PMID- 29868468
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220317
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 8
DP  - 2018
TI  - Association of Mouse Mammary Tumor Virus With Human Breast Cancer: Histology, 
      Immunohistochemistry and Polymerase Chain Reaction Analyses.
PG  - 141
LID - 10.3389/fonc.2018.00141 [doi]
LID - 141
AB  - PURPOSE: The purpose of this study is to determine whether mouse mammary tumor 
      virus (MMTV)-associated human breast cancer has the same or similar histology to 
      MMTV-associated mouse mammary tumors. Such associations may indicate a role for 
      MMTV in human breast cancer. METHODS: Immunohistochemical techniques (using 
      antibodies directed against the signal peptide p14 of the envelope precursor 
      protein of MMTV) and polymerase chain reaction (PCR) analyses were used to 
      identify MMTV proteins and MMTV-like envelope gene sequences in a series of 
      breast cancers from Australian women. The histological characteristics of these 
      human breast cancer specimens were compared with MMTV positive mouse mammary 
      tumors. The same methods were used to study benign breast tissues which 
      1-11 years later developed into breast cancer. RESULTS: MMTV p14 proteins were 
      identified in 27 (54%) of 50 human breast cancers. MMTV env gene sequences were 
      identified by PCR in 12 (27%) of 45 human breast cancers. There was a significant 
      correlation between the presence of MMTV (identified by p14 immunohistochemistry) 
      in human breast cancers and histological characteristics similar to MMTV positive 
      mouse mammary tumors (p = 0.001). There was a non-significant correlation between 
      the presence of MMTV env gene sequences (identified by PCR) in human breast 
      cancers and histological characteristics similar to MMTV positive mouse mammary 
      tumors (p = 0.290). MMTV p14 proteins were identified in 7 (54%) of 13 benign 
      breast specimens that later developed into human breast cancers. MMTV by PCR was 
      identified in two benign specimens one of whom later developed MMTV positive 
      breast cancer. DISCUSSION: These observations offer evidence that MMTV may be 
      associated with characteristic human breast cancer histology. p14-based 
      immunohistochemistry appears to be a more reliable technique than PCR for the 
      identification of MMTV in human breast cancer. Identification of MMTV-associated 
      p14 proteins in benign breast tissues confirms prior PCR-based studies that MMTV 
      infection occurs before the development of MMTV positive breast cancer. 
      CONCLUSION: Many MMTV positive human breast cancers have similar histology to 
      MMTV positive mouse mammary tumors. MMTV infection identified in benign breast 
      tissues precedes development of MMTV positive human breast cancer. When 
      considered in the context of prior studies, these observations indicate a likely 
      role for MMTV in human breast cancer.
FAU - Lawson, James S
AU  - Lawson JS
AD  - University of New South Wales, Sydney, NSW, Australia.
FAU - Mazzanti, Chiara
AU  - Mazzanti C
AD  - Fondazione Pisana per la Scienza Onlus, Pisa, Italy.
FAU - Civita, Prospero
AU  - Civita P
AD  - Fondazione Pisana per la Scienza Onlus, Pisa, Italy.
FAU - Menicagli, Michele
AU  - Menicagli M
AD  - Fondazione Pisana per la Scienza Onlus, Pisa, Italy.
FAU - Ngan, Christopher C
AU  - Ngan CC
AD  - University of New South Wales, Sydney, NSW, Australia.
FAU - Whitaker, Noel J
AU  - Whitaker NJ
AD  - University of New South Wales, Sydney, NSW, Australia.
FAU - Hochman, Jacob
AU  - Hochman J
AD  - Department of Cell and Developmental Biology, Alexander Silberman Institute of 
      Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
FAU - Braitbard, Ori
AU  - Braitbard O
AD  - Department of Cell and Developmental Biology, Alexander Silberman Institute of 
      Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
FAU - Yosufi, Benafsha
AU  - Yosufi B
AD  - Douglass Hanly Moir Pathology, Macquarie Park, NSW, Australia.
FAU - Glenn, Wendy K
AU  - Glenn WK
AD  - University of New South Wales, Sydney, NSW, Australia.
LA  - eng
PT  - Journal Article
DEP - 20180507
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC5950654
OTO - NOTNLM
OT  - breast cancer
OT  - histology
OT  - immunohistochemistry
OT  - mouse mammary tumor virus
OT  - mouse mammary tumor virus p14
OT  - mouse mammary tumor virus signal peptide
OT  - mouse mammary tumors
OT  - polymerase chain reaction
EDAT- 2018/06/06 06:00
MHDA- 2018/06/06 06:01
PMCR- 2018/01/01
CRDT- 2018/06/06 06:00
PHST- 2018/01/30 00:00 [received]
PHST- 2018/04/18 00:00 [accepted]
PHST- 2018/06/06 06:00 [entrez]
PHST- 2018/06/06 06:00 [pubmed]
PHST- 2018/06/06 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2018.00141 [doi]
PST - epublish
SO  - Front Oncol. 2018 May 7;8:141. doi: 10.3389/fonc.2018.00141. eCollection 2018.