PMID- 29856997
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20181105
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 502
IP  - 4
DP  - 2018 Aug 25
TI  - Anti-viral immune response in the lung and thymus: Molecular characterization and 
      expression analysis of immunoproteasome subunits LMP2, LMP7 and MECL-1 in pigs.
PG  - 472-478
LID - S0006-291X(18)31279-8 [pii]
LID - 10.1016/j.bbrc.2018.05.190 [doi]
AB  - Both the lung and the thymus are vital target organ for pathogens including 
      viruses. The immunoproteasome (i-proteasome) enhances antigen presentation for 
      MHC class I molecules to activate CD8+T lymphocyte. These facilitate antiviral 
      adaptive immune response. Our previous study found that, expression of 
      i-proteasome subunits in porcine lung was altered during normal and inflammatory 
      conditions. To date, the expression of i-proteasome subunits in porcine thymus to 
      viruses has not been investigated. In the present study, LMP2, LMP7, and MECL-1 
      were cloned, identified and their sequences encoded predicted proteins of 216, 
      275, and 278 amino acids, respectively. Expression of LMP2, LMP7, and MECL-1, in 
      the cytoplasm and nucleus, was markedly altered in the porcine reproductive and 
      respiratory syndrome virus (PRRSV)-infected lung and thymus. And dendritic cells 
      and epithelial cells readily expressed the i-proteasome subunit LMP2 in the 
      thymus of PRRSV-infected pigs compared to that in mock-infected pigs. 
      Additionally, the in vitro stimulation of a PAM cell line with PolyI:C for 12 and 
      24 h resulted in increased LMP2, LMP7, and MECL-1 expression. These results 
      suggest a central role for these complexes in the activation of an antiviral 
      immune response in pigs. A better understanding of the role of the i-proteasome 
      in different cell types, tissues, and hosts could improve vaccine design and 
      facilitate the development of effective treatment strategies for viral 
      infections.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Liu, Qiang
AU  - Liu Q
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: liuqiang@caas.cn.
FAU - Hu, Wei
AU  - Hu W
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: huwei05190806y@163.com.
FAU - Zhang, Yong-Li
AU  - Zhang YL
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: 13949054838@126.com.
FAU - Hu, Shou-Ping
AU  - Hu SP
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: hshp02@126.com.
FAU - Zhang, Zhuo
AU  - Zhang Z
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: clearzhang1006@sina.com.
FAU - He, Xi-Jun
AU  - He XJ
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: hexijun@caas.cn.
FAU - Cai, Xue-Hui
AU  - Cai XH
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: aci139@sina.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180601
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (RNA, Messenger)
RN  - 144416-78-4 (LMP-2 protein)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (LMP7 protein)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen Presentation
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Line
MH  - Cysteine Endopeptidases/chemistry/genetics/immunology
MH  - Lung/*immunology/virology
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/immunology
MH  - Porcine respiratory and reproductive syndrome virus/immunology/pathogenicity
MH  - Proteasome Endopeptidase Complex/chemistry/genetics/*immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Swine/genetics/*immunology/virology
MH  - Thymus Gland/*immunology/virology
OTO - NOTNLM
OT  - Immunoproteasome
OT  - LMP2
OT  - LMP7
OT  - MECL-1
OT  - Pig
OT  - Thymus
EDAT- 2018/06/02 06:00
MHDA- 2018/11/06 06:00
CRDT- 2018/06/02 06:00
PHST- 2018/05/22 00:00 [received]
PHST- 2018/05/28 00:00 [accepted]
PHST- 2018/06/02 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2018/06/02 06:00 [entrez]
AID - S0006-291X(18)31279-8 [pii]
AID - 10.1016/j.bbrc.2018.05.190 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Aug 25;502(4):472-478. doi: 
      10.1016/j.bbrc.2018.05.190. Epub 2018 Jun 1.