PMID- 29860089
OWN - NLM
STAT- MEDLINE
DCOM- 20181012
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 116
DP  - 2018 Sep 30
TI  - Ultrasensitive dual probe immunosensor for the monitoring of nicotine 
      induced-brain derived neurotrophic factor released from cancer cells.
PG  - 108-115
LID - S0956-5663(18)30403-2 [pii]
LID - 10.1016/j.bios.2018.05.049 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) was detected in the extracellular matrix 
      of neuronal cells using a dual probe immunosensor (DPI), where one of them was 
      used as a working and another bioconjugate loading probe. The working probe was 
      fabricated by covalently immobilizing capture anti-BDNF (Cap Ab) on the gold 
      nanoparticles (AuNPs)/conducting polymer composite layer. The bioconjugate probe 
      was modified by drop casting a bioconjugate particles composed of conducting 
      polymer self-assembled AuNPs, immobilized with detection anti-BDNF (Det Ab) and 
      toluidine blue O (TBO). Each sensor layer was characterized using the surface 
      analysis and electrochemical methods. Two modified probes were precisely faced 
      each other to form a microfluidic channel structure and the gap between inside 
      modified surfaces was about 19 microm. At optimized conditions, the DPI showed a 
      linear dynamic range from 4.0 to 600.0 pg/ml with a detection limit of 
      1.5 +/- 0.012 pg/ml. Interference effect of IgG, arginine, glutamine, serine, 
      albumin, and fibrinogene were examined and stability of the developed biosensor 
      was also investigated. The reliability of the DPI sensor was evaluated by 
      monitoring the extracellular release of BDNF using exogenic activators (ethanol, 
      K(+), and nicotine) in neuronal and non-neuronal cells. In addition, the effect 
      of nicotine onto neuroblastoma cancer cells (SH-SY5Y) was studied in detail.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Akhtar, Mahmood H
AU  - Akhtar MH
AD  - Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), 
      Pusan National University, Busan 46241, South Korea.
FAU - Hussain, Khalil K
AU  - Hussain KK
AD  - Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), 
      Pusan National University, Busan 46241, South Korea.
FAU - Gurudatt, N G
AU  - Gurudatt NG
AD  - Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), 
      Pusan National University, Busan 46241, South Korea.
FAU - Chandra, Pranjal
AU  - Chandra P
AD  - Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), 
      Pusan National University, Busan 46241, South Korea; Department of Biosciences 
      and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, 
      Assam, India.
FAU - Shim, Yoon-Bo
AU  - Shim YB
AD  - Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), 
      Pusan National University, Busan 46241, South Korea. Electronic address: 
      ybshim@pusan.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20180528
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Polymers)
RN  - 6M3C89ZY6R (Nicotine)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Animals
MH  - Antibodies, Immobilized/chemistry/immunology
MH  - *Biosensing Techniques
MH  - Brain-Derived Neurotrophic Factor/*analysis/immunology/*metabolism
MH  - Cell Line, Tumor
MH  - Chlorocebus aethiops
MH  - Electrochemical Techniques
MH  - Gold/chemistry
MH  - Humans
MH  - *Immunoassay
MH  - Metal Nanoparticles/chemistry
MH  - Nanocomposites/chemistry
MH  - Neurons/drug effects
MH  - Nicotine/*pharmacology
MH  - Polymers/chemistry
MH  - Rats
MH  - Vero Cells
OTO - NOTNLM
OT  - Bioconjugate particles
OT  - Brain-derived neurotrophic factor
OT  - Dual probe sensor
OT  - Immunosensor
OT  - Neurotrophins
EDAT- 2018/06/04 06:00
MHDA- 2018/10/13 06:00
CRDT- 2018/06/04 06:00
PHST- 2018/04/13 00:00 [received]
PHST- 2018/05/22 00:00 [revised]
PHST- 2018/05/27 00:00 [accepted]
PHST- 2018/06/04 06:00 [pubmed]
PHST- 2018/10/13 06:00 [medline]
PHST- 2018/06/04 06:00 [entrez]
AID - S0956-5663(18)30403-2 [pii]
AID - 10.1016/j.bios.2018.05.049 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2018 Sep 30;116:108-115. doi: 10.1016/j.bios.2018.05.049. 
      Epub 2018 May 28.