PMID- 29847601
OWN - NLM
STAT- MEDLINE
DCOM- 20180806
LR  - 20240326
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 5
DP  - 2018
TI  - Identification of immunogenic proteins and evaluation of recombinant PDHA1 and 
      GAPDH as potential vaccine candidates against Streptococcus iniae infection in 
      flounder (Paralichthys olivaceus).
PG  - e0195450
LID - 10.1371/journal.pone.0195450 [doi]
LID - e0195450
AB  - Streptococcus iniae is a major Gram-positive pathogen that causes invasive 
      disease in fish worldwide. In this study, in order to identify immunogenic 
      proteins for developing highly effective vaccine against S. iniae, whole-cell 
      lysate proteins of S. iniae were analyzed by western blotting using flounder 
      anti-S. iniae antibodies, and two positive protein bands of molecular weight 37 
      kDa and 40 kDa were screened, which were identified as pyruvate dehydrogenase E1 
      subunit alpha (PDHA1), BMP family ABC transporter substrate-binding protein (BMP) 
      and L-lactate dehydrogenase (LDH), as well as ornithine carbamoyltransferase 
      (OCT), lactate oxidas (LOx) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) 
      by mass spectrometry. Subsequently, the six recombinant proteins were produced 
      and used to immunize healthy flounder, and the relative percent survival (RPS) 
      value was 72.73%, 27.27%, 36.36%, 9.09%, 36.36% and 63.64% respectively after 
      intraperitoneal challenge with live S. iniae, revealing that rPDHA1 and rGAPDH 
      produced higher relative percent survival than formalin-killed S. iniae (36.36%). 
      To further investigate the protective efficacy of rPDHA1 and rGAPDH, the 
      proliferation of surface membrane immunoglobulin-positive (sIg+) lymphocytes in 
      peripheral blood leucocytes, the total serum IgM, specific IgM against S. iniae 
      and RPS were detected. The results showed that rPDHA1, rGAPDH and formalin-killed 
      S. iniae significantly induced the proliferation of sIg+ lymphocytes, the 
      production of total serum IgM and specific IgM as compared with the control 
      group, and rGAPDH and rPDHA1 provide higher RPS (62.5% and 75%, respectively) 
      again. These results demonstrated that rPDHA1 and rGAPDH are promising vaccine 
      candidates against S. iniae infection in flounder.
FAU - Sheng, Xiuzhen
AU  - Sheng X
AD  - Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, P. R. China.
FAU - Liu, Min
AU  - Liu M
AD  - Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, P. R. China.
FAU - Liu, Haibo
AU  - Liu H
AD  - Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, P. R. China.
FAU - Tang, Xiaoqian
AU  - Tang X
AD  - Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, P. R. China.
FAU - Xing, Jing
AU  - Xing J
AD  - Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, P. R. China.
FAU - Zhan, Wenbin
AU  - Zhan W
AUID- ORCID: 0000-0002-5672-5137
AD  - Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean 
      University of China, Qingdao, P. R. China.
AD  - Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology, Qingdao, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180530
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Streptococcal Vaccines)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 1.2.4.1 (Pyruvate Dehydrogenase (Lipoamide))
RN  - EC 1.2.4.1 (pyruvate dehydrogenase E1alpha subunit)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/immunology
MH  - Fish Diseases/*immunology/prevention & control
MH  - Flounder/*immunology/microbiology
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*immunology
MH  - Pyruvate Dehydrogenase (Lipoamide)/*immunology
MH  - Recombinant Proteins/*immunology
MH  - Streptococcal Infections/*immunology/microbiology/prevention & control
MH  - Streptococcal Vaccines/administration & dosage/immunology
MH  - Streptococcus iniae/*immunology
MH  - Vaccination
PMC - PMC5976140
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/05/31 06:00
MHDA- 2018/08/07 06:00
PMCR- 2018/05/30
CRDT- 2018/05/31 06:00
PHST- 2018/01/24 00:00 [received]
PHST- 2018/03/22 00:00 [accepted]
PHST- 2018/05/31 06:00 [entrez]
PHST- 2018/05/31 06:00 [pubmed]
PHST- 2018/08/07 06:00 [medline]
PHST- 2018/05/30 00:00 [pmc-release]
AID - PONE-D-18-02505 [pii]
AID - 10.1371/journal.pone.0195450 [doi]
PST - epublish
SO  - PLoS One. 2018 May 30;13(5):e0195450. doi: 10.1371/journal.pone.0195450. 
      eCollection 2018.