PMID- 29806089
OWN - NLM
STAT- MEDLINE
DCOM- 20190306
LR  - 20190306
IS  - 1743-7563 (Electronic)
IS  - 1743-7555 (Linking)
VI  - 15
IP  - 1
DP  - 2019 Feb
TI  - FISH analysis of selected soft tissue tumors: Diagnostic experience in a tertiary 
      center.
PG  - 38-47
LID - 10.1111/ajco.12980 [doi]
AB  - AIM: Fluorescence in situ hybridization (FISH) is an important ancillary tool for 
      the classification of bone/soft tissue (BST) tumors. The aim of this study was to 
      evaluate the contribution of FISH to the final classification of common BST 
      entities in the molecular pathology department of the Royal Prince Alfred 
      Hospital (RPAH), which is one of the most important referral centers for the 
      management of sarcomas in Australia. METHODS: All routine diagnostic FISH tests 
      performed on BST formalin-fixed paraffin embedded (FFPE) tissue specimens at the 
      RPAH in a 5-year period (February, 2010-November, 2015) were reviewed. FISH 
      analyses presented in this study include commercial break-apart probes (SS18, 
      FUS, DDIT3, FUS, USP6, PDGFB, TFE3 and ALK) and a single enumeration (MDM2) 
      probe. RESULTS: There were 434 interpretable FISH assays on BST samples including 
      MDM2 (n=180), SS18 (n=97), FUS (n=64), DDIT3 (n=37), USP6 (n=30), PDGFB (n=13), 
      TFE3 (n=8) and ALK (n=5). Discrepancies between the histopathological diagnosis 
      and the FISH results were seen in 12% of the cases. In this subset of discordant 
      cases, FISH contributed to the re-classification of 7% of cases originally 
      diagnosed as synovial sarcoma (SS18) and 6% of adipocytic neoplasms (MDM2) based 
      on the presence or absence of the expected gene alteration. CONCLUSION: Our study 
      confirms that paraffin FISH is a sensitive and specific ancillary tool in the 
      diagnosis of BST neoplasms when used in the appropriate clinicopathological 
      context. These findings highlight the need for further ancillary molecular tools 
      in the diagnosis and characterization of challenging cases.
CI  - (c) 2018 John Wiley & Sons Australia, Ltd.
FAU - Vargas, Ana Cristina
AU  - Vargas AC
AUID- ORCID: 0000-0002-9424-2080
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
FAU - Selinger, Christina
AU  - Selinger C
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
FAU - Satgunaseelan, Laveniya
AU  - Satgunaseelan L
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
AD  - Douglass Hanly Moir Pathology, Macquarie Park, New South Wales, Australia.
FAU - Cooper, Wendy A
AU  - Cooper WA
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
AD  - Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
AD  - School of Medicine, University of Western Sydney, Campbelltown, New South Wales, 
      Australia.
FAU - Gupta, Ruta
AU  - Gupta R
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
AD  - Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
FAU - Stalley, Paul
AU  - Stalley P
AD  - Chris O'Brien Lifehouse, Camperdown, New South Wales, Australia.
AD  - Orthopaedic Surgery, Royal Prince Alfred Hospital, Camperdown, New South Wales, 
      Australia.
AD  - Macquarie University, New South Wales, Australia.
AD  - Westmead Kids, Sydney, New South Wales, Australia.
AD  - North Shore Private Hospital, St Leonards, New South Wales, Australia.
FAU - Brown, Wendy
AU  - Brown W
AD  - Radiology/Medical Imaging, Royal Prince Alfred Hospital, Camperdown, New South 
      Wales, Australia.
FAU - Soper, Judy
AU  - Soper J
AD  - Radiology/Medical Imaging, Royal Prince Alfred Hospital, Camperdown, New South 
      Wales, Australia.
FAU - Schatz, Julie
AU  - Schatz J
AD  - Radiology/Medical Imaging, Royal Prince Alfred Hospital, Camperdown, New South 
      Wales, Australia.
FAU - Boyle, Richard
AU  - Boyle R
AD  - Chris O'Brien Lifehouse, Camperdown, New South Wales, Australia.
AD  - Orthopaedic Surgery, Royal Prince Alfred Hospital, Camperdown, New South Wales, 
      Australia.
AD  - Macquarie University, New South Wales, Australia.
AD  - Westmead Kids, Sydney, New South Wales, Australia.
AD  - North Shore Private Hospital, St Leonards, New South Wales, Australia.
FAU - Thomas, David M
AU  - Thomas DM
AD  - The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Darlinghurst, 
      New South Wales, Australia.
FAU - Tattersall, Martin H N
AU  - Tattersall MHN
AD  - Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
AD  - Chris O'Brien Lifehouse, Camperdown, New South Wales, Australia.
FAU - Bhadri, Vivek
AU  - Bhadri V
AD  - Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
AD  - Chris O'Brien Lifehouse, Camperdown, New South Wales, Australia.
FAU - Maclean, Fiona
AU  - Maclean F
AD  - Douglass Hanly Moir Pathology, Macquarie Park, New South Wales, Australia.
FAU - Bonar, Sally Fiona
AU  - Bonar SF
AD  - Douglass Hanly Moir Pathology, Macquarie Park, New South Wales, Australia.
AD  - Westmead Kids, Sydney, New South Wales, Australia.
AD  - School of Medicine, Notre Dame University, Sydney, New South Wales, Australia.
FAU - Scolyer, Richard A
AU  - Scolyer RA
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
AD  - Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
FAU - Karim, Rooshdiya Z
AU  - Karim RZ
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
AD  - Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
FAU - McCarthy, Stanley W
AU  - McCarthy SW
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
AD  - Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
FAU - Mahar, Annabelle
AU  - Mahar A
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
FAU - O'Toole, Sandra A
AU  - O'Toole SA
AD  - Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred 
      Hospital, Camperdown, New South Wales, Australia.
AD  - Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
AD  - The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Darlinghurst, 
      New South Wales, Australia.
LA  - eng
GR  - Cancer Institute NSW/
GR  - Breast Cancer Foundation/
GR  - APP 1088778/Cancer Council NSW/
PT  - Journal Article
DEP - 20180528
PL  - Australia
TA  - Asia Pac J Clin Oncol
JT  - Asia-Pacific journal of clinical oncology
JID - 101241430
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Australia/epidemiology
MH  - *DNA Copy Number Variations
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Proteins/*genetics
MH  - Soft Tissue Neoplasms/*diagnosis/epidemiology/genetics
MH  - Tertiary Care Centers
MH  - Young Adult
OTO - NOTNLM
OT  - amplification
OT  - fluorescence in-situ hybridization
OT  - rearrangement
EDAT- 2018/05/29 06:00
MHDA- 2019/03/07 06:00
CRDT- 2018/05/29 06:00
PHST- 2017/07/29 00:00 [received]
PHST- 2018/03/29 00:00 [accepted]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2019/03/07 06:00 [medline]
PHST- 2018/05/29 06:00 [entrez]
AID - 10.1111/ajco.12980 [doi]
PST - ppublish
SO  - Asia Pac J Clin Oncol. 2019 Feb;15(1):38-47. doi: 10.1111/ajco.12980. Epub 2018 
      May 28.