PMID- 29806345
OWN - NLM
STAT- MEDLINE
DCOM- 20181126
LR  - 20210915
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 32
IP  - 5
DP  - 2018 May 15
TI  - [Effects of FTY720-P on EphA2-EphrinA2 bidirectional signaling in osteoclasts].
PG  - 575-580
LID - 10.7507/1002-1892.201710109 [doi]
AB  - OBJECTIVE: To investigate the effects of FTY720-P on EphA2-EphrinA2 bidirectional 
      signaling in osteoclasts. METHODS: Murine RAW264.7 macrophages were induced into 
      osteoclasts by dexamethasone and 1alpha, 25-dihydroxyvitamin D (3), and identified by 
      tartrate resistant acid phosphatase (TRAP) staining. Then, the osteoclasts were 
      divided into 2 groups. The osteoclasts were treated with 400 ng/mL FTY720-P in 
      experimental group and without FTY720-P in control group, respectively. After 48 
      hours of culture, the cells in 2 groups were detected by real-time fluorescent 
      quantitative PCR, Western blot, and immunofluorescence staining. The expressions 
      of EphA2, EphrinA2, RhoA, and the bone reconstruction associated proteins［bone 
      morphogenetic protein 2 (BMP-2) and transform growth factor beta (1) (TGF-beta 
      (1))］were analyzed and compared. RESULTS: RAW264.7 cells were successfully 
      induced into osteoclasts identified by TRAP staining. Compared with control 
      group, the relative expressions of EphA2 and EphrinA2 mRNAs and proteins in 
      experimental group significantly decreased after 48 hours ( P<0.05), and the 
      relative expression of RhoA protein also significantly decreased ( P<0.05). The 
      relative expressions of BMP-2 and TGF-beta (1) mRNAs were significantly increased ( 
      P<0.05), and those protein expressions were enhanced. CONCLUSION: FTY720-P can 
      down-regulate the expression of RhoA and promote the expressions of TGF- beta (1) 
      and BMP-2 by affecting the transduction of EphA2-EphrinA2 bidirectional signaling 
      in osteoclasts.
FAU - Zheng, Libin
AU  - Zheng L
AD  - Guangdong Medical University, Zhanjiang Guangdong, 524023, P.R.China.
FAU - Huang, Dong
AU  - Huang D
AD  - Guangdong Medical University, Zhanjiang Guangdong, 524023, P.R.China;Department 
      of Trauma Orthopedics, Guangdong Second Provincial Central Hospital, Guangzhou 
      Guangdong, 510000, P.R.China.dong_177@126.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Ephrin-A2)
RN  - 0 (FTY 720P)
RN  - 0 (Organophosphates)
RN  - EC 2.7.10.1 (Receptor, EphA2)
RN  - NGZ37HRE42 (Sphingosine)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 2
MH  - *Cell Differentiation
MH  - Ephrin-A2/*metabolism
MH  - Macrophages
MH  - Mice
MH  - Organophosphates/*metabolism
MH  - Osteoclasts/*metabolism
MH  - Receptor, EphA2/*metabolism
MH  - *Signal Transduction
MH  - Sphingosine/*analogs & derivatives/metabolism
PMC - PMC8430003
OTO - NOTNLM
OT  - EphA2
OT  - EphrinA2
OT  - FTY720-P
OT  - RhoA
OT  - osteoclasts
EDAT- 2018/05/29 06:00
MHDA- 2018/11/27 06:00
PMCR- 2018/05/01
CRDT- 2018/05/29 06:00
PHST- 2018/05/29 06:00 [entrez]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/11/27 06:00 [medline]
PHST- 2018/05/01 00:00 [pmc-release]
AID - zgxfcjwkzz-32-5-575 [pii]
AID - 10.7507/1002-1892.201710109 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 May 15;32(5):575-580. doi: 
      10.7507/1002-1892.201710109.