PMID- 29843929
OWN - NLM
STAT- MEDLINE
DCOM- 20190610
LR  - 20190619
IS  - 1879-1476 (Electronic)
IS  - 0385-8146 (Linking)
VI  - 46
IP  - 1
DP  - 2019 Feb
TI  - MicroRNA-4497 functions as a tumor suppressor in laryngeal squamous cell 
      carcinoma via negatively modulation the GBX2.
PG  - 106-113
LID - S0385-8146(18)30168-8 [pii]
LID - 10.1016/j.anl.2018.05.005 [doi]
AB  - OBJECTIVE: MicroRNAs (miRNAs) are aberrantly expressed in various tumors and play 
      a critical role in the progression and development of tumors. However, there is 
      little information about the role of miR-4497 in laryngeal squamous cell 
      carcinoma (LSCC). The aim of this study is to investigate the role of miR-4497 in 
      LSCC. METHODS: MiR-4497 expression in tumor tissues and adjacent normal tissues 
      was measured by RT-PCR. The effects of miR-4497 on cell viability and apoptosis 
      were evaluated by the MTT assay, Flow cytometry and caspase-3 activity assay. 
      Western blot analysis was used to measure the expression of various proteins. 
      Bioinformatic analysis and luciferase reporter assay were applied to investigate 
      the relationship between miR-4497 and GBX2. RESULTS: We found that miR-4497 
      expression was downregulated in LSCC tumor tissues and cell lines compared to the 
      normal counterparts. Overexpression of miR-4497 inhibits the proliferation and 
      induces apoptosis of LSCC cells accompanied by the down-regulation of 
      anti-apoptotic Bcl-2 proteins. Mechanisms investigation revealed that GBX2 is a 
      direct target of miR-4497. miR-4497 expression was inversely correlated with GBX2 
      expression in LSCC tissues. Moreover, overexpression of miR-4497 leads to the 
      activation of ERK, JNK but not p38. Inhibition of ERK by specific inhibitor 
      SCH772984 could interfere the apoptosis induced by overexpression of miR-4497. 
      CONCLUSION: Therefore, our results indicate that miR-4497 may play a suppressive 
      role in LSCC by targeting GBX2, which offer new insights into the tumorigenesis 
      of LSCC.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Chen, Xudong
AU  - Chen X
AD  - Department of Otolaryngology, The First Hospital of Ningbo, Ningbo, People's 
      Republic of China.
FAU - Zhang, Litao
AU  - Zhang L
AD  - Department of Otolaryngology, The First Hospital of Ningbo, Ningbo, People's 
      Republic of China.
FAU - Tang, Shixiong
AU  - Tang S
AD  - Department of Otolaryngology, The First Hospital of Ningbo, Ningbo, People's 
      Republic of China. Electronic address: wizardcxd@163.com.
LA  - eng
PT  - Journal Article
DEP - 20180526
PL  - Netherlands
TA  - Auris Nasus Larynx
JT  - Auris, nasus, larynx
JID - 7708170
RN  - 0 (BCL2 protein, human)
RN  - 0 (BCL2L1 protein, human)
RN  - 0 (GBX2 protein, human)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MCL1 protein, human)
RN  - 0 (MIRN4497 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Myeloid Cell Leukemia Sequence 1 Protein)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (bcl-X Protein)
SB  - IM
MH  - Apoptosis/genetics
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Cell Survival/genetics
MH  - Colony Count, Microbial
MH  - Flow Cytometry
MH  - Homeodomain Proteins/*genetics/metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism
MH  - MAP Kinase Signaling System
MH  - MicroRNAs/genetics/*physiology
MH  - Myeloid Cell Leukemia Sequence 1 Protein/genetics/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics/metabolism
MH  - bcl-X Protein/genetics/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - GBX2
OT  - Laryngeal squamous cell carcinoma (LSCC)
OT  - MAPKs
OT  - MiR-4497
EDAT- 2018/05/31 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/05/31 06:00
PHST- 2018/02/12 00:00 [received]
PHST- 2018/04/08 00:00 [revised]
PHST- 2018/05/08 00:00 [accepted]
PHST- 2018/05/31 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2018/05/31 06:00 [entrez]
AID - S0385-8146(18)30168-8 [pii]
AID - 10.1016/j.anl.2018.05.005 [doi]
PST - ppublish
SO  - Auris Nasus Larynx. 2019 Feb;46(1):106-113. doi: 10.1016/j.anl.2018.05.005. Epub 
      2018 May 26.