PMID- 29861828
OWN - NLM
STAT- MEDLINE
DCOM- 20181002
LR  - 20181114
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2018
DP  - 2018
TI  - Protective Role of Antioxidant Huskless Barley Extracts on TNF-alpha-Induced 
      Endothelial Dysfunction in Human Vascular Endothelial Cells.
PG  - 3846029
LID - 10.1155/2018/3846029 [doi]
LID - 3846029
AB  - Oxidative stress and inflammation are considered as two key factors that 
      contribute to the development of atherosclerosis. This study was to investigate 
      the antioxidant capacity of huskless barley and to explore its protective 
      functions through the regulation of the antioxidant defense and inflammatory 
      response in human umbilical vein endothelial cells (HUVEC). The oxygen radical 
      absorbance capacity (ORAC), ferric-reducing antioxidant power (FRAP), and 
      2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) 
      scavenging capacity of water and alkali extracts of the polysaccharides from nine 
      huskless barley varieties were investigated in vitro. The antioxidant properties 
      of the alkaline extracts were more pronounced than those of the water extracts. 
      The results from the cell model showed that pretreatment of HUVEC with the water 
      or alkaline extracts of the polysaccharides from the huskless barley cultivars 
      QHH and NLGL decreased the levels of reactive oxygen species (ROS), monocyte 
      chemotactic protein 1 (MCP-1), and vascular cell adhesion molecule 1 (VCAM-1) but 
      increased the level of superoxide dismutase (SOD) and maintained cell viability. 
      Huskless barley polysaccharide extracts exhibited the vasodilatory effect of 
      inhibiting angiotensin-converting enzyme (ACE) production. These discoveries 
      revealed the potent protective functions of barley in oxidative damage and a 
      potential role for barley in preventing chronic inflammation in cardiovascular 
      diseases.
FAU - Liao, Zhanghua
AU  - Liao Z
AD  - Department of Food Science and Engineering, School of Agriculture and Biology, 
      Shanghai Jiao Tong University, Shanghai 200240, China.
FAU - Cai, Haoran
AU  - Cai H
AD  - Department of Food Science and Engineering, School of Agriculture and Biology, 
      Shanghai Jiao Tong University, Shanghai 200240, China.
FAU - Xu, Zekun
AU  - Xu Z
AD  - Department of Food Science and Engineering, School of Agriculture and Biology, 
      Shanghai Jiao Tong University, Shanghai 200240, China.
FAU - Wang, Jing
AU  - Wang J
AD  - College of Chemical Engineering, Nanjing Forestry University, No. 159 Longpan 
      Road, Nanjing 210037, China.
FAU - Qiu, Chen
AU  - Qiu C
AD  - Department of Food Science and Engineering, School of Agriculture and Biology, 
      Shanghai Jiao Tong University, Shanghai 200240, China.
FAU - Xie, Jing
AU  - Xie J
AD  - Department of Food Science and Engineering, School of Agriculture and Biology, 
      Shanghai Jiao Tong University, Shanghai 200240, China.
FAU - Huang, Wuyang
AU  - Huang W
AUID- ORCID: 0000-0002-3038-0636
AD  - Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, 
      Nanjing 210014, China.
AD  - Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Jiangsu 
      Academy of Agricultural Sciences, Nanjing 210014, China.
FAU - Sui, Zhongquan
AU  - Sui Z
AUID- ORCID: 0000-0002-5316-117X
AD  - Department of Food Science and Engineering, School of Agriculture and Biology, 
      Shanghai Jiao Tong University, Shanghai 200240, China.
LA  - eng
PT  - Journal Article
DEP - 20180510
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Antioxidants)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.4.15.1 (Peptidyl-Dipeptidase A)
SB  - IM
MH  - Antioxidants/chemistry/isolation & purification/*pharmacology
MH  - Cell Survival/drug effects
MH  - Hordeum/*chemistry/metabolism
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Oxidative Stress/*drug effects
MH  - Peptidyl-Dipeptidase A/metabolism
MH  - Plant Extracts/*chemistry/metabolism
MH  - Polysaccharides/chemistry/isolation & purification/pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Vascular Cell Adhesion Molecule-1/metabolism
PMC - PMC5971280
EDAT- 2018/06/05 06:00
MHDA- 2018/10/03 06:00
PMCR- 2018/05/10
CRDT- 2018/06/05 06:00
PHST- 2017/12/14 00:00 [received]
PHST- 2018/03/17 00:00 [revised]
PHST- 2018/03/27 00:00 [accepted]
PHST- 2018/06/05 06:00 [entrez]
PHST- 2018/06/05 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/05/10 00:00 [pmc-release]
AID - 10.1155/2018/3846029 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2018 May 10;2018:3846029. doi: 10.1155/2018/3846029. 
      eCollection 2018.