PMID- 2988091
OWN - NLM
STAT- MEDLINE
DCOM- 19850709
LR  - 20061115
IS  - 0034-5288 (Print)
IS  - 0034-5288 (Linking)
VI  - 38
IP  - 2
DP  - 1985 Mar
TI  - Detection of antibodies against infectious bursal disease virus: a comparison of 
      three serological methods.
PG  - 189-92
AB  - Four different oil emulsion infectious bursal disease virus (IBDV) vaccines were 
      inoculated into four-week-old specific pathogen-free chickens. At weekly 
      intervals for five weeks, sera were obtained from the vaccinated birds and from 
      uninoculated control birds and examined for antibodies against IBDV by 
      enzyme-linked immunosorbent assay (ELISA), the quantitative agar gel precipitin 
      (QAGP) test and the virus neutralisation (VN) test. There was a highly 
      significant correlation between the mean responses to all tests; the highest 
      correlation (0.818) was between VN and QAGP and the lowest (0.573) between QAGP 
      and ELISA. Generally the ELISA detected positive sera earlier than the VN test 
      which in turn was more sensitive than the QAGP test. The ELISA and QAGP test were 
      less variable, more reproducible and easier to perform than the VN test.
FAU - Nicholas, R A
AU  - Nicholas RA
FAU - Reed, N E
AU  - Reed NE
FAU - Wood, G W
AU  - Wood GW
FAU - Hebert, C N
AU  - Hebert CN
FAU - Muskett, J C
AU  - Muskett JC
FAU - Thornton, D H
AU  - Thornton DH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*analysis
MH  - Chickens/*immunology
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - Immunodiffusion/*veterinary
MH  - *Immunoenzyme Techniques
MH  - Infectious bursal disease virus/*immunology
MH  - Injections, Intramuscular/veterinary
MH  - Injections, Subcutaneous/veterinary
MH  - *Neutralization Tests
MH  - Reoviridae/*immunology
MH  - Viral Vaccines/administration & dosage/*immunology
EDAT- 1985/03/01 00:00
MHDA- 1985/03/01 00:01
CRDT- 1985/03/01 00:00
PHST- 1985/03/01 00:00 [pubmed]
PHST- 1985/03/01 00:01 [medline]
PHST- 1985/03/01 00:00 [entrez]
PST - ppublish
SO  - Res Vet Sci. 1985 Mar;38(2):189-92.