PMID- 29863270
OWN - NLM
STAT- MEDLINE
DCOM- 20191030
LR  - 20191030
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 22
IP  - 10
DP  - 2018 May
TI  - Electrophysiological effects of melatonin on rat trigeminal ganglion neurons that 
      participate in nociception in vitro.
PG  - 3234-3239
LID - 15086 [pii]
LID - 10.26355/eurrev_201805_15086 [doi]
AB  - OBJECTIVE: Melatonin (MT) is a hormone mainly produced by the pineal gland. It 
      may be involved in the regulation of nociception, the mechanisms of which remain 
      unclear. In the present study, electrophysiological effects of MT on neurons were 
      studied. MATERIALS AND METHODS: The cultured neurons were isolated from 
      Sprague-Dawley rats trigeminal ganglia (TG). The neuron was voltage clamped using 
      the whole cell patch clamp technique. We recorded resting membrane potential, 
      action potential threshold and number, action potential duration and 
      GABA-activated inward currents in the presence of 0.01 muM, 10 muM MT, and in the 
      absence of MT. RESULTS: In the presence of high concentration of MT, the 
      spontaneous action potential disappeared and action potential threshold was 
      significantly increased. GABA-activated inward currents were recorded and blocked 
      by GABAA receptor antagonist, bicuculline, in the majority of TG neurons (91% 
      40/44). Continuous perfusion of MT could cause a decrease of GABA-activated 
      currents. The inhibiting effect was dose-dependent and irreversible. CONCLUSIONS: 
      The results suggest that MT has several electrophysiological effects on TG 
      neurons, which may be involved in the peripheral mechanisms of orofacial pain.
FAU - Huang, F
AU  - Huang F
AD  - Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China. 
      chengbin@mail.sysu.edu.cn.
FAU - Guan, X-Y
AU  - Guan XY
FAU - Yan, Y
AU  - Yan Y
FAU - Fan, W-G
AU  - Fan WG
FAU - You, Y-Y
AU  - You YY
FAU - He, H-W
AU  - He HW
FAU - Cheng, B
AU  - Cheng B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - JL5DK93RCL (Melatonin)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - Action Potentials/drug effects/*physiology
MH  - Animals
MH  - Bicuculline/pharmacology
MH  - Cells, Cultured
MH  - Male
MH  - Melatonin/*metabolism/*pharmacology
MH  - Membrane Potentials/drug effects/physiology
MH  - Neurons/drug effects/physiology
MH  - Nociception/drug effects/*physiology
MH  - Rats
MH  - Trigeminal Ganglion/drug effects/*physiology
EDAT- 2018/06/05 06:00
MHDA- 2019/10/31 06:00
CRDT- 2018/06/05 06:00
PHST- 2018/06/05 06:00 [entrez]
PHST- 2018/06/05 06:00 [pubmed]
PHST- 2019/10/31 06:00 [medline]
AID - 15086 [pii]
AID - 10.26355/eurrev_201805_15086 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2018 May;22(10):3234-3239. doi: 
      10.26355/eurrev_201805_15086.