PMID- 29845350 OWN - NLM STAT- MEDLINE DCOM- 20180913 LR - 20180913 IS - 1432-8798 (Electronic) IS - 0304-8608 (Linking) VI - 163 IP - 10 DP - 2018 Oct TI - Phylogenetic analysis of porcine reproductive and respiratory syndrome virus isolates from Northern Ireland. PG - 2799-2804 LID - 10.1007/s00705-018-3886-7 [doi] AB - To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nucleotide level and 84.1-93.5% identity at the protein level. Phylogenetic analysis confirmed that all nine isolates belonged to the European (type 1) genotype and formed a cluster within the subtype 1 subgroup. This study provides the first report on PRRSV isolate diversity in Northern Ireland. FAU - Smith, Natalie AU - Smith N AD - Veterinary Science Division, Agri-Food and Biosciences Institute, Stormont, Belfast, BT4 3SD, UK. AD - Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Medical Biology Centre, Queen's University Belfast, Belfast, BT9 7BL, UK. FAU - Power, Ultan F AU - Power UF AD - Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Medical Biology Centre, Queen's University Belfast, Belfast, BT9 7BL, UK. FAU - McKillen, John AU - McKillen J AD - Veterinary Science Division, Agri-Food and Biosciences Institute, Stormont, Belfast, BT4 3SD, UK. john.mckillen@afbini.gov.uk. LA - eng PT - Journal Article DEP - 20180529 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 RN - 0 (Viral Envelope Proteins) SB - IM MH - Animals MH - Genetic Variation MH - Genotype MH - Northern Ireland MH - *Phylogeny MH - Porcine Reproductive and Respiratory Syndrome/*virology MH - Porcine respiratory and reproductive syndrome virus/*classification/genetics/*isolation & purification MH - Swine MH - Viral Envelope Proteins/genetics EDAT- 2018/05/31 06:00 MHDA- 2018/09/14 06:00 CRDT- 2018/05/31 06:00 PHST- 2018/02/13 00:00 [received] PHST- 2018/05/17 00:00 [accepted] PHST- 2018/05/31 06:00 [pubmed] PHST- 2018/09/14 06:00 [medline] PHST- 2018/05/31 06:00 [entrez] AID - 10.1007/s00705-018-3886-7 [pii] AID - 10.1007/s00705-018-3886-7 [doi] PST - ppublish SO - Arch Virol. 2018 Oct;163(10):2799-2804. doi: 10.1007/s00705-018-3886-7. Epub 2018 May 29.