PMID- 29872807
OWN - NLM
STAT- MEDLINE
DCOM- 20190123
LR  - 20190123
IS  - 2040-3372 (Electronic)
IS  - 2040-3364 (Linking)
VI  - 10
IP  - 23
DP  - 2018 Jun 14
TI  - DNA-templated silver nanoclusters locate microRNAs in the nuclei of gastric 
      cancer cells.
PG  - 11079-11090
LID - 10.1039/c8nr02634c [doi]
AB  - Dysregulation of microRNAs (miRNAs) is correlated with cancer progression. In 
      vitro detection methods using extracts from cell lysis cannot provide information 
      about the spatial distribution of miRNAs. Due to the development of miRNA 
      fluorescence in situ hybridization (FISH), increasing amounts of intracellular 
      expression information are being obtained. However, miRNA FISH suffers from weak 
      signals and complex steps and thus remains very challenging. Herein, a strategy 
      based on DNA-templated silver nanoclusters (AgNCs/DNAs) and their G-rich 
      fluorescence enhancement effect was developed for FISH detection of miRNAs in 
      gastric cancer cells. The method combines hybridization and signal amplification 
      into one step, which allows imaging of intracellular miRNAs immediately after 
      hybridization. Most importantly, using the method based on our design, 
      miR-101-3p, miR-16-5p and miR-19b-3p were found to be located in the nuclei of 
      MGC803 cells with granulated shapes, indicating an unanticipated distribution 
      pattern. In addition, before the final miRNA FISH, we performed an optimization 
      of AgNCs/DNAs and their G-rich fluorescence enhancement effect; we found that the 
      effect occurred at shorter wavelengths emitting green fluorescence, with weakened 
      red fluorescence at longer wavelengths. However, the components involved in the 
      FISH process impacted the fluorescence properties so greatly that the probes 
      finally exhibited slightly strengthened red fluorescence signals. Our method 
      enables facile visualization of miRNAs at the subcellular level, which may 
      benefit the precise localization of miRNAs in single cells in the future.
FAU - Zhang, Jingpu
AU  - Zhang J
AD  - Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and 
      Microfabrication of the Ministry of Education, Department of Instrument Science 
      and Engineering, School of Electronic Information and Electrical Engineering, 
      Shanghai Jiao Tong University, P. R. China. dxcui@sjtu.edu.cn.
FAU - Liu, Yanlei
AU  - Liu Y
FAU - Zhi, Xiao
AU  - Zhi X
FAU - Zhang, Chunlei
AU  - Zhang C
FAU - Liu, Tie Fu
AU  - Liu TF
FAU - Cui, Daxiang
AU  - Cui D
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nanoscale
JT  - Nanoscale
JID - 101525249
RN  - 0 (DNA Probes)
RN  - 0 (MicroRNAs)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Cell Line, Tumor
MH  - DNA Probes/*chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Metal Nanoparticles
MH  - MicroRNAs/*analysis
MH  - *Silver
MH  - Stomach Neoplasms/*genetics
EDAT- 2018/06/07 06:00
MHDA- 2019/01/24 06:00
CRDT- 2018/06/07 06:00
PHST- 2018/06/07 06:00 [pubmed]
PHST- 2019/01/24 06:00 [medline]
PHST- 2018/06/07 06:00 [entrez]
AID - 10.1039/c8nr02634c [doi]
PST - ppublish
SO  - Nanoscale. 2018 Jun 14;10(23):11079-11090. doi: 10.1039/c8nr02634c.