PMID- 29858803
OWN - NLM
STAT- MEDLINE
DCOM- 20190304
LR  - 20190304
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1802
DP  - 2018
TI  - High-Throughput Contiguous Full-Length Next-Generation Sequencing of HLA Class I 
      and II Genes from 96 Donors in a Single MiSeq Run.
PG  - 89-100
LID - 10.1007/978-1-4939-8546-3_6 [doi]
AB  - The human leukocyte antigen (HLA) genes regulate and drive the immune system, and 
      are among the most polymorphic loci in the human genome. HLA diversity is known 
      to play an important role in transplantation and disease association studies. 
      There are multiple approaches to DNA-based HLA genotyping and recent advances in 
      next-generation sequencing (NGS) technologies have facilitated the development of 
      whole gene sequencing methods. We describe an accurate, efficient, scalable, and 
      cost-effective approach to contiguously amplify and sequence full-length genes of 
      six HLA class I and II loci from 96 individuals on a single Illumina MiSeq run.
FAU - Ehrenberg, Philip K
AU  - Ehrenberg PK
AD  - U.S. Military HIV Research Program (MHRP), Walter Reed Army Institute of 
      Research, Silver Spring, MD, USA.
FAU - Geretz, Aviva
AU  - Geretz A
AD  - U.S. Military HIV Research Program (MHRP), Walter Reed Army Institute of 
      Research, Silver Spring, MD, USA.
AD  - Henry M. Jackson Foundation for the Advancement of Military Medicine (HJF), 
      Bethesda, MD, USA.
FAU - Thomas, Rasmi
AU  - Thomas R
AD  - U.S. Military HIV Research Program (MHRP), Walter Reed Army Institute of 
      Research, Silver Spring, MD, USA. rthomas@hivresearch.org.
AD  - Henry M. Jackson Foundation for the Advancement of Military Medicine (HJF), 
      Bethesda, MD, USA. rthomas@hivresearch.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Gene Library
MH  - Genetic Loci
MH  - High-Throughput Nucleotide Sequencing/*methods
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Histocompatibility Antigens Class II/*genetics
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - *Tissue Donors
OTO - NOTNLM
OT  - Full-length amplification
OT  - HLA
OT  - HLA genotyping
OT  - Illumina
OT  - MIT-NGS
OT  - MiSeq
OT  - NGS
OT  - Next-generation sequencing
EDAT- 2018/06/03 06:00
MHDA- 2019/03/05 06:00
CRDT- 2018/06/03 06:00
PHST- 2018/06/03 06:00 [entrez]
PHST- 2018/06/03 06:00 [pubmed]
PHST- 2019/03/05 06:00 [medline]
AID - 10.1007/978-1-4939-8546-3_6 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1802:89-100. doi: 10.1007/978-1-4939-8546-3_6.