PMID- 29846983
OWN - NLM
STAT- MEDLINE
DCOM- 20191104
LR  - 20200306
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 47
IP  - 12
DP  - 2018 Jun
TI  - Lasting changes induced by mild alcohol exposure during embryonic development in 
      BDNF, NCAM and synaptophysin-positive neurons quantified in adult zebrafish.
PG  - 1457-1473
LID - 10.1111/ejn.13975 [doi]
AB  - Fetal alcohol spectrum disorder is one of the leading causes of mental health 
      issues worldwide. Analysis of zebrafish exposed to alcohol during embryonic 
      development confirmed that even low concentrations of alcohol for a short period 
      of time may have lasting behavioral consequences at the adult or old age. The 
      mechanism of this alteration has not been studied. Here, we immersed zebrafish 
      embryos into 1% alcohol solution (vol/vol%) at 24 hr post-fertilization (hpf) for 
      2 hr and analyzed potential changes using immunohistochemistry. We measured the 
      number of BDNF (brain-derived neurotrophic factor) and NCAM (neuronal cell 
      adhesion molecule)-positive neurons and the intensity of synaptophysin staining 
      in eight brain regions: lateral zone of the dorsal telencephalic area, medial 
      zone of the dorsal telencephalic area, dorsal nucleus of the ventral 
      telencephalic area, ventral nucleus of the ventral telencephalic area, 
      parvocellular preoptic nucleus, ventral habenular nucleus, corpus cerebella and 
      inferior reticular formation. We found embryonic alcohol exposure to 
      significantly reduce the number of BDNF- and NCAM-positive cells in all brain 
      areas studied as compared to control. We also found alcohol to significantly 
      reduce the intensity of synaptophysin staining in all brain areas except the 
      cerebellum and preoptic area. These neuroanatomical changes correlated with 
      previously demonstrated reduction of social behavior in embryonic alcohol-exposed 
      zebrafish, raising the possibility of a causal link. Given the evolutionary 
      conservation across fish and mammals, we emphasize the implication of our current 
      study for human health: even small amount of alcohol consumption may be unsafe 
      during pregnancy.
CI  - (c) 2018 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.
FAU - Mahabir, Samantha
AU  - Mahabir S
AD  - Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada.
FAU - Chatterjee, Dipashree
AU  - Chatterjee D
AD  - Department of Psychology, University of Toronto Mississauga, Mississauga, ON, 
      Canada.
FAU - Misquitta, Keith
AU  - Misquitta K
AD  - Department of Psychology, University of Toronto Mississauga, Mississauga, ON, 
      Canada.
FAU - Chatterjee, Diptendu
AU  - Chatterjee D
AD  - Department of Psychology, University of Toronto Mississauga, Mississauga, ON, 
      Canada.
FAU - Gerlai, Robert
AU  - Gerlai R
AUID- ORCID: 0000-0001-5514-3859
AD  - Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada.
AD  - Department of Psychology, University of Toronto Mississauga, Mississauga, ON, 
      Canada.
LA  - eng
GR  - R01 AA014357/AA/NIAAA NIH HHS/United States
GR  - R01 AA015325/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20180615
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (BDNF protein, zebrafish)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Central Nervous System Depressants)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Synaptophysin)
RN  - 0 (Teratogens)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (ncam1a protein, zebrafish)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Brain/cytology/*drug effects
MH  - *Brain-Derived Neurotrophic Factor
MH  - Central Nervous System Depressants/*adverse effects
MH  - Disease Models, Animal
MH  - Embryo, Nonmammalian/cytology/*drug effects
MH  - Embryonic Development/*drug effects
MH  - Ethanol/*adverse effects
MH  - *Fetal Alcohol Spectrum Disorders
MH  - *Neural Cell Adhesion Molecules
MH  - Neuronal Plasticity/*drug effects
MH  - Neurons/cytology/*drug effects
MH  - *Synaptophysin
MH  - Teratogens/*pharmacology
MH  - Zebrafish
MH  - *Zebrafish Proteins
PMC - PMC6030466
MID - NIHMS971442
OTO - NOTNLM
OT  - brain plasticity
OT  - fetal alcohol spectrum disorder
OT  - neuronal protein
OT  - prenatal
OT  - teratogen
COIS- Competing Interests No conflicts of interests.
EDAT- 2018/05/31 06:00
MHDA- 2019/11/05 06:00
PMCR- 2019/06/15
CRDT- 2018/05/31 06:00
PHST- 2017/08/03 00:00 [received]
PHST- 2018/05/15 00:00 [revised]
PHST- 2018/05/18 00:00 [accepted]
PHST- 2018/05/31 06:00 [pubmed]
PHST- 2019/11/05 06:00 [medline]
PHST- 2018/05/31 06:00 [entrez]
PHST- 2019/06/15 00:00 [pmc-release]
AID - 10.1111/ejn.13975 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2018 Jun;47(12):1457-1473. doi: 10.1111/ejn.13975. Epub 2018 Jun 
      15.