assessing mitochondrial health is crucial to understand their role in 
      disease. MitoTracker green (MTG) and nonylacridine orange (NAO) are fluorescent 
      probes which have been commonly used to assess mitochondrial mass. This is based 
      on the assumption that both MTG and NAO accumulate in mitochondria regardless of 
      the mitochondrial transmembrane potential (DeltaPsi(m)). Here, we utilized flow 
      cytometry to evaluate the performance of these probes for assessment of 
      mitochondrial mass relative to forward (FSC) and side scatter (SSC) in human 
      peripheral blood lymphocytes (PBL). In isolated mitochondria, two subpopulations 
      were identified by FSC and SSC measurements which were matched to subpopulations 
      stained by MTG and NAO. The performance of these dyes was examined under 
      oxidative and nitrosative stress induced by rotenone and NOC-18 while 
      N-acetylcysteine (NAC) was employed as an antioxidant. Production of reactive 
      oxygen species (ROS) and DeltaPsi(m) were monitored in parallel. With respect to 
      representation of mitochondrial mass, neither MTG nor NAO was affected by DeltaPsi(m). 
      However, MTG showed significant correlation with cytosolic and mitochondrial ROS 
      production and nitrosative stress. Our data suggest that NAO may be more suitable 
      than MTG for assessment of mitochondrial mass by flow cytometry during oxidative 
      stress.
FAU - Doherty, Edward
AU  - Doherty E
AD  - Division of Rheumatology, Departments of Medicine, Microbiology and Immunology, 
      and Biochemistry and Molecular Biology, State University of New York, Upstate 
      Medical University, College of Medicine, Syracuse, NY 13210, USA.
FAU - Perl, Andras
AU  - Perl A
AD  - Division of Rheumatology, Departments of Medicine, Microbiology and Immunology, 
      and Biochemistry and Molecular Biology, State University of New York, Upstate 
      Medical University, College of Medicine, Syracuse, NY 13210, USA.
LA  - eng
GR  - R01 DK078922/DK/NIDDK NIH HHS/United States
GR  - R01 DK049221/DK/NIDDK NIH HHS/United States
GR  - R01 AI122176/AI/NIAID NIH HHS/United States
GR  - R01 AI048079/AI/NIAID NIH HHS/United States
GR  - R21 AI061066/AI/NIAID NIH HHS/United States
GR  - R01 AI072648/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20170701
PL  - United States
TA  - React Oxyg Species (Apex)
JT  - Reactive oxygen species (Apex, N.C.)
JID - 101688617
PMC - PMC5964986
MID - NIHMS967385
OTO - NOTNLM
OT  - Flow cytometry
OT  - MitoTracker green
OT  - Mitochondrial mass
OT  - Nonylacridine orange
OT  - Oxidative stress
EDAT- 2018/05/29 06:00
MHDA- 2018/05/29 06:01
PMCR- 2018/05/23
CRDT- 2018/05/29 06:00
PHST- 2018/05/29 06:00 [entrez]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/05/29 06:01 [medline]
PHST- 2018/05/23 00:00 [pmc-release]
AID - 10.20455/ros.2017.839 [doi]
PST - ppublish
SO  - React Oxyg Species (Apex). 2017 Jul;4(10):275-283. doi: 10.20455/ros.2017.839. 
      Epub 2017 Jul 1.