PMID- 29859892
OWN - NLM
STAT- MEDLINE
DCOM- 20181011
LR  - 20181011
IS  - 1769-7123 (Electronic)
IS  - 0923-2508 (Linking)
VI  - 169
IP  - 4-5
DP  - 2018 May-Jun
TI  - C-di-GMP turnover influences motility and biofilm formation in Bacillus 
      amyloliquefaciens PG12.
PG  - 205-213
LID - S0923-2508(18)30065-2 [pii]
LID - 10.1016/j.resmic.2018.04.009 [doi]
AB  - Bis-(3'-->5') cyclic dimeric guanosine monophosphate (c-di-GMP) is defined as a 
      highly versatile secondary messenger in bacteria, coordinating diverse aspects of 
      bacterial growth and behavior, including motility and biofilm formation. Bacillus 
      amyloliquefaciens PG12 is an effective biocontrol agent against apple ring rot 
      caused by Botryosphaeria dothidea. In this study, we characterized the core 
      regulators of c-di-GMP turnover in B. amyloliquefaciens PG12. Using bioinformatic 
      analysis, heterologous expression and biochemical characterization of knockout 
      and overexpression derivatives, we identified and characterized two active 
      diguanylate cyclases (which catalyze c-di-GMP biosynthesis), YhcK and YtrP and 
      one active c-di-GMP phosphodiesterase (which degrades c-di-GMP), YuxH. 
      Furthermore, we showed that elevating c-di-GMP levels up to a certain threshold 
      inhibited the swimming motility of B. amyloliquefaciens PG12. Although yhcK, ytrP 
      and yuxH knockout mutants did not display defects in biofilm formation, 
      significant increases in c-di-GMP levels induced by YtrP or YuxH overexpression 
      stimulated biofilm formation in B. amyloliquefaciens PG12. Our results indicate 
      that B. amyloliquefaciens possesses a functional c-di-GMP signaling system that 
      influences the bacterium's motility and ability to form biofilms. Since motility 
      and biofilm formation influence the efficacy of biological control agent, our 
      work provides a basis for engineering a more effective strain of B. 
      amyloliquefaciens PG12.
CI  - Copyright (c) 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights 
      reserved.
FAU - Yang, Yang
AU  - Yang Y
AD  - Department of Plant Pathology, College of Plant Protection, China Agricultural 
      University, Beijing 100193, China. Electronic address: yybiocontrol@163.com.
FAU - Li, Yan
AU  - Li Y
AD  - Department of Plant Pathology, College of Plant Protection, China Agricultural 
      University, Beijing 100193, China. Electronic address: liyancau@gmail.com.
FAU - Gao, Tantan
AU  - Gao T
AD  - Department of Plant Pathology, College of Plant Protection, China Agricultural 
      University, Beijing 100193, China. Electronic address: gaotantan0537@163.com.
FAU - Zhang, Yue
AU  - Zhang Y
AD  - Department of Plant Pathology, College of Plant Protection, China Agricultural 
      University, Beijing 100193, China. Electronic address: yzhangvae@163.com.
FAU - Wang, Qi
AU  - Wang Q
AD  - Department of Plant Pathology, College of Plant Protection, China Agricultural 
      University, Beijing 100193, China. Electronic address: wangqi@cau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180601
PL  - France
TA  - Res Microbiol
JT  - Research in microbiology
JID - 8907468
RN  - 0 (Biological Control Agents)
RN  - 0 (Escherichia coli Proteins)
RN  - 61093-23-0 (bis(3',5')-cyclic diguanylic acid)
RN  - EC 3.1.4.35 (3',5'-Cyclic-GMP Phosphodiesterases)
RN  - EC 4.6.- (Phosphorus-Oxygen Lyases)
RN  - EC 4.6.1.- (diguanylate cyclase)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - 3',5'-Cyclic-GMP Phosphodiesterases/*genetics/metabolism
MH  - Bacillus amyloliquefaciens/genetics/*metabolism
MH  - Biofilms/growth & development
MH  - Biological Control Agents
MH  - Cyclic GMP/*analogs & derivatives/metabolism
MH  - Escherichia coli Proteins/*genetics/metabolism
MH  - Gene Expression Regulation, Bacterial/*genetics
MH  - Gene Knockout Techniques
MH  - Movement
MH  - Phosphorus-Oxygen Lyases/*genetics/metabolism
MH  - Signal Transduction/genetics
OTO - NOTNLM
OT  - Bacillus amyloliquefaciens
OT  - Diguanylate cyclase
OT  - Gram-positive
OT  - Phosphodiesterase
EDAT- 2018/06/04 06:00
MHDA- 2018/10/12 06:00
CRDT- 2018/06/04 06:00
PHST- 2017/12/10 00:00 [received]
PHST- 2018/03/22 00:00 [revised]
PHST- 2018/04/05 00:00 [accepted]
PHST- 2018/06/04 06:00 [pubmed]
PHST- 2018/10/12 06:00 [medline]
PHST- 2018/06/04 06:00 [entrez]
AID - S0923-2508(18)30065-2 [pii]
AID - 10.1016/j.resmic.2018.04.009 [doi]
PST - ppublish
SO  - Res Microbiol. 2018 May-Jun;169(4-5):205-213. doi: 10.1016/j.resmic.2018.04.009. 
      Epub 2018 Jun 1.