PMID- 29864961
OWN - NLM
STAT- MEDLINE
DCOM- 20181011
LR  - 20181011
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 103
DP  - 2018 Jul
TI  - Catalpol inhibits migration and induces apoptosis in gastric cancer cells and in 
      athymic nude mice.
PG  - 1708-1719
LID - S0753-3322(17)37013-0 [pii]
LID - 10.1016/j.biopha.2018.03.094 [doi]
AB  - Gastric cancer is one of the leading factors, causing tumor-associated death 
      worldwide. However, due to the limited therapeutic strategies in inhibition of 
      gastric cancer, further studies are still required to develop effective 
      treatments. In the present study, we attempted to explore the effects of 
      catalpol, extracted from a traditional Chinese herb Rehmannia glutinosa, on 
      gastric cancer progression in cells and in xenograft nude mice. The results 
      indicated that catalpol dose-dependently reduced the proliferation of cancer 
      cells. The migrated cells were also decreased with catalpol treatment, as 
      evidenced by the down-regulated expressions of matrix metalloproteinase-2 
      (MMP-2), alpha-smooth muscle actin (alpha-SMA), Ras homolog gene family, member A 
      (RhoA), Rho kinase 1 (ROCK1) and N-cadherin. Further, catalpol induced apoptosis 
      in gastric cancer cells. Apoptosis-related markers including cleaved Caspase-3 
      and PARP were highly expressed in catalpol-treated cells. However, the cells with 
      pre-treatment of caspases inhibitor reversed catalpol-induced apoptosis. Further, 
      catalpol also enhanced reactive oxygen species (ROS) generation in gastric cancer 
      cells, which was eliminated by N-acetylcystein (NAC) pre-incubation, an important 
      ROS scavenger. Of note, catalpol potentiated the anti-cancer effects of cisplatin 
      (DDP) in suppressing gastric cancer cells. In vivo, catalpol prevented the tumor 
      growth in xenograft nude mice, while no significant difference was observed in 
      body weight. The immunohistochemical analysis showed that catalpol increased the 
      number of terminal deoxynucleotidyl transferase dUTP nick end labelling 
      (TUNEL)-positive cells, whereas decreased the number of KI-67-positive cells. 
      Together, the results above indicated that catalpol has a potential value in 
      treating gastric cancer.
CI  - Copyright (c) 2018. Published by Elsevier Masson SAS.
FAU - Wang, Zheng-Hua
AU  - Wang ZH
AD  - The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 
      121001, China.
FAU - Zhan-Sheng, Hu
AU  - Zhan-Sheng H
AD  - The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 
      121001, China. Electronic address: duyaowuexome@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20180514
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Iridoid Glucosides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 2415-24-9 (catalpol)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology/therapeutic use
MH  - Apoptosis/*drug effects
MH  - Cell Cycle Checkpoints/drug effects
MH  - Cell Line, Tumor
MH  - Cell Movement/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Cisplatin/pharmacology/therapeutic use
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Iridoid Glucosides/chemistry/*pharmacology/therapeutic use
MH  - Male
MH  - Mice, Nude
MH  - RNA, Messenger/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Stomach Neoplasms/genetics/*pathology
OTO - NOTNLM
OT  - Apoptosis and ROS
OT  - Catalpol
OT  - DDP
OT  - Gastric cancer
OT  - Migration
EDAT- 2018/06/06 06:00
MHDA- 2018/10/12 06:00
CRDT- 2018/06/06 06:00
PHST- 2017/12/20 00:00 [received]
PHST- 2018/03/15 00:00 [revised]
PHST- 2018/03/15 00:00 [accepted]
PHST- 2018/06/06 06:00 [entrez]
PHST- 2018/06/06 06:00 [pubmed]
PHST- 2018/10/12 06:00 [medline]
AID - S0753-3322(17)37013-0 [pii]
AID - 10.1016/j.biopha.2018.03.094 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2018 Jul;103:1708-1719. doi: 10.1016/j.biopha.2018.03.094. 
      Epub 2018 May 14.