PMID- 29808298
OWN - NLM
STAT- MEDLINE
DCOM- 20180814
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 410
IP  - 20
DP  - 2018 Aug
TI  - Chiral capillary electrophoresis with UV-excited fluorescence detection for the 
      enantioselective analysis of 9-fluorenylmethoxycarbonyl-derivatized amino acids.
PG  - 4979-4990
LID - 10.1007/s00216-018-1148-x [doi]
AB  - The potential of capillary electrophoresis (CE) with ultraviolet (UV)-excited 
      fluorescence detection for sensitive chiral analysis of amino acids (AAs) was 
      investigated. DL-AAs were derivatized with 9-fluorenylmethoxycarbonyl chloride 
      (FMOC)-Cl to allow their fluorescence detection and enhance enantioseparation. 
      Fluorescence detection was achieved employing optical fibers, leading UV 
      excitation light (< 300 nm) from a Xe-Hg lamp to the capillary window, and 
      fluorescence emission to a spectrograph equipped with a charge-coupled device 
      (CCD). Signal averaging over time and emission wavelength intervals was carried 
      out to improve the signal-to-noise ratio of the FMOC-AAs. A background 
      electrolyte (BGE) of 40 mM sodium tetraborate (pH 9.5), containing 15% 
      isopropanol (v/v), 30 mM sodium dodecyl sulfate (SDS), and 30 mM beta-cyclodextrin 
      (beta-CD), was found optimal for AA chemo- and enantioseparation. Enantioresolutions 
      of 1.0 or higher were achieved for 16 proteinogenic DL-AAs. Limits of detection 
      (LODs) were in the 10-100-nM range (injected concentration) for the D-AA 
      enantiomers, except for FMOC-D-tryptophan (536 nM) which showed intramolecular 
      fluorescence quenching. Linearity (R(2) > 0.997) and repeatability for peak 
      height (relative standard deviations (RSDs) < 7.0%; n = 5) and electrophoretic 
      mobility (RSDs < 0.6%; n = 5) of individual AA enantiomers were established for 
      chiral analysis of DL-AA mixtures. The applicability of the method was 
      investigated by the analysis of cerebrospinal fluid (CSF). Next to L-AAs, 
      endogenous levels of D-glutamine and D-aspartic acid could be measured in CSF 
      revealing enantiomeric ratios of 0.35 and 19.6%, respectively. This indicates the 
      method's potential for the analysis of low concentrations of D-AAs in presence of 
      abundant L-AAs.
FAU - Prior, Amir
AU  - Prior A
AD  - Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules, Medicines 
      and Systems, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV, Amsterdam, 
      The Netherlands.
FAU - Coliva, Giulia
AU  - Coliva G
AD  - Biomolecular Analysis, Utrecht University, Universiteitsweg 99, 3584 CG, Utrecht, 
      The Netherlands.
FAU - de Jong, Gerhardus J
AU  - de Jong GJ
AD  - Biomolecular Analysis, Utrecht University, Universiteitsweg 99, 3584 CG, Utrecht, 
      The Netherlands.
FAU - Somsen, Govert W
AU  - Somsen GW
AD  - Division of BioAnalytical Chemistry, Amsterdam Institute for Molecules, Medicines 
      and Systems, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV, Amsterdam, 
      The Netherlands. g.w.somsen@vu.nl.
LA  - eng
GR  - ECHO project 711.011.003/Nederlandse Organisatie voor Wetenschappelijk Onderzoek/
PT  - Evaluation Study
PT  - Journal Article
DEP - 20180529
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (9-fluorenylmethoxycarbonyl)
RN  - 0 (Amino Acids)
RN  - 0 (Fluorenes)
RN  - 0 (Fluorescent Dyes)
RN  - 9PLB0BTT90 (1-(9-fluorenyl)methyl chloroformate)
SB  - IM
MH  - Amino Acids/analysis/*cerebrospinal fluid
MH  - Electrophoresis, Capillary/*methods
MH  - Fluorenes/*analysis
MH  - Fluorescence
MH  - Fluorescent Dyes/*analysis
MH  - Humans
MH  - Limit of Detection
MH  - Spectrometry, Fluorescence/methods
MH  - Stereoisomerism
PMC - PMC6061710
OTO - NOTNLM
OT  - Amino acids
OT  - Capillary electrophoresis
OT  - Cerebrospinal fluid
OT  - Chiral separation
OT  - FMOC derivatization
OT  - Fluorescence detection
COIS- The authors declare no conflict of interest.
EDAT- 2018/05/29 06:00
MHDA- 2018/08/15 06:00
PMCR- 2018/05/29
CRDT- 2018/05/30 06:00
PHST- 2018/02/21 00:00 [received]
PHST- 2018/05/16 00:00 [accepted]
PHST- 2018/05/14 00:00 [revised]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/08/15 06:00 [medline]
PHST- 2018/05/30 06:00 [entrez]
PHST- 2018/05/29 00:00 [pmc-release]
AID - 10.1007/s00216-018-1148-x [pii]
AID - 1148 [pii]
AID - 10.1007/s00216-018-1148-x [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2018 Aug;410(20):4979-4990. doi: 10.1007/s00216-018-1148-x. 
      Epub 2018 May 29.