PMID- 29844815
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 6
DP  - 2018 Jun
TI  - Identification of crucial genes associated with esophageal squamous cell 
      carcinoma by gene expression profile analysis.
PG  - 8983-8990
LID - 10.3892/ol.2018.8464 [doi]
AB  - To uncover the genes associated with the development of esophageal squamous cell 
      carcinoma (ESCC), an ESCC microarray dataset was used to identify genes 
      differentially expressed between ESCC and normal control tissues. The dataset 
      GSE17351 was downloaded from the Gene Expression Omnibus, containing 5 tumor 
      esophageal mucosa samples and 5 adjacent normal esophageal mucosa samples from 5 
      male patients with ESCC. The differentially expressed genes (DEGs) were 
      identified using the Linear Models for Microarray Data R package. Then, a 
      co-expression network was constructed using the Weighted Correlation Network 
      Analysis (WGCNA) package, and co-expression network modules were obtained with a 
      hierarchical clustering algorithm. Additionally, functional enrichment analyses 
      for DEGs in the top 2 modules with the highest significance were respectively 
      conducted using the WGCNA package and the cluster Profiler package. In total, 487 
      upregulated and 468 downregulated DEGs were identified. A total of 24 modules 
      were obtained from the co-expression network, and the top 2 modules with the 
      highest significance, designated as 'blue4' and 'magenta', were further analyzed. 
      In the module blue4, DEGs were significantly enriched in a number of Gene 
      Ontology terms, including 'spindle organization' [e.g., ubiquitin conjugating 
      enzyme E2 C (UBE2C) and SAC3 domain containing 1] and 'cell cycle process' [e.g., 
      UBE2C, minichromosome maintenance complex component 6 (MCM6) and cell division 
      cycle 20 (CDC20)]. Furthermore, a number of DEGs (e.g., UBE2C, CDC20 and MCM6) 
      were enriched in the 'cell cycle' and 'ubiquitin mediated proteolysis' pathways. 
      In the module 'magenta', a number of DEGs [e.g., transferrin receptor (TFRC) and 
      TEA domain transcription factor 4 (TEAD4)] were enriched in the primary metabolic 
      process and intracellular membrane-bounded organelle. Additionally, 308 
      upregulated genes and 215 downregulated genes were differentially expressed in 
      the same pattern in another dataset, GSE20347, including UBE2C, CDC20, MCM6, 
      TFRC, TEAD4, protein phosphatase 1 regulatory subunit 3C and MAL, T-cell 
      differentiation protein. These DEGs may function in the development of ESCC.
FAU - Wang, Xuehai
AU  - Wang X
AD  - Department of Thoracic Surgery, Sichuan Academy of Medical Sciences and Sichuan 
      Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
FAU - Li, Gang
AU  - Li G
AD  - Department of Thoracic Surgery, Sichuan Academy of Medical Sciences and Sichuan 
      Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
FAU - Luo, Qingsong
AU  - Luo Q
AD  - Department of Thoracic Surgery, Sichuan Academy of Medical Sciences and Sichuan 
      Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
FAU - Gan, Chongzhi
AU  - Gan C
AD  - Department of Thoracic Surgery, Sichuan Academy of Medical Sciences and Sichuan 
      Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180411
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5958829
OTO - NOTNLM
OT  - co-expression network
OT  - differentially expressed gene
OT  - esophageal squamous cell carcinoma
OT  - module
EDAT- 2018/05/31 06:00
MHDA- 2018/05/31 06:01
PMCR- 2018/04/11
CRDT- 2018/05/31 06:00
PHST- 2016/08/05 00:00 [received]
PHST- 2017/11/16 00:00 [accepted]
PHST- 2018/05/31 06:00 [entrez]
PHST- 2018/05/31 06:00 [pubmed]
PHST- 2018/05/31 06:01 [medline]
PHST- 2018/04/11 00:00 [pmc-release]
AID - OL-0-0-8464 [pii]
AID - 10.3892/ol.2018.8464 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 Jun;15(6):8983-8990. doi: 10.3892/ol.2018.8464. Epub 2018 Apr 
      11.