PMID- 29872772
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240318
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 4
IP  - 6
DP  - 2018 Jun
TI  - The effects of repeated antibiotic administration to juvenile BALB/c mice on the 
      microbiota status and animal behavior at the adult age.
PG  - e00644
LID - 10.1016/j.heliyon.2018.e00644 [doi]
LID - e00644
AB  - Recent studies carried on germ -free (GF) animal models suggest that the gut 
      microbiota (GM) may play a role in the regulation of anxiety, mood, and cognitive 
      abilities such as memory and learning processes. Consistently, any treatment 
      disturbing the gut microbiota, including the overuse of antibiotics, may 
      influence the brain functions and impact behavior. In the present study, to 
      address this issue, two wide-spectrum antibiotics (ampicillin and cefoperazone, 1 
      g/l) were repeatedly applied throughout a 6-week period to initially 21-day-old 
      male BALB/c mice. Antibiotics were administered separately or in a mixed fashion. 
      On the completion of the antibiotic treatment, all mice were subjected to the 
      behavioral tests. The serum levels of corticosterone and brain-derived 
      neurotropic factor (BDNF) were assessed. Gut microbiota profiles were obtained by 
      using denaturing gradient gel electrophoresis system, DGGE, from fecal samples. 
      Ampicillin had a greater impact on both, gut microbiota composition and mice 
      behavior compared to cefoperazone. All antibiotic-treated groups manifested a 
      decrease in the locomotor activity and reduced recognition memory. However, the 
      ampicillin-treated groups showed a higher anxiety level as assessed by the open 
      field and the elevated plus maze tests and an increased immobility (behavioral 
      despair) in the forced swim test. Obtained results evidently show that in mice, a 
      repeated antibiotic treatment applied during adolescence, parallel to the changes 
      in GM, affects locomotor activity, affective behavior and cognitive skills in 
      young adults with ampicillin specifically enhancing anxiety- and depressive-like 
      responses. Lower levels of serum BDNF were not associated with cognitive 
      impairment but with changes in affective-like behaviors. Repeated administration 
      of neither ampicillin nor cefoperazone affected basal serum corticosterone 
      levels. This is one of the few studies demonstrating changes in a behavioral 
      phenotype of young-adult subjects who were previously exposed to a repeated 
      antibiotic treatment.
FAU - Ceylani, Taha
AU  - Ceylani T
AD  - Department of Biological Sciences, Middle East Technical University, 06800, 
      Ankara, Turkey.
AD  - Department of Molecular Biology and Genetics, Mus Alparslan University, 49250, 
      Mus, Turkey.
FAU - Jakubowska-Dogru, Ewa
AU  - Jakubowska-Dogru E
AD  - Department of Biological Sciences, Middle East Technical University, 06800, 
      Ankara, Turkey.
FAU - Gurbanov, Rafig
AU  - Gurbanov R
AD  - Department of Molecular Biology and Genetics, Bilecik S.E. University, 11230, 
      Bilecik, Turkey.
FAU - Teker, Hikmet Taner
AU  - Teker HT
AD  - Department of Biological Sciences, Middle East Technical University, 06800, 
      Ankara, Turkey.
FAU - Gozen, Ayse Gul
AU  - Gozen AG
AD  - Department of Biological Sciences, Middle East Technical University, 06800, 
      Ankara, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20180604
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC5986162
OTO - NOTNLM
OT  - Microbiology
OT  - Neuroscience
EDAT- 2018/06/07 06:00
MHDA- 2018/06/07 06:01
PMCR- 2018/06/04
CRDT- 2018/06/07 06:00
PHST- 2018/03/16 00:00 [received]
PHST- 2018/04/02 00:00 [revised]
PHST- 2018/05/30 00:00 [accepted]
PHST- 2018/06/07 06:00 [entrez]
PHST- 2018/06/07 06:00 [pubmed]
PHST- 2018/06/07 06:01 [medline]
PHST- 2018/06/04 00:00 [pmc-release]
AID - S2405-8440(18)30806-5 [pii]
AID - e00644 [pii]
AID - 10.1016/j.heliyon.2018.e00644 [doi]
PST - epublish
SO  - Heliyon. 2018 Jun 4;4(6):e00644. doi: 10.1016/j.heliyon.2018.e00644. eCollection 
      2018 Jun.