PMID- 29886462
OWN - NLM
STAT- MEDLINE
DCOM- 20181106
LR  - 20181106
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 43
IP  - 5
DP  - 2018 May 28
TI  - [Effects of Pim-1 inhibitor on mouse model of inflammatory bowel disease induced 
      by TNBS].
PG  - 481-489
LID - 10.11817/j.issn.1672-7347.2018.05.004 [doi]
AB  - To explore the role of Pim-1 in the pathology of inflammatory bowel disease and 
      the potential effect of Pim-1 inhibitor on treating such disease.
 Methods: 
      Forty-five BALB/c mice were randomly divided into 5 groups (n=9): A normal 
      control group, a inflammatory bowel disease group, two different dose of Pim-1 
      inhibitor treatment groups, and steroidhormone treatment group. The model of 
      inflammatory bowel disease was induced by intracolonic administration of 2, 4, 
      6-trinitrobenzenestdfonic acid (TNBS) and ethanol mixture. Mice were treated with 
      Pim-1 inhibitor [intraperitoneal inject, 5 or 10 mg/(kg.d)] for 5 days and 
      prednisone (intragastric administration, 0.1 mg/d) for 5 days. The DAI, colon 
      length, gross score and pathological grade were evaluated. The expressions of T 
      cell master transcription factors T-box expressed in T cells (T-bet), GATA 
      binding protein 3 (GATA-3), RA orphan receptorgamma (RORgammat) and forkhead box P3 
      (Foxp3) were measured by Real-time PCR and Western blot, respectively.
 Results: 
      Pim-1 inhibitor and prednisone showed therapeutic effect on acute TNBS colitis in 
      vivo. GATA3 and RORgammat were significantly up-regulated in acute TNBS colitis 
      (P<0.05). In contrast, the expression of Foxp3 was suppressed in the inflammatory 
      bowel disease group, whereas it did not cause any significant change in T-bet 
      expression (P>0.05). Administration of Pim-1 inhibitor and prednisone resulted in 
      suppression of GATA3, RORgammat expression, and the increase of Foxp3 expression 
      (P<0.05). Administration of Pim-1 inhibitor and prednisone resulted in inhibition 
      of T-bet mRNA expression (P<0.05), but only prednisone could inhibit T-bet 
      protein expression (P>0.05).
 Conclusion: Pim-1 inhibitor significantly 
      suppresses Th2- and Th17-type immune responses. Furthermore, Pim-1 inhibitor 
      could induce T-cell differentiation towards a Treg phenotype. Pim-1 inhibitor has 
      therapeutic effect on acute TNBS colitis.
FAU - Ou, Rong
AU  - Ou R
AD  - Department of Gastroenterology, Huai'an Rehabilitation Hospital, Huai'an Jiangsu 
      211600, China.
FAU - Shen, Yueming
AU  - Shen Y
AD  - Department of Gastroenterology, 
Changsha Central Hospital, Changsha 410004, 
      China.
FAU - Zeng, Ya
AU  - Zeng Y
AD  - Department of Gastroenterology, 
Changsha Central Hospital, Changsha 410004, 
      China.
FAU - Zou, Lingzhi
AU  - Zou L
AD  - Department of Gastroenterology, Xiangya Hospital, Central South University, 
      
Changsha 410008, China.
FAU - Jiang, Na
AU  - Jiang N
AD  - Department of Gastroenterology, Xiangya Hospital, Central South University, 
      
Changsha 410008, China.
FAU - Xu, Meihua
AU  - Xu M
AD  - Department of Gastroenterology, Xiangya Hospital, Central South University, 
      
Changsha 410008, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Gata3 protein, mouse)
RN  - 0 (Nuclear Receptor Subfamily 1, Group F, Member 3)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 3K9958V90M (Ethanol)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
RN  - 9PHQ9Y1OLM (Prednisolone)
RN  - EC 2.7.11.1 (Pim1 protein, mouse)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-pim-1)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cell Differentiation
MH  - Colitis/chemically induced/*drug therapy/pathology
MH  - Colon/drug effects/pathology
MH  - Ethanol
MH  - Forkhead Transcription Factors/metabolism
MH  - GATA3 Transcription Factor/metabolism
MH  - Inflammatory Bowel Diseases/chemically induced/*drug therapy/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nuclear Receptor Subfamily 1, Group F, Member 3/metabolism
MH  - Prednisolone/pharmacology
MH  - Proto-Oncogene Proteins c-pim-1/*antagonists & inhibitors
MH  - Random Allocation
MH  - T-Box Domain Proteins/metabolism
MH  - T-Lymphocytes, Regulatory/cytology
MH  - Trinitrobenzenesulfonic Acid
EDAT- 2018/06/11 06:00
MHDA- 2018/11/07 06:00
CRDT- 2018/06/11 06:00
PHST- 2018/06/11 06:00 [entrez]
PHST- 2018/06/11 06:00 [pubmed]
PHST- 2018/11/07 06:00 [medline]
AID - 10.11817/j.issn.1672-7347.2018.05.004 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2018 May 28;43(5):481-489. doi: 
      10.11817/j.issn.1672-7347.2018.05.004.