PMID- 29874406
OWN - NLM
STAT- MEDLINE
DCOM- 20191030
LR  - 20191030
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Print)
IS  - 0022-3751 (Linking)
VI  - 596
IP  - 16
DP  - 2018 Aug
TI  - The anatomical pathway from the mesodiencephalic junction to the inferior olive 
      relays perioral sensory signals to the cerebellum in the mouse.
PG  - 3775-3791
LID - 10.1113/JP275836 [doi]
AB  - KEY POINTS: Perioral tactile signals are transmitted via the infraorbital nerve 
      (ION) to trigeminal nuclei. Each cerebellar Purkinje cell (PC) receives this 
      signal as complex spikes (CSs) via a climbing fibre (CF) emerging from the 
      inferior olive (IO). The anatomical pathway from trigeminal nuclei to the IO is 
      not clearly identified. In the present study, we examined candidate anatomical 
      pathways for perioral sensory signalling by analysing CSs recorded from PCs in 
      male mice by single unit recording. CS generation by ION stimulation was 
      inhibited by injection of a GABA(A) receptor agonist, muscimol, into the 
      contralateral mesodiencephalic junction, which is referred to as the area 
      parafascicularis prerubralis (PfPr). The number of CSs evoked by mechanical 
      whisker stimulation was also decreased by contralateral PfPr inhibition. These 
      results suggest the existence of a sensory signalling pathway to the IO via the 
      PfPr in mice. ABSTRACT: Perioral tactile signals are transmitted via the 
      infraorbital nerve (ION) to trigeminal nuclei. Each cerebellar Purkinje cell 
      receives this signal as complex spikes (CSs) via a climbing fibre emerging from 
      the inferior olive (IO). However, the anatomical pathway from the trigeminal 
      nuclei to the IO is not clearly identified. In the present study, we recorded CSs 
      from Purkinje cells in male mice by single unit recording, and examined the 
      signal transduction pathway. CSs were evoked by electrical stimulation of the 
      ipsilateral or contralateral ION with a latency of 20-70 ms. CS generation by 
      ipsilateral ION stimulation was inhibited by injection of a GABA(A) receptor 
      agonist, muscimol, into the contralateral mesodiencephalic junction, ranging from 
      around the fasciculus retroflexus to the interstitial nucleus of Cajal, which is 
      referred to as the area parafascicularis prerubralis (PfPr). CSs evoked by 
      contralateral ION stimulation were also suppressed by muscimol injection into the 
      PfPr, although the effective area was more restricted. Furthermore, CSs evoked by 
      mechanical stimulation around the whisker region were suppressed by PfPr 
      inhibition. We also found that the primary motor cortex plays a role to suppress 
      this signalling pathway. These results indicate the existence of an anatomical 
      pathway for conducting perioral sensory signals to the IO via the PfPr.
CI  - (c) 2018 The Authors The Journal of Physiology published by John Wiley & Sons Ltd 
      on behalf of The Physiological Society.
FAU - Kubo, Reika
AU  - Kubo R
AD  - Department of Neurophysiology, Graduate School of Biomedical and Health Sciences, 
      Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
FAU - Aiba, Atsu
AU  - Aiba A
AD  - Laboratory of Animal Resources, Center for Disease Biology and Integrative 
      Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, 
      Japan.
FAU - Hashimoto, Kouichi
AU  - Hashimoto K
AUID- ORCID: 0000-0002-3884-8213
AD  - Department of Neurophysiology, Graduate School of Biomedical and Health Sciences, 
      Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180624
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (GABA-A Receptor Agonists)
RN  - 0 (Receptors, GABA-A)
RN  - 2763-96-4 (Muscimol)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cerebellum/cytology/drug effects/*physiology
MH  - Diencephalon/cytology/drug effects/*physiology
MH  - GABA-A Receptor Agonists/pharmacology
MH  - Male
MH  - Mesencephalon/cytology/drug effects/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mouth/cytology/drug effects/*physiology
MH  - Muscimol/pharmacology
MH  - Olivary Nucleus/cytology/drug effects/*physiology
MH  - Purkinje Cells/cytology/drug effects/*physiology
MH  - Receptors, GABA-A/chemistry
MH  - Sensory Receptor Cells/cytology/drug effects/*physiology
PMC - PMC6092293
OTO - NOTNLM
OT  - Inferior olive
OT  - Sensory transduction
OT  - cerebellum
OT  - complex spike
OT  - mesodiencephalic junction
EDAT- 2018/06/07 06:00
MHDA- 2019/10/31 06:00
PMCR- 2018/06/24
CRDT- 2018/06/07 06:00
PHST- 2018/01/13 00:00 [received]
PHST- 2018/05/14 00:00 [accepted]
PHST- 2018/06/07 06:00 [pubmed]
PHST- 2019/10/31 06:00 [medline]
PHST- 2018/06/07 06:00 [entrez]
PHST- 2018/06/24 00:00 [pmc-release]
AID - TJP13052 [pii]
AID - 10.1113/JP275836 [doi]
PST - ppublish
SO  - J Physiol. 2018 Aug;596(16):3775-3791. doi: 10.1113/JP275836. Epub 2018 Jun 24.