PMID- 2981659
OWN - NLM
STAT- MEDLINE
DCOM- 19850319
LR  - 20131121
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 6
IP  - 1
DP  - 1985 Jan
TI  - Automatic cell identification and enrichment in lung cancer: V. Adenocarcinoma 
      and large cell undifferentiated carcinoma.
PG  - 37-46
AB  - The aims of this study were to develop a protocol for the identification and 
      enrichment of cancer cells from sputum obtained from patients with adenocarcinoma 
      of the lung (n = 6) and large-cell undifferentiated carcinoma of the lung (n = 
      2), and to compare these findings with the results from our previous studies on 
      other cell types from lung cancer. The hypotheses tested were: Cancer cells in 
      sputum can be preserved following flow sorting. Enrichment for cancer cells from 
      acridine orange (AO)-stained specimens can be achieved. Discrimination of cancer 
      cells from noncancer cells is by AO green fluorescence and discrimination of 
      lymphocytes from other cell types is by AO red fluorescence. Cancer cells are 
      consistently enriched in the AO high green and red fluorescence region, although, 
      for a given cell type, maximal enrichment is patient-dependent. Finally, cancer 
      cell enrichment and lymphocyte exclusion can be done simultaneously. Cells from 
      sputum were initially fixed, stained with AO, sorted on a dual parameter flow 
      sorter, and classified into six groups corresponding to two ranges of green and 
      three ranges of red fluorescence intensities. Cells of each region were stained 
      by the method of Papanicolaou and differential counts were performed to determine 
      the relative frequencies (i.e., purities) of leukocytes, macrophages, squamous 
      cells, and cancer cells, in sorted and unsorted (i.e., control) samples. The 
      average purity of leukocytes (81%), macrophages (6%), squamous cells (11%), and 
      cancer cells (2%) varied markedly from sample to sample. However, the largest 
      enrichment values (i.e., ratio of purity of a cell type in a sorted sample to its 
      purity in the unsorted control sample) achieved for cancer cells consistently 
      occurred for each patient sample in the region corresponding to high green and 
      high red fluorescence intensities. Experimentally, a cancer cell average 
      enrichment of sixteen-fold was obtained by this method. Additionally, 
      fluorescence intensity ranges which increased the enrichment for macrophages by 
      cell sorting typically excluded leukocytes and squamous cells, and vice versa. 
      Finally, red fluorescence intensity was the primary discriminatory parameter for 
      all cell types studied, although the additional use of green fluorescence 
      intensity significantly increased cancer cell enrichment rates.(ABSTRACT 
      TRUNCATED AT 400 WORDS)
FAU - Tyrer, H W
AU  - Tyrer HW
FAU - Pressman, N J
AU  - Pressman NJ
FAU - Albright, C D
AU  - Albright CD
FAU - Frost, J K
AU  - Frost JK
LA  - eng
GR  - CA-28706/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Adenocarcinoma/*pathology
MH  - Carcinoma, Small Cell/*pathology
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Leukocytes/pathology
MH  - Lung Neoplasms/*pathology
MH  - Macrophages/pathology
MH  - Sputum/cytology
EDAT- 1985/01/01 00:00
MHDA- 1985/01/01 00:01
CRDT- 1985/01/01 00:00
PHST- 1985/01/01 00:00 [pubmed]
PHST- 1985/01/01 00:01 [medline]
PHST- 1985/01/01 00:00 [entrez]
AID - 10.1002/cyto.990060108 [doi]
PST - ppublish
SO  - Cytometry. 1985 Jan;6(1):37-46. doi: 10.1002/cyto.990060108.