PMID- 29884339
OWN - NLM
STAT- MEDLINE
DCOM- 20190201
LR  - 20190201
IS  - 1878-3554 (Electronic)
IS  - 0099-2399 (Linking)
VI  - 44
IP  - 7
DP  - 2018 Jul
TI  - Poly(Adenosine Phosphate Ribose) Polymerase 1 Inhibition Enhances Brain-derived 
      Neurotrophic Factor Secretion in Dental Pulp Stem Cell-derived Odontoblastlike 
      Cells.
PG  - 1121-1125
LID - S0099-2399(18)30236-X [pii]
LID - 10.1016/j.joen.2018.03.015 [doi]
AB  - INTRODUCTION: The nuclear enzyme poly(adenosine phosphate ribose) polymerase 1 
      (PARP-1) has been implicated in the maintenance and differentiation of several 
      stem cells. The role of PARP-1 in dental pulp stem cell (DPSC) differentiation, 
      especially in the context of its ability to modulate nerve regeneration factors, 
      has not been investigated. Regeneration of neuronal components in pulp tissue is 
      important for the assessment of tooth vitality. Brain-derived neurotrophic factor 
      (BDNF) is known to play an integral signaling factor during nerve regeneration. 
      In this study, we identified the role of PARP-1 in the modulation of BDNF in DPSC 
      differentiation into odontoblastlike cells. METHODS: Human DPSCs were prepared 
      from healthy molars and cultured in regular and osteogenic media treated with 
      PARP-1 antagonist and PARP-1 exogeneous protein. Polymerase chain reaction and 
      immunohistochemistry analysis for BDNF and various differentiation markers were 
      performed. RESULTS: Our polymerase chain reaction results showed that 
      differentiated cells show odontoblastlike properties because they express 
      odontogenic markers such as dentin sialophosphoprotein and dentin matrix protein 
      1. Both PARP-1 inhibitor and protein did not affect odontogenic differentiation 
      and proliferation because the number of the differentiated cells was unaffected, 
      and the expression of dentin sialophosphoprotein and dentin matrix protein 1 was 
      not significantly changed. There is the possibility that PARP-1 treatment induces 
      DPSCs into the unique cell lineage. Some differentiated cells show a very unique 
      morphology with large irregular cytoplasm and an oval nucleus. Moreover, PARP-1 
      inhibition significantly increased BDNF secretion in DPSC-derived odontoblastlike 
      cells. This observation was also confirmed by immunohistochemistry. CONCLUSIONS: 
      Taken together, our results indicate PARP-1 as a negative regulator in BDNF 
      secretion during odontogenic DPSC differentiation, showing its potential 
      application for translational nerve regeneration strategies to improve dental 
      pulp tissue vitality assessments.
CI  - Copyright (c) 2018 American Association of Endodontists. Published by Elsevier Inc. 
      All rights reserved.
FAU - Valverde, Yessenia
AU  - Valverde Y
AD  - Department of Oral Biology, University of Illinois at Chicago, Chicago, Illinois.
FAU - Narayanan, Raghuvaran
AU  - Narayanan R
AD  - Department of Endodontics, University of Illinois at Chicago, Chicago, Illinois.
FAU - Alapati, Satish B
AU  - Alapati SB
AD  - Department of Endodontics, University of Illinois at Chicago, Chicago, Illinois.
FAU - Chmilewsky, Fanny
AU  - Chmilewsky F
AD  - Department of Oral Biology, University of Illinois at Chicago, Chicago, Illinois.
FAU - Huang, Chun-Chieh
AU  - Huang CC
AD  - Department of Oral Biology, University of Illinois at Chicago, Chicago, Illinois.
FAU - Ravindran, Sriram
AU  - Ravindran S
AD  - Department of Oral Biology, University of Illinois at Chicago, Chicago, Illinois.
FAU - Chung, Seung H
AU  - Chung SH
AD  - Department of Oral Biology, University of Illinois at Chicago, Chicago, Illinois. 
      Electronic address: chungsh@uic.edu.
LA  - eng
PT  - Journal Article
DEP - 20180605
PL  - United States
TA  - J Endod
JT  - Journal of endodontics
JID - 7511484
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.4.2.30 (Poly (ADP-Ribose) Polymerase-1)
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Differentiation
MH  - Dental Pulp/*cytology/drug effects/metabolism
MH  - Humans
MH  - Nerve Regeneration
MH  - Odontoblasts/*cytology/drug effects/metabolism
MH  - Odontogenesis
MH  - Poly (ADP-Ribose) Polymerase-1/antagonists & inhibitors/*metabolism/physiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Regenerative Endodontics/methods
MH  - Stem Cells/drug effects/*metabolism
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - dental pulp stem cell
OT  - odontoblast
OT  - poly(adenosine phosphate ribose) polymerase 1
EDAT- 2018/06/10 06:00
MHDA- 2019/02/02 06:00
CRDT- 2018/06/10 06:00
PHST- 2017/12/21 00:00 [received]
PHST- 2018/02/23 00:00 [revised]
PHST- 2018/03/26 00:00 [accepted]
PHST- 2018/06/10 06:00 [pubmed]
PHST- 2019/02/02 06:00 [medline]
PHST- 2018/06/10 06:00 [entrez]
AID - S0099-2399(18)30236-X [pii]
AID - 10.1016/j.joen.2018.03.015 [doi]
PST - ppublish
SO  - J Endod. 2018 Jul;44(7):1121-1125. doi: 10.1016/j.joen.2018.03.015. Epub 2018 Jun 
      5.