PMID- 29808799
OWN - NLM
STAT- MEDLINE
DCOM- 20181107
LR  - 20191210
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 64
IP  - 6
DP  - 2018 May 15
TI  - miR-140-5p suppresses retinoblastoma cell proliferation, migration, and invasion 
      by targeting CEMIP and CADM3.
PG  - 42-47
AB  - Retinoblastoma (RB) is a childhood intraocular tumor, affecting millions of 
      patients worldwide. MicroRNA-140-5p (miR-140-5p) was demonstrated to be involved 
      in the tumorigenesis of various human cancers; however, its role in RB remains 
      undetermined. In this study, quantitative real-time PCR (qRT-PCR) and Western 
      blot assays were used to determine the expression levels of miR-140-5p, cell 
      migration-inducing protein (CEMIP), and cell adhesion molecule 3 (CADM3) in RB 
      tissues and cell-lines. The proliferation ability was detected by cell-counting 
      kit 8 (CCK-8), Edu staining, and colony formation assay. The cell cycle and 
      migration and invasion abilities were measured by flow cytometry, wound-healing 
      assay and Transwell assays, respectively. The correlation between miR-140-5p and 
      CEMIP/CADM3 were then confirmed by immunofluorescence (IF) and dual-luciferase 
      reporter assays. The results showed that miR-140-5p expression was significantly 
      decreased; however, CEMIP and CADM3 expression was increased in RB tissues and 
      cells. Overexpression of miR-140-5p inhibited proliferation, migration, and 
      invasion of RB cells. We also found that miR-140-5p inhibited CEMIP and CADM3 
      expressions in RB cells. In addition, we demonstrated that miR-140-5p might 
      negatively regulate the transcriptional activities of CEMIP and CADM3 by 
      targeting their 3'-UTR. Therefore, we suggested that miR-140-5p could be a 
      potential therapeutic target for the treatment of RB through CEMIP and CADM3.
FAU - Miao, Xiaolin
AU  - Miao X
AD  - Outpatient Department, Eye Hospital of Wenzhou Medical University, China.
FAU - Wang, Zhen
AU  - Wang Z
AD  - Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory 
      of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital 
      of Hangzhou medical College, China.
FAU - Chen, Bingyu
AU  - Chen B
AD  - Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory 
      of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital 
      of Hangzhou medical College, China.
FAU - Chen, Yiqi
AU  - Chen Y
AD  - Outpatient Department, Eye Hospital of Wenzhou Medical University, China.
FAU - Wang, Xi
AU  - Wang X
AD  - Key Laboratory of Digestive Pathophysiology of Zhejiang Province, Zhejiang 
      Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, 
      China.
FAU - Jiang, Luxi
AU  - Jiang L
AD  - Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory 
      of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital 
      of Hangzhou medical College, China.
FAU - Jiang, Shanshan
AU  - Jiang S
AD  - Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory 
      of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital 
      of Hangzhou medical College, China.
FAU - Hao, Ke
AU  - Hao K
AD  - Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory 
      of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital 
      of Hangzhou medical College, China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory 
      of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital 
      of Hangzhou medical College, China.
LA  - eng
PT  - Journal Article
DEP - 20180515
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (CADM3 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Immunoglobulins)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn140 microRNA, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.2.1.35 (CEMIP protein, human)
RN  - EC 3.2.1.35 (Hyaluronoglucosaminidase)
SB  - IM
MH  - Cell Adhesion Molecules/*antagonists & inhibitors/metabolism
MH  - Cell Division
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Eye Neoplasms/genetics/*pathology
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, Reporter
MH  - Humans
MH  - Hyaluronoglucosaminidase
MH  - Immunoglobulins/metabolism
MH  - MicroRNAs/genetics/*physiology
MH  - Molecular Targeted Therapy
MH  - Neoplasm Invasiveness
MH  - Neoplasm Proteins/antagonists & inhibitors/genetics/*physiology
MH  - Proteins/*antagonists & inhibitors/metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - RNA, Neoplasm/biosynthesis/genetics/*physiology
MH  - Recombinant Proteins/metabolism
MH  - Retinoblastoma/genetics/*pathology
MH  - Tumor Cells, Cultured
MH  - Tumor Stem Cell Assay
OTO - NOTNLM
OT  - Cell adhesion molecule 3
OT  - Cell migration inducing protein
OT  - MicroRNA-140-5p
OT  - Migration and invasion.
OT  - Proliferation
OT  - Retinoblastoma
EDAT- 2018/05/29 06:00
MHDA- 2018/11/08 06:00
CRDT- 2018/05/30 06:00
PHST- 2018/02/07 00:00 [received]
PHST- 2018/04/03 00:00 [accepted]
PHST- 2018/04/03 00:00 [revised]
PHST- 2018/05/30 06:00 [entrez]
PHST- 2018/05/29 06:00 [pubmed]
PHST- 2018/11/08 06:00 [medline]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2018 May 15;64(6):42-47.