PMID- 29882916
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20181114
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 19
IP  - 6
DP  - 2018 May 24
TI  - Supplementation with IL-6 and Muscle Cell Culture Conditioned Media Enhances 
      Myogenic Differentiation of Adipose Tissue-Derived Stem Cells through STAT3 
      Activation.
LID - 10.3390/ijms19061557 [doi]
LID - 1557
AB  - Mature skeletal muscle cells cannot be expanded in culture systems. Therefore, it 
      is difficult to construct an in vitro model for muscle diseases. To establish an 
      efficient protocol for myogenic differentiation of human adipose tissue-derived 
      stem cells (hADSCs), we investigated whether addition of IL-6 and/or 
      myocyte-conditioned media (CM) to conventional differentiation media can shorten 
      the differentiation period. hADSCs were differentiated to myocytes using the 
      conventional protocol or modified with the addition of 25 pg/mL IL-6 and/or C2C12 
      CM (25% v/v). The expression of MyoD and myogenine mRNA was significantly higher 
      at 5(-)6 days after differentiation using the modified protocol than with the 
      conventional protocol. mRNA and protein expression of myosin heavy chain, a 
      marker of myotubes, was significantly upregulated at 28 and 42 days of 
      differentiation using the modified protocol, and the level achieved after a 
      4-week differentiation period was similar to that achieved at 6 weeks using the 
      conventional protocol. The expression of p-STAT3 was significantly increased when 
      the modified protocol was used. Similarly, addition of colivelin, a STAT3 
      activator, instead of IL-6 and C2C12 CM, promoted the myogenic differentiation of 
      ADSCs. The modified protocol improved differentiation efficiency and reduced the 
      time required for differentiation of myocytes. It might be helpful to save cost 
      and time when preparing myocytes for cell therapies and drug discovery.
FAU - Seo, Eunhui
AU  - Seo E
AD  - College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon 
      University, Incheon 21936, Korea. eunhuiseo@gachon.ac.kr.
AD  - Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, 
      Korea. eunhuiseo@gachon.ac.kr.
FAU - Kang, Hwansu
AU  - Kang H
AD  - College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon 
      University, Incheon 21936, Korea. hwansu83@naver.com.
FAU - Lim, Oh-Kyung
AU  - Lim OK
AD  - Department of Rehabilitation Medicine, Gil Hospital, Incheon 21565, Korea. 
      phmed@gilhospital.com.
FAU - Jun, Hee-Sook
AU  - Jun HS
AUID- ORCID: 0000-0002-1166-4932
AD  - College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon 
      University, Incheon 21936, Korea. hsjun@gachon.ac.kr.
AD  - Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, 
      Korea. hsjun@gachon.ac.kr.
AD  - Gachon Medical Research Institute, Gil Hospital, Incheon 21565, Korea. 
      hsjun@gachon.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20180524
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT3 Transcription Factor)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/*pharmacology
MH  - Humans
MH  - Interleukin-6/*pharmacology
MH  - Mice
MH  - Muscle Development/*drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - STAT3 Transcription Factor/drug effects/*metabolism
MH  - Stem Cells/*metabolism
PMC - PMC6032255
OTO - NOTNLM
OT  - ADSCs
OT  - IL-6
OT  - STAT3
OT  - muscle cell conditioned media
OT  - myogenic differentiation
COIS- The authors declare no conflict of interest.
EDAT- 2018/06/09 06:00
MHDA- 2018/10/16 06:00
PMCR- 2018/06/01
CRDT- 2018/06/09 06:00
PHST- 2018/04/27 00:00 [received]
PHST- 2018/05/20 00:00 [revised]
PHST- 2018/05/22 00:00 [accepted]
PHST- 2018/06/09 06:00 [entrez]
PHST- 2018/06/09 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PHST- 2018/06/01 00:00 [pmc-release]
AID - ijms19061557 [pii]
AID - ijms-19-01557 [pii]
AID - 10.3390/ijms19061557 [doi]
PST - epublish
SO  - Int J Mol Sci. 2018 May 24;19(6):1557. doi: 10.3390/ijms19061557.