PMID- 29872532
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240318
IS  - 2662-6810 (Print)
IS  - 2052-7276 (Electronic)
IS  - 2052-7276 (Linking)
VI  - 5
DP  - 2018
TI  - Tomato SlAN11 regulates flavonoid biosynthesis and seed dormancy by interaction 
      with bHLH proteins but not with MYB proteins.
PG  - 27
LID - 10.1038/s41438-018-0032-3 [doi]
LID - 27
AB  - The flavonoid compounds are important secondary metabolites with versatile human 
      nutritive benefits and fulfill a multitude of functions during plant growth and 
      development. The abundance of different flavonoid compounds are finely tuned with 
      species-specific pattern by a ternary MBW complex, which consists of a MYB, a 
      bHLH, and a WD40 protein, but the essential role of SlAN11, which is a WD40 
      protein, is not fully understood in tomato until now. In this study, a tomato 
      WD40 protein named as SlAN11 was characterized as an effective transcription 
      regulator to promote plant anthocyanin and seed proanthocyanidin (PA) contents, 
      with late flavonoid biosynthetic genes activated in 35S::SlAN11 transgenic lines, 
      while the dihydroflavonol flow to the accumulation of flavonols or their 
      glycosylated derivatives was reduced by repressing the expression of SlFLS in 
      this SlAN11-overexpressed lines. The above changes were reversed in 
      35S::SlAN11-RNAi transgenic lines except remained levels of flavonol compounds 
      and SlFLS expression. Interestingly, our data revealed that SlAN11 gene could 
      affect seed dormancy by regulating the expressions of abscisic acid (ABA) 
      signaling-related genes SlABI3 and SlABI5, and the sensitivity to ABA treatment 
      in seed germination is conversely changed by SlAN11-overexpressed or 
      -downregulated lines. Yeast two-hybrid assays demonstrated that SlAN11 interacted 
      with bHLH but not with MYB proteins in the ternary MBW complex, whereas bHLH 
      interacted with MYB in tomato. Our results indicated that low level of 
      anthocyanins in tomato fruits, with low expression of bHLH (SlTT8) and MYB 
      (SlANT1 and SlAN2) genes, remain unchanged upon modification of SlAN11 gene alone 
      in the transgenic lines. These results suggest that the tomato WD40 protein 
      SlAN11, coordinating with bHLH and MYB proteins, plays a crucial role in the fine 
      adjustment of the flavonoid biosynthesis and seed dormancy in tomato.
FAU - Gao, Yongfeng
AU  - Gao Y
AD  - 1School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010 China. ISNI: 0000 0004 1808 3334. GRID: 
      grid.440649.b
FAU - Liu, Jikai
AU  - Liu J
AD  - 1School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010 China. ISNI: 0000 0004 1808 3334. GRID: 
      grid.440649.b
FAU - Chen, Yongfu
AU  - Chen Y
AD  - 1School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010 China. ISNI: 0000 0004 1808 3334. GRID: 
      grid.440649.b
FAU - Tang, Hai
AU  - Tang H
AD  - 1School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010 China. ISNI: 0000 0004 1808 3334. GRID: 
      grid.440649.b
FAU - Wang, Yang
AU  - Wang Y
AD  - 1School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010 China. ISNI: 0000 0004 1808 3334. GRID: 
      grid.440649.b
FAU - He, Yongmei
AU  - He Y
AD  - Yunnan Engineering Laboratory for Agro-environment Pollution Control and 
      Eco-remediation, The Innovation Team for Farmland Non-pollution Production of 
      Yunnan Province, Kunming, 650201 China.
FAU - Ou, Yongbin
AU  - Ou Y
AD  - 1School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010 China. ISNI: 0000 0004 1808 3334. GRID: 
      grid.440649.b
FAU - Sun, Xiaochun
AU  - Sun X
AD  - 3Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources 
      Industrialization, Shaanxi University of Chinese Medicine, Xianyang, 712046 
      China. ISNI: 0000 0004 0646 966X. GRID: grid.449637.b
FAU - Wang, Songhu
AU  - Wang S
AD  - 4Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041 
      China. ISNI: 0000000119573309. GRID: grid.9227.e
FAU - Yao, Yinan
AU  - Yao Y
AD  - 1School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010 China. ISNI: 0000 0004 1808 3334. GRID: 
      grid.440649.b
LA  - eng
PT  - Journal Article
DEP - 20180601
PL  - England
TA  - Hortic Res
JT  - Horticulture research
JID - 101655540
PMC - PMC5981465
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/06/07 06:00
MHDA- 2018/06/07 06:01
PMCR- 2018/06/01
CRDT- 2018/06/07 06:00
PHST- 2017/11/28 00:00 [received]
PHST- 2018/03/03 00:00 [revised]
PHST- 2018/03/11 00:00 [accepted]
PHST- 2018/06/07 06:00 [entrez]
PHST- 2018/06/07 06:00 [pubmed]
PHST- 2018/06/07 06:01 [medline]
PHST- 2018/06/01 00:00 [pmc-release]
AID - 32 [pii]
AID - 10.1038/s41438-018-0032-3 [doi]
PST - epublish
SO  - Hortic Res. 2018 Jun 1;5:27. doi: 10.1038/s41438-018-0032-3. eCollection 2018.