PMID- 29849572 OWN - NLM STAT- MEDLINE DCOM- 20181126 LR - 20181126 IS - 1687-5443 (Electronic) IS - 2090-5904 (Print) IS - 1687-5443 (Linking) VI - 2018 DP - 2018 TI - Peripheral Nerve Injury-Induced Astrocyte Activation in Spinal Ventral Horn Contributes to Nerve Regeneration. PG - 8561704 LID - 10.1155/2018/8561704 [doi] LID - 8561704 AB - Accumulating evidences suggest that peripheral nerve injury (PNI) may initiate astrocytic responses in the central nervous system (CNS). However, the response of astrocytes in the spinal ventral horn and its potential role in nerve regeneration after PNI remain unclear. Herein, we firstly illustrated that astrocytes in the spinal ventral horn were dramatically activated in the early stage following sciatic nerve injury, and these profiles were eliminated in the chronic stage. Additionally, we found that the expression of neurotrophins, including brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and neurotrophin-3 (NT-3), also accompanied with astrocyte activation. In comparison with the irreversible transected subjects, astrocyte activation and the neurotrophic upregulation in the early stage were more drastic in case the transected nerve was rebridged immediately after injury. Furthermore, administering fluorocitrate to inhibit astrocyte activation resulted in decreased neurotrophin expression in the spinal ventral horn and delayed axonal regeneration in the nerve as well as motor function recovery. Overall, the present study indicates that peripheral nerve injury can initiate astrocyte activation accompanied with neurotrophin upregulation in the spinal ventral horn. The above responses mainly occur in the early stage of PNI and may contribute to nerve regeneration and motor function recovery. FAU - Qian, Changhui AU - Qian C AUID- ORCID: 0000-0003-3394-0456 AD - Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China. AD - Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. AD - Department of Histology and Embryology, Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China. FAU - Tan, Dandan AU - Tan D AUID- ORCID: 0000-0001-5216-6393 AD - Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China. AD - Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. FAU - Wang, Xianghai AU - Wang X AD - Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China. AD - Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. FAU - Li, Lixia AU - Li L AUID- ORCID: 0000-0002-9928-2882 AD - Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China. AD - Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. FAU - Wen, Jinkun AU - Wen J AUID- ORCID: 0000-0002-3615-8147 AD - Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China. AD - Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. FAU - Pan, Mengjie AU - Pan M AD - Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China. AD - Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. FAU - Li, Yuanyuan AU - Li Y AD - Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China. FAU - Wu, Wutian AU - Wu W AD - Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong. AD - State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Pokfulam, Hong Kong. AD - Joint Laboratory of Jinan University and The University of Hong Kong, GHM Institute of CNS Regeneration, Jinan University, Guangzhou 510632, China. FAU - Guo, Jiasong AU - Guo J AUID- ORCID: 0000-0002-7885-2903 AD - Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou 510515, China. AD - Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. AD - Institute of Bone Biology, Academy of Orthopedics, Guangzhou, Guangdong Province 510665, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180403 PL - United States TA - Neural Plast JT - Neural plasticity JID - 100883417 RN - 0 (Nerve Growth Factors) SB - IM MH - Animals MH - Astrocytes/*metabolism MH - Female MH - Nerve Growth Factors/metabolism MH - *Nerve Regeneration MH - Peripheral Nerve Injuries/complications/*metabolism MH - Rats, Sprague-Dawley MH - Sciatic Nerve/injuries MH - Spinal Cord Injuries/complications/*metabolism MH - Spinal Cord Ventral Horn/*metabolism/physiopathology PMC - PMC5903197 EDAT- 2018/06/01 06:00 MHDA- 2018/11/27 06:00 PMCR- 2018/04/03 CRDT- 2018/06/01 06:00 PHST- 2017/11/30 00:00 [received] PHST- 2018/02/03 00:00 [revised] PHST- 2018/02/20 00:00 [accepted] PHST- 2018/06/01 06:00 [entrez] PHST- 2018/06/01 06:00 [pubmed] PHST- 2018/11/27 06:00 [medline] PHST- 2018/04/03 00:00 [pmc-release] AID - 10.1155/2018/8561704 [doi] PST - epublish SO - Neural Plast. 2018 Apr 3;2018:8561704. doi: 10.1155/2018/8561704. eCollection 2018.