PMID- 29845664
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1365-3164 (Electronic)
IS  - 0959-4493 (Linking)
DP  - 2018 May 29
TI  - Minimum inhibitory concentration and killing properties of rifampicin against 
      canine Staphylococcus pseudintermedius isolates from dogs in the southeast USA.
LID - 10.1111/vde.12653 [doi]
AB  - BACKGROUND: Meticillin-resistant (MR) staphylococcal pyoderma in dogs has led to 
      increased use of alternate antibiotics such as rifampicin (RFP). However, little 
      information exists regarding its pharmacodynamics in MR Staphylococcus 
      pseudintermedius. HYPOTHESIS/OBJECTIVES: To determine the minimum inhibitory 
      concentration (MIC) and killing properties of RFP for canine Staphylococcus 
      pseudintermedius isolates. METHODS: The MIC of RFP was determined using the 
      ETEST((R)) for 50 meticillin-susceptible (MS) and 50 MR S. pseudintermedius 
      isolates collected from dogs. From these isolates, two MS isolates (RFP MIC of 
      0.003 and 0.008 mug/mL, respectively) and two MR isolates (RFP MIC of 0.003 and 
      0.012 mug/mL, respectively) were subjected to time-kill studies. Mueller-Hinton 
      broth was supplemented with RFP at 0, 0.5, 1, 2, 4, 8, 16 and 32 times the MIC 
      for 0, 2, 4, 10, 16 and 24 h. The number of viable colony forming units in each 
      sample was determined using a commercial luciferase assay kit. RESULTS: The 
      MIC(50) and MIC(90) were the same for MS and MR isolates, at 0.004 mug/mL and 
      0.008 mug/mL, respectively. Rifampicin kill curves were not indicative of 
      concentration-dependency, suggesting time-dependent activity. Two isolates (MS 
      0.003 and 0.008 mug/mL) exhibited bacteriostatic activity, whereas two others (MR 
      0.003 and 0.012 mug/mL) exhibited bactericidal activity. CONCLUSIONS AND CLINICAL 
      IMPORTANCE: This study demonstrated that MS and MR S. pseudintermedius isolates 
      were equally susceptible to rifampicin and that dosing intervals should be 
      designed for time-dependent efficacy. These data can support pharmacokinetic 
      studies of RFP in dogs with susceptible infections caused by S. pseudintermedius.
CI  - (c) 2018 ESVD and ACVD.
FAU - Ho, Karen K
AU  - Ho KK
AUID- ORCID: 0000-0003-4862-9177
AD  - Department of Clinical Sciences, College of Veterinary Medicine, Auburn 
      University, 1220 Wire Road, Auburn, AL, 36849, USA.
FAU - Conley, Austin C
AU  - Conley AC
AD  - Department of Anatomy, Physiology, and Pharmacology, College of Veterinary 
      Medicine, Auburn University, 109 Greene Hall, Auburn, AL, 36849, USA.
FAU - Kennis, Robert A
AU  - Kennis RA
AD  - Department of Clinical Sciences, College of Veterinary Medicine, Auburn 
      University, 1220 Wire Road, Auburn, AL, 36849, USA.
FAU - Hathcock, Terri L
AU  - Hathcock TL
AD  - Department of Pathobiology, College of Veterinary Medicine, Auburn University, 
      264 Greene Hall, Auburn, AL, 36849, USA.
FAU - Boothe, Dawn M
AU  - Boothe DM
AD  - Department of Anatomy, Physiology, and Pharmacology, College of Veterinary 
      Medicine, Auburn University, 109 Greene Hall, Auburn, AL, 36849, USA.
FAU - White, Amelia G
AU  - White AG
AD  - Department of Clinical Sciences, College of Veterinary Medicine, Auburn 
      University, 1220 Wire Road, Auburn, AL, 36849, USA.
LA  - eng
PT  - Journal Article
DEP - 20180529
PL  - England
TA  - Vet Dermatol
JT  - Veterinary dermatology
JID - 9426187
EDAT- 2018/05/31 06:00
MHDA- 2018/05/31 06:00
CRDT- 2018/05/31 06:00
PHST- 2018/04/12 00:00 [accepted]
PHST- 2018/05/31 06:00 [entrez]
PHST- 2018/05/31 06:00 [pubmed]
PHST- 2018/05/31 06:00 [medline]
AID - 10.1111/vde.12653 [doi]
PST - aheadofprint
SO  - Vet Dermatol. 2018 May 29. doi: 10.1111/vde.12653.