PMID- 29860902
OWN - NLM
STAT- MEDLINE
DCOM- 20190918
LR  - 20190918
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 27
IP  - 6
DP  - 2018 Jun
TI  - Effects of Brain-Derived Neurotrophic Factor on MicroRNA Expression Profile in 
      Human Endothelial Progenitor Cells.
PG  - 1005-1009
LID - 10.1177/0963689718761658 [doi]
AB  - The mechanisms underlying proangiogenic function of brain-derived neurotrophic 
      factor (BDNF) are not fully understood. The current study was designed to explore 
      the microRNA (miRNA) profile in human early endothelial progenitor cells (EPCs, 
      also referred to as CFU-Hill cells) treated with BDNF. Treatment of early EPCs 
      with BDNF for 7 d significantly increased the colony formation of outgrowth 
      endothelial cells. BDNF suppressed the expression of miR-4716-5p, miR-3928, 
      miR-433, miR-1294, miR-1539, and miR-19b-1*. In contrast, BDNF significantly 
      increased the levels of miR-432*, miR-4499, miR-3911, miR-1183, miR-4669, 
      miR-636, miR-4717-3p, miR-4298, miR485-5p, and miR-181c. Since miR-433 has been 
      reported to augment hematopoietic cells proliferation and differentiation, we 
      examined the role of miR-433 in regenerative effects of BDNF. BDNF stimulated the 
      protein expression of guanylate-binding protein 2 via the suppression of miR-433. 
      However, the knockdown of miR-433 was not sufficient to significantly increase 
      the number of outgrowth endothelial cell colonies, suggesting that modulation of 
      miR-433 alone does not stimulate regenerative capacity of EPCs. In aggregate, our 
      results also suggest that the effect of BDNF on regenerative function of EPCs may 
      depend on complex changes in the expression of microRNAs.
FAU - He, Tongrong
AU  - He T
AD  - 1 Department of Anesthesiology and Molecular Pharmacology and Experimental 
      Therapeutics, Mayo Clinic College of Medicine, Rochester, MN, USA.
FAU - Sun, Ruohan
AU  - Sun R
AD  - 1 Department of Anesthesiology and Molecular Pharmacology and Experimental 
      Therapeutics, Mayo Clinic College of Medicine, Rochester, MN, USA.
AD  - 2 Department of Neurology, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang Province, China.
FAU - Li, Ying
AU  - Li Y
AD  - 3 Department of Biomedical Statistics and Informatics, Mayo Clinic College of 
      Medicine, Rochester, MN, USA.
FAU - Katusic, Zvonimir S
AU  - Katusic ZS
AD  - 1 Department of Anesthesiology and Molecular Pharmacology and Experimental 
      Therapeutics, Mayo Clinic College of Medicine, Rochester, MN, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180604
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cells, Cultured
MH  - Endothelial Progenitor Cells/*metabolism
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - *Transcriptome
PMC - PMC6050915
OTO - NOTNLM
OT  - angiogenesis
OT  - brain-derived neurotrophic factor
OT  - endothelial progenitor cells
OT  - miR-433
OT  - microRNA
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2018/06/05 06:00
MHDA- 2019/09/19 06:00
PMCR- 2018/06/01
CRDT- 2018/06/05 06:00
PHST- 2018/06/05 06:00 [pubmed]
PHST- 2019/09/19 06:00 [medline]
PHST- 2018/06/05 06:00 [entrez]
PHST- 2018/06/01 00:00 [pmc-release]
AID - 10.1177_0963689718761658 [pii]
AID - 10.1177/0963689718761658 [doi]
PST - ppublish
SO  - Cell Transplant. 2018 Jun;27(6):1005-1009. doi: 10.1177/0963689718761658. Epub 
      2018 Jun 4.