PMID- 2982628
OWN - NLM
STAT- MEDLINE
DCOM- 19850424
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 157
IP  - 1
DP  - 1985 Mar
TI  - Similar sensitivity of human lymphoblast cells proficient or deficient in 
      nucleotide biosynthesis to the cytotoxic and cell cycle effects of 
      3-aminobenzamide.
PG  - 271-5
AB  - 3-Aminobenzamide (3AB), a potent inhibitor of poly(ADP-ribose) polymerase, 
      induces cytotoxicity and cell cycle delays in exposed cells. 3AB has been 
      reported to inhibit de novo nucleotide synthesis in human lymphoblasts. To 
      determine if the 3AB-mediated effects are related to this inhibition of DNA 
      synthesis, cytotoxicity and cell cycle progression in human lymphoblastoid cell 
      lines, proficient or deficient in salvage nucleotide synthesis pathways, were 
      determined after exposures to 3AB. In addition, changes in cell cycle progression 
      were followed after treatment with 3-aminobenzoic acid (ABA), a nonactive analog 
      of 3AB, and 5-methylnicotinamide (5MN), a less potent inhibitor of 
      poly(ADP-ribose) synthesis. All three lines responded similarly to the different 
      treatments. Cells deficient in salvage nucleotide synthesis pathways were no more 
      sensitive to the cytotoxic or cell cycle effects of 3AB, ABA or 5MN. Thus, 
      effects on nucleotide synthesis are not responsible for either cytotoxicity or 
      the retarded cell growth found in human lymphoblastoid cells exposed to 
      inhibitors of poly(ADP-ribose) synthesis.
FAU - Schwartz, J L
AU  - Schwartz JL
FAU - Weichselbaum, R R
AU  - Weichselbaum RR
LA  - eng
GR  - CA 29883/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Aminobenzoates)
RN  - 0 (Benzamides)
RN  - 0 (Hypoxanthines)
RN  - 0 (Nucleotides)
RN  - 0 (Poly(ADP-ribose) Polymerase Inhibitors)
RN  - 0 (meta-Aminobenzoates)
RN  - 0U46U6E8UK (NAD)
RN  - 25X51I8RD4 (Niacinamide)
RN  - 2TN51YD919 (Hypoxanthine)
RN  - 70-57-5 (5-methylnicotinamide)
RN  - 8J365YF1YH (3-aminobenzamide)
RN  - EC 3.2.2.5 (NAD+ Nucleosidase)
RN  - G2X3B3O37U (3-aminobenzoic acid)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Aminobenzoates/pharmacology
MH  - Benzamides/*pharmacology
MH  - Cell Cycle/*drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Humans
MH  - Hypoxanthine
MH  - Hypoxanthines/metabolism
MH  - Lymphocytes/cytology/*drug effects/metabolism
MH  - NAD/metabolism
MH  - NAD+ Nucleosidase/*antagonists & inhibitors
MH  - Niacinamide/analogs & derivatives/pharmacology
MH  - Nucleotides/*biosynthesis/blood
MH  - *Poly(ADP-ribose) Polymerase Inhibitors
MH  - Thymidine/metabolism
MH  - meta-Aminobenzoates
EDAT- 1985/03/01 00:00
MHDA- 1985/03/01 00:01
CRDT- 1985/03/01 00:00
PHST- 1985/03/01 00:00 [pubmed]
PHST- 1985/03/01 00:01 [medline]
PHST- 1985/03/01 00:00 [entrez]
AID - 0014-4827(85)90169-7 [pii]
AID - 10.1016/0014-4827(85)90169-7 [doi]
PST - ppublish
SO  - Exp Cell Res. 1985 Mar;157(1):271-5. doi: 10.1016/0014-4827(85)90169-7.