PMID- 29872311
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220317
IS  - 1178-6930 (Print)
IS  - 1178-6930 (Electronic)
IS  - 1178-6930 (Linking)
VI  - 11
DP  - 2018
TI  - The methylation profiles of PRDM promoters in non-small cell lung cancer.
PG  - 2991-3002
LID - 10.2147/OTT.S156775 [doi]
AB  - BACKGROUND: Non-small cell lung cancer (NSCLC) is one of the leading malignant 
      tumors worldwide. Aberrant gene promoter methylation contributes to NSCLC, and 
      PRDM is a tumor suppressor gene family that possesses histone methyltransferase 
      activity. This study aimed to investigate whether aberrant methylation of PRDM 
      promoter is involved in NSCLC. MATERIALS AND METHODS: Primary tumor tissues, 
      adjacent nontumorous tissues, and distant lung tissues were collected from 75 
      NSCLC patients including 52 lung squamous cell carcinoma (LSCC) patients and 23 
      lung adenocarcinoma patients. The expression of PRDMs was detected by polymerase 
      chain reaction (PCR), Western blot, and immunohistochemical analysis. The 
      methylation of PRDM promoters was detected by methylation-specific PCR. The 
      correlation of methylation and expression of PRDMs with clinicopathological 
      characteristics of patients were analyzed. RESULTS: mRNA expression of PRDM2, 
      PRDM5, and PRDM16 was low or absent in tumor tissues compared to distant lung 
      tissues. The methylation frequencies of PRDM2, PRDM5, and PRDM16 in tumor tissues 
      were significantly higher than those in distal lung tissues. In LSCC patients, 
      methylation of PRDM2 and PRDM16 was correlated with smoking status and 
      methylation of PRDM5 was correlated with tumor differentiation. CONCLUSION: The 
      expression of PRDM2, PRDM5, and PRDM16 is low or absent in NSCLC, and this is 
      mainly due to gene promoter methylation. Smoking may be an important cause of 
      PRDM2 and PRDM16 methylation in NSCLC.
FAU - Tan, Shuang-Xiang
AU  - Tan SX
AD  - Hunan Province Institute of Gerontology, Hunan Provincial People's Hospital/The 
      First Affiliated Hospital of Hunan Normal University, Changsha, China.
AD  - Department of Respiratory Medicine, Hunan Provincial People's Hospital/The First 
      Affiliated Hospital of Hunan Normal University, Changsha, China.
FAU - Hu, Rui-Cheng
AU  - Hu RC
AD  - Hunan Province Institute of Gerontology, Hunan Provincial People's Hospital/The 
      First Affiliated Hospital of Hunan Normal University, Changsha, China.
AD  - Department of Respiratory Medicine, Hunan Provincial People's Hospital/The First 
      Affiliated Hospital of Hunan Normal University, Changsha, China.
FAU - Xia, Qian
AU  - Xia Q
AD  - Department of Respiratory Medicine, Hunan Provincial People's Hospital/The First 
      Affiliated Hospital of Hunan Normal University, Changsha, China.
FAU - Tan, Yong-Li
AU  - Tan YL
AD  - Hunan Province Institute of Gerontology, Hunan Provincial People's Hospital/The 
      First Affiliated Hospital of Hunan Normal University, Changsha, China.
FAU - Liu, Jing-Jing
AU  - Liu JJ
AD  - Hunan Province Institute of Gerontology, Hunan Provincial People's Hospital/The 
      First Affiliated Hospital of Hunan Normal University, Changsha, China.
FAU - Gan, Gui-Xiang
AU  - Gan GX
AD  - Hunan Province Institute of Gerontology, Hunan Provincial People's Hospital/The 
      First Affiliated Hospital of Hunan Normal University, Changsha, China.
FAU - Wang, Li-le
AU  - Wang LL
AD  - Hunan Province Institute of Gerontology, Hunan Provincial People's Hospital/The 
      First Affiliated Hospital of Hunan Normal University, Changsha, China.
LA  - eng
PT  - Journal Article
DEP - 20180522
PL  - New Zealand
TA  - Onco Targets Ther
JT  - OncoTargets and therapy
JID - 101514322
PMC - PMC5973400
OTO - NOTNLM
OT  - PR domain zinc finger protein
OT  - epigenetics
OT  - gene expression
OT  - lung tumor
OT  - methylation
COIS- Disclosure The authors report no conflicts of interest in this work.
EDAT- 2018/06/07 06:00
MHDA- 2018/06/07 06:01
PMCR- 2018/05/22
CRDT- 2018/06/07 06:00
PHST- 2018/06/07 06:00 [entrez]
PHST- 2018/06/07 06:00 [pubmed]
PHST- 2018/06/07 06:01 [medline]
PHST- 2018/05/22 00:00 [pmc-release]
AID - ott-11-2991 [pii]
AID - 10.2147/OTT.S156775 [doi]
PST - epublish
SO  - Onco Targets Ther. 2018 May 22;11:2991-3002. doi: 10.2147/OTT.S156775. 
      eCollection 2018.