PMID- 29875586
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220331
IS  - 1016-3190 (Print)
IS  - 2223-8956 (Electronic)
VI  - 30
IP  - 2
DP  - 2018 Apr-Jun
TI  - Human umbilical cord-derived mesenchymal stem cells reduce monosodium 
      iodoacetate-induced apoptosis in cartilage.
PG  - 71-80
LID - 10.4103/tcmj.tcmj_23_18 [doi]
AB  - OBJECTIVE: The present study investigated the therapeutic potential and 
      underlying mechanisms of human umbilical cord mesenchymal stem cells (HUCMSCs) on 
      joint cartilage destruction induced by monosodium iodoacetate (MIA) in mice. 
      MATERIALS AND METHODS: HUCMSCs were tested for mesenchymal stem cell (MSC) 
      characteristics including surface markers by flow cytometry and mesoderm 
      differentiation (adipogenesis, osteogenesis, and chondrogenesis). Terminal 
      deoxynucleotidyl transferase dUTP nick end labeling assay and Western blot assay 
      were used to evaluate MIA-induced chondrocyte apoptosis. In the in vivo study, 18 
      mice were divided into three groups (n = 6 each); normal saline (control), 
      MIA-treated, and MIA-treated/HUCMSC-transplantation. Rota-Rods tests were used to 
      evaluate MIA-induced cartilage destruction behaviors in mice. Histological 
      changes in the mice cartilage were examined by immunohistochemistry. RESULTS: 
      HUCMSCs had an immunophenotype similar to bone marrow-derived MSCs and were able 
      to differentiate into adipocytes, osteocytes, and chondrocytes. Conditioned 
      medium of the HUCMSCs exhibited an anti-apoptotic effect and inhibited expression 
      of caspase 3 in MIA-treated chondrocytes. HUCMSC transplantation assisted in 
      recovery from movement impairment (from 30% on day 7 to 115% on day 14) and in 
      regeneration and repair of cartilage damaged by MIA. (International Cartilage 
      Repair Society score: 3.8 in the MIA group vs. 10.2 in the HUCMSC-treated group); 
      HUCMSC transplantation ameliorated cartilage apoptosis through the caspase 3 
      pathway in MIA-induced cartilage destruction in mice. CONCLUSION: Taken together, 
      these observations suggest that HUCMSC transplantation appears to be effective in 
      protecting cartilage from MIA damage.
FAU - Chang, Yu-Hsun
AU  - Chang YH
AD  - Department of Pediatrics, Buddhist Tzu Chi General Hospital, Hualien, Taiwan.
AD  - Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
FAU - Wu, Kun-Chi
AU  - Wu KC
AD  - Department of Orthopedics, Buddhist Tzu Chi General Hospital and Tzu Chi 
      University, Hualien, Taiwan.
FAU - Liu, Hwan-Wun
AU  - Liu HW
AD  - Department of Occupational Medicine, Buddhist Tzu Chi General Hospital and Tzu 
      Chi University, Hualien, Taiwan.
FAU - Chu, Tang-Yuan
AU  - Chu TY
AD  - Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
AD  - Department of Obstetrics and Gynecology, Buddhist Tzu Chi General Hospital and 
      Tzu Chi University, Hualien, Taiwan.
FAU - Ding, Dah-Ching
AU  - Ding DC
AD  - Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
AD  - Department of Obstetrics and Gynecology, Buddhist Tzu Chi General Hospital and 
      Tzu Chi University, Hualien, Taiwan.
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Ci Ji Yi Xue Za Zhi
JT  - Ci ji yi xue za zhi = Tzu-chi medical journal
JID - 9514171
PMC - PMC5968746
OTO - NOTNLM
OT  - Apoptosis
OT  - Capase3
OT  - Human umbilical cord stromal cells
OT  - Monosodium iodoacetate
OT  - Osteoarthritis
COIS- There are no conflicts of interest.
EDAT- 2018/06/08 06:00
MHDA- 2018/06/08 06:01
PMCR- 2018/04/01
CRDT- 2018/06/08 06:00
PHST- 2018/06/08 06:00 [entrez]
PHST- 2018/06/08 06:00 [pubmed]
PHST- 2018/06/08 06:01 [medline]
PHST- 2018/04/01 00:00 [pmc-release]
AID - TCMJ-30-71 [pii]
AID - 10.4103/tcmj.tcmj_23_18 [doi]
PST - ppublish
SO  - Ci Ji Yi Xue Za Zhi. 2018 Apr-Jun;30(2):71-80. doi: 10.4103/tcmj.tcmj_23_18.