PMID- 29860303
OWN - NLM
STAT- MEDLINE
DCOM- 20181106
LR  - 20240329
IS  - 1759-6653 (Electronic)
IS  - 1759-6653 (Linking)
VI  - 10
IP  - 7
DP  - 2018 Jul 1
TI  - The Targeted Sequencing of Alpha Satellite DNA in Cercopithecus pogonias Provides 
      New Insight Into the Diversity and Dynamics of Centromeric Repeats in Old World 
      Monkeys.
PG  - 1837-1851
LID - 10.1093/gbe/evy109 [doi]
AB  - Alpha satellite is the major repeated DNA element of primate centromeres. 
      Specific evolutionary mechanisms have led to a great diversity of sequence 
      families with peculiar genomic organization and distribution, which have till now 
      been studied mostly in great apes. Using high throughput sequencing of alpha 
      satellite monomers obtained by enzymatic digestion followed by computational and 
      cytogenetic analysis, we compare here the diversity and genomic distribution of 
      alpha satellite DNA in two related Old World monkey species, Cercopithecus 
      pogonias and Cercopithecus solatus, which are known to have diverged about 7 Ma. 
      Two main families of monomers, called C1 and C2, are found in both species. A 
      detailed analysis of our data sets revealed the existence of numerous subfamilies 
      within the centromeric C1 family. Although the most abundant subfamily is 
      conserved between both species, our fluorescence in situ hybridization (FISH) 
      experiments clearly show that some subfamilies are specific for each species and 
      that their distribution is restricted to a subset of chromosomes, thereby 
      pointing to the existence of recurrent amplification/homogenization events. The 
      pericentromeric C2 family is very abundant on the short arm of all acrocentric 
      chromosomes in both species, pointing to specific mechanisms that lead to this 
      distribution. Results obtained using two different restriction enzymes are fully 
      consistent with a predominant monomeric organization of alpha satellite DNA that 
      coexists with higher order organization patterns in the C. pogonias genome. Our 
      study suggests a high dynamics of alpha satellite DNA in Cercopithecini, with 
      recurrent apparition of new sequence variants and interchromosomal sequence 
      transfer.
FAU - Cacheux, Lauriane
AU  - Cacheux L
AD  - Departement Adaptations du Vivant, Structure et Instabilite des Genomes, INSERM 
      U1154, CNRS UMR7196, Sorbonne Universites, Museum National d'Histoire Naturelle, 
      Paris, France.
AD  - Departement Origines et Evolution, Institut de Systematique, Evolution, 
      Biodiversite, UMR 7205 MNHN, CNRS, UPMC, EPHE, Sorbonne Universites, Museum 
      National d'Histoire Naturelle, Paris, France.
FAU - Ponger, Loic
AU  - Ponger L
AD  - Departement Adaptations du Vivant, Structure et Instabilite des Genomes, INSERM 
      U1154, CNRS UMR7196, Sorbonne Universites, Museum National d'Histoire Naturelle, 
      Paris, France.
FAU - Gerbault-Seureau, Michele
AU  - Gerbault-Seureau M
AD  - Departement Origines et Evolution, Institut de Systematique, Evolution, 
      Biodiversite, UMR 7205 MNHN, CNRS, UPMC, EPHE, Sorbonne Universites, Museum 
      National d'Histoire Naturelle, Paris, France.
FAU - Loll, Francois
AU  - Loll F
AD  - Departement Adaptations du Vivant, Structure et Instabilite des Genomes, INSERM 
      U1154, CNRS UMR7196, Sorbonne Universites, Museum National d'Histoire Naturelle, 
      Paris, France.
FAU - Gey, Delphine
AU  - Gey D
AD  - Service de Systematique Moleculaire, UMS 2700 CNRS, Sorbonne Universites, Museum 
      National d'Histoire Naturelle, Paris, France.
FAU - Richard, Florence Anne
AU  - Richard FA
AD  - Departement Origines et Evolution, Institut de Systematique, Evolution, 
      Biodiversite, UMR 7205 MNHN, CNRS, UPMC, EPHE, Sorbonne Universites, Museum 
      National d'Histoire Naturelle, Paris, France.
AD  - Universite Versailles St-Quentin, Montigny-le-Bretonneux, France.
FAU - Escude, Christophe
AU  - Escude C
AD  - Departement Adaptations du Vivant, Structure et Instabilite des Genomes, INSERM 
      U1154, CNRS UMR7196, Sorbonne Universites, Museum National d'Histoire Naturelle, 
      Paris, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Genome Biol Evol
JT  - Genome biology and evolution
JID - 101509707
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Centromere/*genetics
MH  - Cercopithecidae/genetics
MH  - Cercopithecus/*genetics
MH  - Consensus Sequence
MH  - DNA, Satellite/*genetics
MH  - Evolution, Molecular
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Male
MH  - Minisatellite Repeats
MH  - Sequence Analysis, DNA
PMC - PMC6061836
EDAT- 2018/06/04 06:00
MHDA- 2018/11/07 06:00
PMCR- 2018/06/01
CRDT- 2018/06/04 06:00
PHST- 2018/05/29 00:00 [accepted]
PHST- 2018/06/04 06:00 [pubmed]
PHST- 2018/11/07 06:00 [medline]
PHST- 2018/06/04 06:00 [entrez]
PHST- 2018/06/01 00:00 [pmc-release]
AID - 5026596 [pii]
AID - evy109 [pii]
AID - 10.1093/gbe/evy109 [doi]
PST - ppublish
SO  - Genome Biol Evol. 2018 Jul 1;10(7):1837-1851. doi: 10.1093/gbe/evy109.