PMID- 29807336
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20211204
IS  - 1873-3557 (Electronic)
IS  - 1386-1425 (Linking)
VI  - 202
DP  - 2018 Sep 5
TI  - A label-free aptasensor for the detection of tetracycline based on the 
      luminescence of SYBR Green I.
PG  - 382-388
LID - S1386-1425(18)30471-2 [pii]
LID - 10.1016/j.saa.2018.05.075 [doi]
AB  - A novel fluorescent method based on tetracycline-binding aptamers and the 
      luminescence of SYBR Green I (SGI) was established for the sensitive and 
      selective detection of tetracycline. Under natural conditions, the aptamers of 
      tetracycline show the G-quadruplex spatial structures while SGI is nearly 
      nonfluorescent in aqueous solution. After mixture with the G-quadruplex 
      structured aptamers, SGI can recognize and intercalate into the aptamers, 
      resulting in a strong fluorescence emission. When the target tetracycline was 
      added into the solution, the specific recognition and high-affinity binding of 
      aptamers with tetracycline will induce the conformational changes of aptamers 
      from G-quadruplex structures to hairpin structures. Thereafter, SGI will be 
      released from the aptamer molecules, leading to the fluorescence decline. The 
      quantitative detection of tetracycline can be achieved by measuring the 
      fluorescence change of the system. Under the optimum conditions, the linear range 
      of tetracycline in the milk was from 5 to 25 mug/mL, and the detection limit was 
      as low as 0.10 mug/mL. The recoveries of the spiked milk samples were in the range 
      of 98.98%-104.67% with the relative standard deviations (RSDs) of 0.16%-0.67%, 
      and the results were in agreement with those from HPLC. Therefore, the biosensor 
      based on the specific recognition of aptamers and the fluorescence properties of 
      SGI can detect the tetracycline in milk accurately, rapidly and specifically.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Yang, Chuanyu
AU  - Yang C
AD  - Department of Food Quality and Safety, College of Food Science and Engineering, 
      Jilin University, Changchun 130062, China.
FAU - Bie, Jiaxin
AU  - Bie J
AD  - Department of Food Quality and Safety, College of Food Science and Engineering, 
      Jilin University, Changchun 130062, China.
FAU - Zhang, Xinmeng
AU  - Zhang X
AD  - Department of Food Quality and Safety, College of Food Science and Engineering, 
      Jilin University, Changchun 130062, China.
FAU - Yan, Caiyun
AU  - Yan C
AD  - Department of Food Quality and Safety, College of Food Science and Engineering, 
      Jilin University, Changchun 130062, China.
FAU - Li, Hanjie
AU  - Li H
AD  - Department of Food Quality and Safety, College of Food Science and Engineering, 
      Jilin University, Changchun 130062, China.
FAU - Zhang, Minghui
AU  - Zhang M
AD  - Department of Food Quality and Safety, College of Food Science and Engineering, 
      Jilin University, Changchun 130062, China.
FAU - Su, Ruifang
AU  - Su R
AD  - Department of Food Quality and Safety, College of Food Science and Engineering, 
      Jilin University, Changchun 130062, China.
FAU - Zhang, Xiaoguang
AU  - Zhang X
AD  - Department of Food Quality and Safety, College of Food Science and Engineering, 
      Jilin University, Changchun 130062, China. Electronic address: 
      xiaoguang61@jlu.edu.cn.
FAU - Sun, Chunyan
AU  - Sun C
AD  - Department of Food Quality and Safety, College of Food Science and Engineering, 
      Jilin University, Changchun 130062, China. Electronic address: 
      sunchuny@jlu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180521
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Benzothiazoles)
RN  - 0 (Diamines)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Organic Chemicals)
RN  - 0 (Pharmaceutical Preparations)
RN  - 0 (Quinolines)
RN  - 0 (Tetracyclines)
RN  - 163795-75-3 (SYBR Green I)
SB  - IM
MH  - Animals
MH  - Aptamers, Nucleotide/*chemistry
MH  - Benzothiazoles
MH  - *Biosensing Techniques
MH  - Circular Dichroism
MH  - Diamines
MH  - Fluorescence
MH  - Fluorescent Dyes/chemical synthesis/chemistry
MH  - *Luminescence
MH  - Milk/chemistry
MH  - Organic Chemicals/*chemistry
MH  - Pharmaceutical Preparations/analysis/chemistry
MH  - Quinolines
MH  - Spectrometry, Fluorescence
MH  - Tetracyclines/*analysis
OTO - NOTNLM
OT  - Aptamers
OT  - Fluorescence
OT  - SYBR Green I
OT  - Tetracycline
EDAT- 2018/05/29 06:00
MHDA- 2018/10/16 06:00
CRDT- 2018/05/29 06:00
PHST- 2018/04/08 00:00 [received]
PHST- 2018/05/17 00:00 [revised]
PHST- 2018/05/20 00:00 [accepted]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PHST- 2018/05/29 06:00 [entrez]
AID - S1386-1425(18)30471-2 [pii]
AID - 10.1016/j.saa.2018.05.075 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2018 Sep 5;202:382-388. doi: 
      10.1016/j.saa.2018.05.075. Epub 2018 May 21.