PMID- 29872440
OWN - NLM
STAT- MEDLINE
DCOM- 20190730
LR  - 20231112
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 9
DP  - 2018
TI  - Tannic Acid-Modified Silver and Gold Nanoparticles as Novel Stimulators of 
      Dendritic Cells Activation.
PG  - 1115
LID - 10.3389/fimmu.2018.01115 [doi]
LID - 1115
AB  - Silver nanoparticles (AgNPs) are promising new antimicrobial agents against a 
      wide range of skin and mucosal pathogens. However, their interaction with the 
      immune system is currently not fully understood. Dendritic cells (DCs) are 
      crucial during development of T cell-specific responses against bacterial and 
      viral pathogens. We have previously shown that tannic acid-modified silver 
      nanoparticles (TA-AgNPs) consist of a promising microbicide against HSV-2. The 
      aim of this study was to compare the ability of TA-AgNPs or TA-AuNPs of similar 
      sizes (TA-Ag/AuNPs) to induce DCs maturation and activation in the presence of 
      HSV-2 antigens when used at non-toxic doses. First, we used JAWS II DC line to 
      test toxicity, ultrastructure as well as activation markers (MHC I and II, CD40, 
      CD80, CD86, PD-L1) and cytokine production in the presence of TA-Ag/AuNPs. 
      Preparations of HSV-2 treated with nanoparticles (TA-Ag/AuNPs-HSV-2) were further 
      used to investigate HSV-2 antigen uptake, activation markers, TLR9 expression, 
      and cytokine production. Additionally, we accessed proliferation and activation 
      of HSV-2-specific T cells by DCs treated with TA-AgNP/AuNPs-HSV-2. We found that 
      both TA-AgNPs and TA-AuNPs were efficiently internalized by DCs and induced 
      activated ultrastructure. Although TA-AgNPs were more toxic than TA-AuNPs in 
      corresponding sizes, they were also more potent stimulators of DCs maturation and 
      TLR9 expression. TA-Ag/AuNPs-HSV-2 helped to overcome inhibition of DCs 
      maturation by live or inactivated virus through up-regulation of MHC II and CD86 
      and down-regulation of CD80 expression. Down-regulation of CD40 expression in 
      HSV-2-infected DCs was reversed when HSV-2 was treated with TA-NPs sized >30 nm. 
      On the other hand, small-sized TA-AgNPs helped to better internalize HSV-2 
      antigens. HSV-2 treated with both types of NPs stimulated activation of JAWS II 
      and memory CD8+ T cells, while TA-AgNPs treatment induced IFN-gamma producing 
      CD4+ and CD8+ T cells. Our study shows that TA-AgNPs or TA-AuNPs are good 
      activators of DCs, albeit their final effect upon maturation and activation may 
      be metal and size dependent. We conclude that TA-Ag/AuNPs consist of a novel 
      class of nano-adjuvants, which can help to overcome virus-induced suppression of 
      DCs activation.
FAU - Orlowski, Piotr
AU  - Orlowski P
AD  - Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
FAU - Tomaszewska, Emilia
AU  - Tomaszewska E
AD  - Department of Materials Technology and Chemistry, Faculty of Chemistry, 
      University of Lodz, Lodz, Poland.
FAU - Ranoszek-Soliwoda, Katarzyna
AU  - Ranoszek-Soliwoda K
AD  - Department of Materials Technology and Chemistry, Faculty of Chemistry, 
      University of Lodz, Lodz, Poland.
FAU - Gniadek, Marianna
AU  - Gniadek M
AD  - Faculty of Chemistry, University of Warsaw, Warsaw, Poland.
FAU - Labedz, Olga
AU  - Labedz O
AD  - Faculty of Chemistry, University of Warsaw, Warsaw, Poland.
FAU - Malewski, Tadeusz
AU  - Malewski T
AD  - Museum and Institute of Zoology, Polish Academy of Science, Warsaw, Poland.
FAU - Nowakowska, Julita
AU  - Nowakowska J
AD  - Laboratory of Electron and Confocal Microscopy, Faculty of Biology, University of 
      Warsaw, Warsaw, Poland.
FAU - Chodaczek, Grzegorz
AU  - Chodaczek G
AD  - Wroclaw Research Centrum EIT+, Wroclaw, Poland.
FAU - Celichowski, Grzegorz
AU  - Celichowski G
AD  - Department of Materials Technology and Chemistry, Faculty of Chemistry, 
      University of Lodz, Lodz, Poland.
FAU - Grobelny, Jaroslaw
AU  - Grobelny J
AD  - Department of Materials Technology and Chemistry, Faculty of Chemistry, 
      University of Lodz, Lodz, Poland.
FAU - Krzyzowska, Malgorzata
AU  - Krzyzowska M
AD  - Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
AD  - Wroclaw Research Centrum EIT+, Wroclaw, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180522
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Tannins)
RN  - 3M4G523W1G (Silver)
RN  - 7440-57-5 (Gold)
MH  - Animals
MH  - Biomarkers
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/metabolism/ultrastructure
MH  - Gene Expression
MH  - *Gold/chemistry
MH  - Herpesvirus 2, Human/immunology
MH  - Immunophenotyping
MH  - Lymphocyte Activation/genetics/immunology
MH  - *Metal Nanoparticles/chemistry/ultrastructure
MH  - Mice
MH  - *Silver/chemistry
MH  - T-Lymphocytes/immunology/metabolism
MH  - *Tannins/chemistry
PMC - PMC5972285
OTO - NOTNLM
OT  - HSV-2
OT  - dendritic cells
OT  - gold nanoparticles
OT  - silver nanoparticles
OT  - tannic acid
EDAT- 2018/06/07 06:00
MHDA- 2018/06/07 06:01
PMCR- 2018/01/01
CRDT- 2018/06/07 06:00
PHST- 2018/02/11 00:00 [received]
PHST- 2018/05/03 00:00 [accepted]
PHST- 2018/06/07 06:00 [entrez]
PHST- 2018/06/07 06:00 [pubmed]
PHST- 2018/06/07 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2018.01115 [doi]
PST - epublish
SO  - Front Immunol. 2018 May 22;9:1115. doi: 10.3389/fimmu.2018.01115. eCollection 
      2018.