PMID- 29864621
OWN - NLM
STAT- MEDLINE
DCOM- 20181106
LR  - 20211204
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 105
DP  - 2018 Sep
TI  - Downregulation of circular RNA hsa_circ_0001649 indicates poor prognosis for 
      retinoblastoma and regulates cell proliferation and apoptosis via AKT/mTOR 
      signaling pathway.
PG  - 326-333
LID - S0753-3322(18)32716-1 [pii]
LID - 10.1016/j.biopha.2018.05.141 [doi]
AB  - Retinoblastoma (RB) is the most common intraocular malignancy in infants and 
      children with high mortality rate in developing countries. Emerging evidence 
      demonstrated that abnormally expressed circular RNAs (circRNAs) are involved in 
      tumorigenesis and progression in several malignancies. However, their clinical 
      values, biological functions and mechanisms in RB has not been reported before. 
      Recently, hsa_circ_0001649 was found to play imperative roles in 
      cholangiocarcinoma, gastric cancer, and hepatocellular carcinoma. In the current 
      study, qRT-PCR was performed to detect the expression of hsa_circ_0001649 in RB 
      samples and cells. The correlations between hsa_circ_0001649 expression and 
      clinicopathologic characteristics were further analyzed. In addition, we 
      up-regulated hsa_circ_0001649 in Y79 cells and knocked down hsa_circ_0001649 in 
      WERI-Rb1 cells to explore its effect on cell proliferation and apoptosis. The 
      animal study was performed to confirm the in vitro results. Furthermore, AKT/mTOR 
      signaling pathway was detected to clarify the molecular mechanisms of 
      hsa_circ_0001649 exerts in RB cell growth. The results indicated that 
      hsa_circ_0001649 was decreased in RB tissues and cells, and this downregulation 
      was associated with larger tumor size and advanced intraocular international 
      retinoblastoma classify (IIRC) stage in RB patients. Additionally, 
      hsa_circ_0001649 could act as an independent prognostic predictor for overall 
      survival in patients with RB. Moreover, hsa_circ_0001649 inhibits cell growth and 
      promotes cell apoptosis in RB cells. AKT/mTOR signaling pathway is involved in 
      the cell growth alteration affected by hsa_circ_0001649. Overall, 
      hsa_circ_0001649 might be a potentially useful prognostic biomarker and 
      therapeutic target for RB.
CI  - Copyright (c) 2018. Published by Elsevier Masson SAS.
FAU - Xing, Lichen
AU  - Xing L
AD  - Department of Ophthalmology, Third Affiliated Hospital of Qiqihar Medical 
      University, Heilongjiang Province, 161000, China.
FAU - Zhang, Leiming
AU  - Zhang L
AD  - Department of Ophthalmology, Third Affiliated Hospital of Qiqihar Medical 
      University, Heilongjiang Province, 161000, China.
FAU - Feng, Yali
AU  - Feng Y
AD  - Department of Ophthalmology, Third Affiliated Hospital of Qiqihar Medical 
      University, Heilongjiang Province, 161000, China.
FAU - Cui, Zhe
AU  - Cui Z
AD  - Department of Ophthalmology, Third Affiliated Hospital of Qiqihar Medical 
      University, Heilongjiang Province, 161000, China.
FAU - Ding, Lin
AU  - Ding L
AD  - Department of Clinical Skills Experimental Teaching Center, Qiqihar Medical 
      University, Heilongjiang Province, 161000, China. Electronic address: 
      dlineye@126.com.
LA  - eng
PT  - Journal Article
DEP - 20180601
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (RNA, Circular)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Child, Preschool
MH  - Down-Regulation/*genetics
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Infant
MH  - Male
MH  - Mice, Nude
MH  - Multivariate Analysis
MH  - Neoplasm Invasiveness
MH  - Phenotype
MH  - Prognosis
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - RNA/*genetics
MH  - RNA, Circular
MH  - Retinoblastoma/*genetics/*pathology/surgery
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - AKT/mTOR pathway
OT  - Circular RNA
OT  - Prognosis
OT  - Retinoblastoma
OT  - hsa_circ_0001649
EDAT- 2018/06/05 06:00
MHDA- 2018/11/07 06:00
CRDT- 2018/06/05 06:00
PHST- 2018/04/23 00:00 [received]
PHST- 2018/05/26 00:00 [revised]
PHST- 2018/05/28 00:00 [accepted]
PHST- 2018/06/05 06:00 [pubmed]
PHST- 2018/11/07 06:00 [medline]
PHST- 2018/06/05 06:00 [entrez]
AID - S0753-3322(18)32716-1 [pii]
AID - 10.1016/j.biopha.2018.05.141 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2018 Sep;105:326-333. doi: 10.1016/j.biopha.2018.05.141. 
      Epub 2018 Jun 1.