PMID- 29806395
OWN - NLM
STAT- MEDLINE
DCOM- 20180604
LR  - 20211015
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 31
IP  - 12
DP  - 2017 Dec 15
TI  - [Effect of bone marrow mesenchymal stem cells conditioned medium on microglia and 
      its secretion of arginase 1 in rats].
PG  - 1500-1505
LID - 10.7507/1002-1892.201710038 [doi]
AB  - OBJECTIVE: To observe the effect of bone marrow mesenchymal stem cells (BMSCs) 
      conditioned medium on microglia (MGs) and its secretion of arginase 1 (Arg1). 
      METHODS: The BMSCs separated through differential adhesion method from the femur 
      and tibia marrow of 4-week-old Sprague Dawley (SD) rats were cultured and 
      identified by Vimentin immunofluorescence staining; whereas MGs separated through 
      trypsin digestion method from the brain of 3-day-old SD rats were cultured and 
      identified by Iba1 immunofluorescence staining. The primary MGs were cultured 
      with DMEM/F12 medium containing BMSCs conditioned medium (experimental group) and 
      with single DMEM/F12 medium (control group), respectively. After 48 hours of 
      culture, the morphology of MGs was observed by inverted phase contrast 
      microscope, the activated state of MGs was detected by using Iba1 
      immunofluorescence staining, and Arg1 expression of MGs was assessed by Iba1-Arg1 
      double-labelling immunofluorescence staining and Western blot method. RESULTS: 
      Inverted phase contrast microscope observation showed that BMSCs entered 
      logarithmic growth phase at 14 days after culture, and more than 98% cells were 
      positive to Vimentin immunofluorescence staining; whereas MGs entered logarithmic 
      growth phase at 21 days after culture, and around 80% cells were positive to Iba1 
      immunofluorescence staining. Inverted phase contrast microscope observation 
      displayed that in the experimental group, MGs were activated with increased size 
      of soma, shortened process, and amoeba change. Immunofluorescence staining 
      displayed that the Iba1 positive cells number in the experimental group was 
      significantly higher than that in the control group ( t=0.007, P=0.000); 
      double-labelling immunofluorescence staining revealed that the Iba1-Arg1 positive 
      cells number in the experimental group was significantly higher than that in the 
      control group ( t=0.007, P=0.000); and Western blot results elucidated that the 
      relative expression of Arg1 protein in the experimental group was significantly 
      higher than that in the control group ( t=0.001, P=0.000). CONCLUSION: BMSCs 
      conditioned medium can activate MGs and induce MGs to express Arg1.
FAU - Chen, Yunhui
AU  - Chen Y
AD  - College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, 
      Chengdu Sichuan, 610075, P.R.China.
FAU - Yu, Shuguang
AU  - Yu S
AD  - College of Acupuncture and Moxibustion of Chengdu University of Traditional 
      Chinese Medicine, Chengdu Sichuan, 610075, P.R.China.
FAU - Wu, Qiaofeng
AU  - Wu Q
AD  - College of Acupuncture and Moxibustion of Chengdu University of Traditional 
      Chinese Medicine, Chengdu Sichuan, 610075, P.R.China.
FAU - Tang, Yong
AU  - Tang Y
AD  - College of Acupuncture and Moxibustion of Chengdu University of Traditional 
      Chinese Medicine, Chengdu Sichuan, 610075, P.R.China.
FAU - Jiang, Cen
AU  - Jiang C
AD  - College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, 
      Chengdu Sichuan, 610075, P.R.China.
FAU - Zhuang, Zhiqi
AU  - Zhuang Z
AD  - College of Acupuncture and Moxibustion of Chengdu University of Traditional 
      Chinese Medicine, Chengdu Sichuan, 610075, P.R.China.
FAU - Zhao, Na
AU  - Zhao N
AD  - College of Acupuncture and Moxibustion of Chengdu University of Traditional 
      Chinese Medicine, Chengdu Sichuan, 610075, P.R.China.
FAU - Huang, Biao
AU  - Huang B
AD  - College of Acupuncture and Moxibustion of Chengdu University of Traditional 
      Chinese Medicine, Chengdu Sichuan, 610075, P.R.China.
FAU - Xie, Lushuang
AU  - Xie L
AD  - College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, 
      Chengdu Sichuan, 610075, P.R.China;College of Acupuncture and Moxibustion of 
      Chengdu University of Traditional Chinese Medicine, Chengdu Sichuan, 610075, 
      P.R.China.xiescarlett418@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Culture Media, Conditioned)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Animals
MH  - Arginase/*metabolism
MH  - Bone Marrow Cells
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - *Mesenchymal Stem Cells
MH  - *Microglia
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC8498264
OTO - NOTNLM
OT  - Bone marrow mesenchymal stem cells
OT  - arginase 1
OT  - microglia
OT  - rat
EDAT- 2018/05/29 06:00
MHDA- 2018/06/05 06:00
PMCR- 2017/12/01
CRDT- 2018/05/29 06:00
PHST- 2018/05/29 06:00 [entrez]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/06/05 06:00 [medline]
PHST- 2017/12/01 00:00 [pmc-release]
AID - zgxfcjwkzz-31-12-1500 [pii]
AID - 10.7507/1002-1892.201710038 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2017 Dec 15;31(12):1500-1505. doi: 
      10.7507/1002-1892.201710038.