PMID- 29806300
OWN - NLM
STAT- MEDLINE
DCOM- 20180831
LR  - 20210910
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 32
IP  - 4
DP  - 2018 Apr 15
TI  - [Effect of bone morphogenetic protein 7/poly (lactide-co-glycolide) microspheres 
      on the in vitro proliferation and chondrogenic differentiation of rabbit bone 
      marrow mesenchymal stem cells].
PG  - 428-433
LID - 10.7507/1002-1892.201711093 [doi]
AB  - OBJECTIVE: To evaluate the effect of bone morphogenetic protein 7 (BMP-7)/poly 
      (lactide-co-glycolide) (PLGA) microspheres on in vitro proliferation and 
      chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells 
      (BMSCs). METHODS: BMP-7/PLGA microspheres were fabricated by double 
      emulsion-drying in liquid method. After mixing BMP-7/PLGA microspheres with the 
      chondrogenic differentiation medium, the supernatant was collected on the 1st, 
      3rd, 7th, 14th, and 21st day as the releasing solution. The BMSCs were isolated 
      from the bilateral femurs and tibias of 3-5 days old New Zealand rabbits, and the 
      3rd generation BMSCs were divided into 2 groups: microspheres group and control 
      group. The BMSCs in microspheres group were cultured by 200 muL BMP-7/PLGA 
      microspheres releasing solution in the process of changing liquid every 2-3 days, 
      while in control group were cultured by chondrogenic medium. The cell 
      proliferation (by MTT assay) and the glycosaminoglycan (GAG) contents (by Alician 
      blue staining) were detected after chondrogenic cultured for 1, 3, 7, 14, and 21 
      days. The chondrogenic differentiation of BMSCs was observed by safranine O 
      staining, toluidine blue staining, and collagen type Ⅱ immunohistochemistry 
      staining at 21 days. RESULTS: MTT test showed that BMSCs proliferated rapidly in 
      2 groups at 1, 3, and 7 days; after 7 days, the proliferation of BMSCs in the 
      control group was slow and the BMSCs in microspheres group continued to 
      proliferate rapidly. There was no significant difference of the absorbance ( A) 
      value at 1, 3, and 7 days between 2 groups ( P>0.05), but the A value at 14 and 
      21 days in microspheres group was significantly higher than that in control group 
      ( P<0.05). Compared with control group at 21 days, in microsphere group, almost 
      all nuclei were dyed bright red by safranine O staining, almost all the nuclei 
      appeared metachromatic purple red by toluidine blue staining, and the most nuclei 
      were yellow or brown by immunohistochemical staining of collagen type Ⅱ. Alcian 
      blue staining showed that the content of GAG in 2 groups increased continuously 
      at different time points; after 7 days, the increasing trend of the control group 
      was slow and the microspheres group continued hypersecretion. There was no 
      significant difference of the GAG content at 1, 3, and 7 days between 2 groups ( 
      P>0.05), but the GAG content at 14 and 21 days in microspheres group was 
      significantly higher than that in control group ( P<0.05). CONCLUSION: BMP-7/PLGA 
      microspheres prepared by double emulsion-drying in liquid method in vitro can 
      promote proliferation and chondrogenic differentiation of rabbit BMSCs.
FAU - Chen, Jialei
AU  - Chen J
AD  - Department of Orthopeadics, West China Hospital, Sichuan University, Chengdu 
      Sichuan, 610041, P.R.China.
FAU - Liu, Ming
AU  - Liu M
AD  - Department of Orthopeadics, West China Hospital, Sichuan University, Chengdu 
      Sichuan, 610041, P.R.China.
FAU - Duan, Xin
AU  - Duan X
AD  - Department of Orthopeadics, West China Hospital, Sichuan University, Chengdu 
      Sichuan, 610041, P.R.China.
FAU - Huang, Fuguo
AU  - Huang F
AD  - Department of Orthopeadics, West China Hospital, Sichuan University, Chengdu 
      Sichuan, 610041, P.R.China.
FAU - Xiang, Zhou
AU  - Xiang Z
AD  - Department of Orthopeadics, West China Hospital, Sichuan University, Chengdu 
      Sichuan, 610041, P.R.China.xiangzhou15@hotmail.com.
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (BMP7 protein, human)
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Collagen Type II)
RN  - 0 (Dioxanes)
RN  - 0 (Polyesters)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 95-96-5 (dilactide)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - *Bone Morphogenetic Protein 7
MH  - Cell Differentiation
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Chondrogenesis
MH  - Collagen Type II
MH  - Dioxanes
MH  - *Mesenchymal Stem Cells
MH  - *Microspheres
MH  - Polyesters
MH  - Rabbits
MH  - *Tissue Engineering
PMC - PMC8414336
OTO - NOTNLM
OT  - Bone morphogenetic protein 7
OT  - bone marrow mesenchymal stem cells
OT  - microspheres
OT  - poly (lactide-co-glycolide)
OT  - rabbit
EDAT- 2018/05/29 06:00
MHDA- 2018/09/01 06:00
PMCR- 2018/04/01
CRDT- 2018/05/29 06:00
PHST- 2018/05/29 06:00 [entrez]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/09/01 06:00 [medline]
PHST- 2018/04/01 00:00 [pmc-release]
AID - zgxfcjwkzz-32-4-428 [pii]
AID - 10.7507/1002-1892.201711093 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Apr 15;32(4):428-433. doi: 
      10.7507/1002-1892.201711093.