PMID- 29806265
OWN - NLM
STAT- MEDLINE
DCOM- 20180629
LR  - 20210930
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 31
IP  - 3
DP  - 2017 Mar 15
TI  - [Effect of short-term low-frequency electrical stimulation on nerve regeneration 
      of delayed nerve defect during operation].
PG  - 335-344
LID - 10.7507/1002-1892.201609024 [doi]
AB  - OBJECTIVE: To explore the effect of short-term low-frequency electrical 
      stimulation (SLES) during operation on nerve regeneration in delayed peripheral 
      nerve injury with long gap. METHODS: Thirty female adult Sprague Dawley rats, 
      weighing 160-180 g, were used to prepare 13-mm defect model by trimming the nerve 
      stumps. Then all rats were randomly divided into 2 groups, 15 rats in each group. 
      After nerve defect was bridged by the contralateral normal sciatic nerve, SLES 
      was applied in the experimental group, but was not in the control group. The 
      spinal cords and dorsal root ganglions (DRGs) were harvested to carry out 
      immunofluorescence histochemistry double staining for growth-associated proteins 
      43 (GAP-43) and brain-derived neurotrophic factor (BDNF) at 1, 2, and 7 days 
      after repair. Fluorogold (FG) retrograde tracing was performed at 3 months after 
      repair. The mid-portion regenerated segments were harvested to perform Meyer's 
      trichrome staining, immunofluorescence double staining for neurofilament (NF) and 
      soluble protein 100 (S-100) on the transversely or longitudinal sections at 3 
      months after repair. The segment of the distal sciatic nerve trunk was harvested 
      for electron microscopy and morphometric analyses to measure the diameter of the 
      myelinated axons, thickness of myelin sheaths, the G ratio, and the density of 
      the myelinated nerve fibers. The gastrocnemius muscles of the operated sides were 
      harvested to measure the relative wet weight ratios. Karnovsky-Root 
      cholinesterase staining of the motor endplate was carried out. RESULTS: In the 
      experimental group, the expressions of GAP-43 and BDNF were higher than those in 
      the control group at 1 and 2 days after repair. The number of labeled neurons in 
      the anterior horn of gray matter in the spinal cord and DRGs at the operated side 
      from the experimental group was more than that from the control group. Meyer's 
      trichrome staining, immunofluorescence double staining, and the electron 
      microscopy observation showed that the regenerated nerves were observed to 
      develop better in the experimental group than the control group. The relative wet 
      weight ratio of experimental group was significantly higher than that of the 
      control group ( t=4.633, P=0.000). The size and the shape of the motor endplates 
      in the experimental group were better than those in the control group. 
      CONCLUSION: SLES can promote the regeneration ability of the short-term (1 month) 
      delayed nerve injury with long gap to a certain extent.
FAU - Jiao, Haishan
AU  - Jiao H
AD  - Department of Basic Medicine, Suzhou Vocational Health College, Suzhou Jiangsu, 
      215009, P.R.China.jiaohaishan@szhct.edu.cn.
FAU - Xiao, Bo
AU  - Xiao B
AD  - Department of Basic Medicine, Suzhou Vocational Health College, Suzhou Jiangsu, 
      215009, P.R.China.
FAU - Wang, Xiaodong
AU  - Wang X
AD  - Department of Histology and Embryology, Medical College, Nantong University, 
      Nantong Jiangsu, 226001, P.R.China.
FAU - Li, Dongyin
AU  - Li D
AD  - Department of Basic Medicine, Suzhou Vocational Health College, Suzhou Jiangsu, 
      215009, P.R.China.
FAU - Song, Yuening
AU  - Song Y
AD  - Department of Basic Medicine, Suzhou Vocational Health College, Suzhou Jiangsu, 
      215009, P.R.China.
FAU - Zheng, Hui
AU  - Zheng H
AD  - Department of Basic Medicine, Suzhou Vocational Health College, Suzhou Jiangsu, 
      215009, P.R.China.
FAU - Liu, Xiaomei
AU  - Liu X
AD  - Department of Basic Medicine, Suzhou Vocational Health College, Suzhou Jiangsu, 
      215009, P.R.China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
SB  - IM
MH  - Animals
MH  - Axons
MH  - *Electric Stimulation
MH  - Female
MH  - *Nerve Regeneration
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/*growth & development
PMC - PMC8458119
OTO - NOTNLM
OT  - Short-time low-frequency electrical stimulation
OT  - chronic injury
OT  - nerve defect repair
OT  - peripheral nerve
OT  - rat
EDAT- 2017/03/01 00:00
MHDA- 2018/06/30 06:00
PMCR- 2017/03/01
CRDT- 2018/05/29 06:00
PHST- 2018/05/29 06:00 [entrez]
PHST- 2017/03/01 00:00 [pubmed]
PHST- 2018/06/30 06:00 [medline]
PHST- 2017/03/01 00:00 [pmc-release]
AID - zgxfcjwkzz-31-3-335 [pii]
AID - 10.7507/1002-1892.201609024 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2017 Mar 15;31(3):335-344. doi: 
      10.7507/1002-1892.201609024.