PMID- 29864618 OWN - NLM STAT- MEDLINE DCOM- 20190122 LR - 20190122 IS - 1872-7123 (Electronic) IS - 0165-1781 (Linking) VI - 266 DP - 2018 Aug TI - Late gestation immune activation increases IBA1-positive immunoreactivity levels in the corpus callosum of adult rat offspring. PG - 175-185 LID - S0165-1781(17)32101-7 [pii] LID - 10.1016/j.psychres.2018.05.063 [doi] AB - Animal models of maternal immune activation study the effects of infection, an environmental risk factor for schizophrenia, on brain development. Microglia activation and cytokine upregulation may have key roles in schizophrenia neuropathology. We hypothesised that maternal immune activation induces changes in microglia and cytokines in the brains of the adult offspring. Maternal immune activation was induced by injecting polyriboinosinic:polyribocytidylic acid into pregnant rats on gestational day (GD) 10 or GD19, with brain tissue collected from the offspring at adulthood. We observed no change in Iba1, Gfap, IL1-beta and TNF-alpha mRNA levels in the cingulate cortex (CC) in adult offspring exposed to maternal immune activation. Prenatal exposure to immune activation had a significant main effect on microglial IBA1-positive immunoreactive material (IBA1+IRM) in the corpus callosum; post-hoc analyses identified a significant increase in GD19 offspring, but not GD10. No change in was observed in the CC. In contrast, maternal immune activation had a significant main effect on GFAP+IRM in the CC at GD19 (not GD10); post-hoc analyses only identified a strong trend towards increased GFAP+IRM in the GD19 offspring, with no white matter changes. This suggests late gestation maternal immune activation causes subtle alterations to microglia and astrocytes in the adult offspring. CI - Crown Copyright (c) 2018. Published by Elsevier B.V. All rights reserved. FAU - Duchatel, Ryan J AU - Duchatel RJ AD - School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW 2308, Australia; Priority Centre for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Ryan.Duchatel@uon.edu.au. FAU - Meehan, Crystal L AU - Meehan CL AD - School of Psychology, Faculty of Science, University of Newcastle, Callaghan, NSW 2308, Australia; Priority Centre for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Crystal.Meehan@utas.edu.au. FAU - Harms, Lauren R AU - Harms LR AD - School of Psychology, Faculty of Science, University of Newcastle, Callaghan, NSW 2308, Australia; Priority Centre for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Lauren.Harms@newcastle.edu.au. FAU - Michie, Patricia T AU - Michie PT AD - School of Psychology, Faculty of Science, University of Newcastle, Callaghan, NSW 2308, Australia; Priority Centre for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Pat.Michie@newcastle.edu.au. FAU - Bigland, Mark J AU - Bigland MJ AD - School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW 2308, Australia; Priority Centre for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Mark.Bigland@uon.edu.au. FAU - Smith, Doug W AU - Smith DW AD - School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW 2308, Australia; Priority Centre for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Douglas.Smith@newcastle.edu.au. FAU - Walker, Frederick R AU - Walker FR AD - School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Rohan.Walker@newcastle.edu.au. FAU - Jobling, Phillip AU - Jobling P AD - School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW 2308, Australia; Priority Centre for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Phillip.Jobling@newcastle.edu.au. FAU - Hodgson, Deborah M AU - Hodgson DM AD - School of Psychology, Faculty of Science, University of Newcastle, Callaghan, NSW 2308, Australia; Priority Centre for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Deborah.Hodgson@newcastle.edu.au. FAU - Tooney, Paul A AU - Tooney PA AD - School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW 2308, Australia; Priority Centre for Brain and Mental Health Research, University of Newcastle, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, New Lambton Heights, NSW 2305, Australia. Electronic address: Paul.Tooney@newcastle.edu.au. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180525 PL - Ireland TA - Psychiatry Res JT - Psychiatry research JID - 7911385 RN - 0 (Aif1 protein, rat) RN - 0 (Biomarkers) RN - 0 (Calcium-Binding Proteins) RN - 0 (Microfilament Proteins) RN - O84C90HH2L (Poly I-C) SB - IM MH - Age Factors MH - Animals MH - Biomarkers/metabolism MH - Calcium-Binding Proteins/*immunology/metabolism MH - Corpus Callosum/drug effects/*immunology/metabolism MH - Female MH - Immunity, Cellular/drug effects/*immunology MH - Male MH - Microfilament Proteins/*immunology/metabolism MH - Microglia/drug effects/immunology/metabolism MH - Poly I-C/pharmacology MH - Pregnancy MH - Prenatal Exposure Delayed Effects/chemically induced/*immunology/metabolism MH - Rats MH - Rats, Wistar MH - Schizophrenia/immunology/metabolism OTO - NOTNLM OT - Astrocytes OT - Brain OT - Cingulate cortex OT - Immunohistochemistry OT - Microglia OT - Prenatal infection OT - Real-time PCR OT - Schizophrenia OT - White matter EDAT- 2018/06/05 06:00 MHDA- 2019/01/23 06:00 CRDT- 2018/06/05 06:00 PHST- 2017/11/16 00:00 [received] PHST- 2018/03/20 00:00 [revised] PHST- 2018/05/23 00:00 [accepted] PHST- 2018/06/05 06:00 [pubmed] PHST- 2019/01/23 06:00 [medline] PHST- 2018/06/05 06:00 [entrez] AID - S0165-1781(17)32101-7 [pii] AID - 10.1016/j.psychres.2018.05.063 [doi] PST - ppublish SO - Psychiatry Res. 2018 Aug;266:175-185. doi: 10.1016/j.psychres.2018.05.063. Epub 2018 May 25.