PMID- 29847822
OWN - NLM
STAT- MEDLINE
DCOM- 20181109
LR  - 20181109
IS  - 1660-5535 (Electronic)
IS  - 1660-5527 (Linking)
VI  - 31
IP  - 5
DP  - 2018
TI  - Basic Fibroblast Growth Factor Influences Epidermal Homeostasis of Living Skin 
      Equivalents through Affecting Fibroblast Phenotypes and Functions.
PG  - 229-237
LID - 10.1159/000488992 [doi]
AB  - AIMS: To elucidate the possible mechanisms of how basic fibroblast growth factor 
      (bFGF) influences epidermal homeostasis in a living skin equivalent (LSE) model. 
      METHODS: Several wound healing-related growth factors were analyzed at protein 
      and mRNA levels for dermal fibroblasts of induced alpha-smooth muscle actin 
      (alpha-SMA)-positive or alpha-SMA-negative phenotypes. During culturing an LSE model by 
      seeding normal human keratinocytes on a fibroblast-populated type I collagen gel, 
      bFGF or neutralizing antibody for keratinocyte growth factor (KGF) was added to 
      investigate its effects on fibroblast phenotypes and, subsequently, epidermal 
      homeostasis by histology and immunohistochemistry. RESULTS: The alpha-SMA-positive 
      phenotype of fibroblasts induced by transforming growth factor beta-1 (TGF-beta1) 
      markedly suppressed the expression of KGF and hepatocyte growth factor (HGF), and 
      slightly upregulated vascular endothelial growth factor (VEGF) and TGF-beta1 at mRNA 
      and protein levels, compared with alpha-SMA-negative fibroblasts treated with bFGF. 
      alpha-SMA expression of fibroblasts at the epidermal-mesenchymal junction of the LSEs 
      was suppressed by the addition of bFGF, and a better-differentiated epidermis was 
      presented. The abrogation of KGF from fibroblasts by the addition of the KGF 
      neutralizing antibody disenabled the LSE culturing system to develop an 
      epidermis. CONCLUSIONS: bFGF, through affecting the phenotypes and functions of 
      fibroblasts, especially KGF expression, influenced epidermal homeostasis in an 
      LSE model.
CI  - (c) 2018 S. Karger AG, Basel.
FAU - Yang, Lujun
AU  - Yang L
AD  - Department of Burns and Plastic Surgery, Second Affiliated Hospital, Shantou 
      University Medical College, Shantou, China.
AD  - Research Center for Translational Medicine, Shantou University Medical College, 
      Shantou, China.
FAU - Zhang, Dangui
AU  - Zhang D
AD  - Research Center for Translational Medicine, Shantou University Medical College, 
      Shantou, China.
FAU - Wu, Hongjuan
AU  - Wu H
AD  - Department of Burns and Plastic Surgery, Shaanxi Provincial People's Hospital, 
      Xi'an, China.
FAU - Xie, Sitian
AU  - Xie S
AD  - Department of Burns and Plastic Surgery, Second Affiliated Hospital, Shantou 
      University Medical College, Shantou, China.
FAU - Zhang, Mingjun
AU  - Zhang M
AD  - Department of Burns and Plastic Surgery, Second Affiliated Hospital, Shantou 
      University Medical College, Shantou, China.
FAU - Zhang, Bingna
AU  - Zhang B
AD  - Research Center for Translational Medicine, Shantou University Medical College, 
      Shantou, China.
FAU - Tang, Shijie
AU  - Tang S
AD  - Department of Burns and Plastic Surgery, Second Affiliated Hospital, Shantou 
      University Medical College, Shantou, China.
LA  - eng
PT  - Journal Article
DEP - 20180530
PL  - Switzerland
TA  - Skin Pharmacol Physiol
JT  - Skin pharmacology and physiology
JID - 101188418
RN  - 0 (Actins)
RN  - 0 (HGF protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Actins/metabolism
MH  - Cells, Cultured
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Fibroblast Growth Factor 7/genetics/*metabolism
MH  - Fibroblasts/*drug effects/metabolism
MH  - Hepatocyte Growth Factor/genetics/metabolism
MH  - Homeostasis/drug effects
MH  - Humans
MH  - Keratinocytes/metabolism
MH  - Phenotype
MH  - Skin/metabolism
MH  - Transforming Growth Factor beta1/genetics/metabolism
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
OTO - NOTNLM
OT  - Basic fibroblast growth factor
OT  - Epidermal homeostasis
OT  - Fibroblast phenotype
OT  - Keratinocyte growth factor
OT  - Living skin equivalent
EDAT- 2018/05/31 06:00
MHDA- 2018/11/10 06:00
CRDT- 2018/05/31 06:00
PHST- 2017/08/15 00:00 [received]
PHST- 2018/04/04 00:00 [accepted]
PHST- 2018/05/31 06:00 [pubmed]
PHST- 2018/11/10 06:00 [medline]
PHST- 2018/05/31 06:00 [entrez]
AID - 000488992 [pii]
AID - 10.1159/000488992 [doi]
PST - ppublish
SO  - Skin Pharmacol Physiol. 2018;31(5):229-237. doi: 10.1159/000488992. Epub 2018 May 
      30.