PMID- 29857961
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1873-569X (Electronic)
IS  - 0923-1811 (Linking)
VI  - 91
IP  - 3
DP  - 2018 Sep
TI  - The role of sex-determining region Y-box 6 in melanogenesis in alpaca 
      melanocytes.
PG  - 268-275
LID - S0923-1811(18)30233-0 [pii]
LID - 10.1016/j.jdermsci.2018.05.007 [doi]
AB  - BACKGROUND: Sex-determining region Y-box (SOX) proteins function as 
      transcriptional regulators. The derivation of melanocytes from nerve crest cells 
      has been reported to depend on SOX proteins, including SOX10 and SOX5. Whether 
      SOX6 is expressed and has a functional role in melanocytes is unknown. OBJECTIVE: 
      We aimed to study the effect of transcription factor SOX6 on melanogenesis in 
      alpaca melanocytes. METHODS: We verified the role of SOX6 in melanogenesis by 
      overexpressing and inhibiting SOX6 in melanocytes. Co-immunoprecipitation (co-IP) 
      experiments were performed to further explore the function of SOX6 in 
      melanogenesis and its mechanism of melanin production. We found that SOX6 
      interacted with cyclin-dependent kinase （CDK5）, beta-catenin, and Cyclin D1. 
      RESULTS: Bioinformatics analysis suggested that SOX6 has a phosphorylation site 
      for CDK5, which regulates melanogenesis, suggesting that SOX6 might play a role 
      in melanogenesis. Co-IP experiments indicated that SOX6 interacted with CDK5, 
      beta-catenin, and Cyclin D1. Quantitative real-time polymerase chain reaction and 
      western blot analyses of SOX6-overexpressing melanocytes revealed increased mRNA 
      and protein expression of Cyclin D1, CDK5, microphthalmia transcription factor 
      (MITF), tyrosinase (TYR), tyrosine related protein-1 (TYRP1), and 
      dopachrome-tautomerase (DCT), whereas beta-catenin levels decreased in 
      SOX6-overexpressing melanocytes. The opposite results were observed upon SOX6 
      knockdown. The melanin content was significantly increased or decreased, 
      respectively, by SOX6 overexpression or knockdown. CONCLUSION: Our results 
      suggest that SOX6 might enhance melanogenesis by binding with beta-catenin to 
      increase Cyclin D1 and MITF expression.
CI  - Copyright (c) 2018 Japanese Society for Investigative Dermatology. Published by 
      Elsevier B.V. All rights reserved.
FAU - Du, Bin
AU  - Du B
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Liu, Xuexian
AU  - Liu X
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Zhang, Pengqian
AU  - Zhang P
AD  - Department of Ecology Research, Beijing Milu Ecological Research Center, 
      Nanhaizi, Daxing District, Beijing, 102600, China.
FAU - Zhang, Junzhen
AU  - Zhang J
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Ji, Kaiyuan
AU  - Ji K
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Hu, Shuaipeng
AU  - Hu S
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Yang, Shanshan
AU  - Yang S
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Liu, Bo
AU  - Liu B
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Fan, Ruiwen
AU  - Fan R
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China. Electronic address: ruiwenfan@163.com.
LA  - eng
PT  - Journal Article
DEP - 20180523
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Melanins)
RN  - 0 (Microphthalmia-Associated Transcription Factor)
RN  - 0 (SOXD Transcription Factors)
RN  - 0 (beta Catenin)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.11.1 (Cyclin-Dependent Kinase 5)
SB  - IM
MH  - Animals
MH  - Camelids, New World
MH  - Cells, Cultured
MH  - Cyclin D1/genetics/metabolism
MH  - Cyclin-Dependent Kinase 5/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Melanins/*biosynthesis
MH  - Melanocytes/*metabolism
MH  - Microphthalmia-Associated Transcription Factor/genetics/metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - SOXD Transcription Factors/genetics/*metabolism
MH  - beta Catenin/genetics/metabolism
OTO - NOTNLM
OT  - CDK5
OT  - Cyclin D1
OT  - Melanocyte
OT  - Melanogenesis
OT  - SOX6
EDAT- 2018/06/03 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/06/03 06:00
PHST- 2018/03/08 00:00 [received]
PHST- 2018/05/08 00:00 [revised]
PHST- 2018/05/20 00:00 [accepted]
PHST- 2018/06/03 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/06/03 06:00 [entrez]
AID - S0923-1811(18)30233-0 [pii]
AID - 10.1016/j.jdermsci.2018.05.007 [doi]
PST - ppublish
SO  - J Dermatol Sci. 2018 Sep;91(3):268-275. doi: 10.1016/j.jdermsci.2018.05.007. Epub 
      2018 May 23.