PMID- 29888565
OWN - NLM
STAT- MEDLINE
DCOM- 20190405
LR  - 20190405
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 43
IP  - 5
DP  - 2018 May 25
TI  - [Effect of Electroacupuncture Preconditioning on Myocardial Ischemia and 
      Expression of TLR 4, MyD 88 and NF-kappaB mRNAs in "Neiguan" (PC 6) Area in Rats with 
      Myocardial Ischemia-reperfusion Injury].
PG  - 302-6
LID - 10.13702/j.1000-0607.170308 [doi]
AB  - OBJECTIVE: To observe the effect of electroacupuncture (EA) preconditioning on 
      acute myocardial ischemia (MI) and expression of Toll-like receptor 4 (TLR 4), 
      myeloid differentiation factor 88 (MyD 88) and nuclear factor-kappaB (NF-kappaB) mRNAs in 
      the "Neiguan" (PC 6) area of rats with MI-reperfusion injury (MIRI), so as to 
      explore its mechanisms underlying improvement of MIRI. METHODS: Forty-eight male 
      Wistar rats were randomly divided into control, control ＋ EA, sham operation, 
      model, EA and sham EA groups (n＝8). The MIRI model was established by occlusion 
      of the descending anterior branch of the left coronary artery for 30 min, 
      followed by reperfusion. Before modeling, EA preconditioning was respectively 
      applied to PC 6 or sham PC 6 for 30 min, once daily for 5 days. Electrocardiogram 
      (ECG) of the standard limb-lead Ⅱ was recorded before and after the modeling, and 
      0.25 h after reperfusion. The expression levels of TLR 4, MyD 88 and NF-kappaB genes 
      in the PC 6 tissue were detected by Real-time PCR. RESULTS: Compared with the 
      control group, the height of ECG J-point was significantly increased in the model 
      group (P<0.01), suggesting an acute MI, and the height of J-point was 
      significantly lower in the EA group than in the model and sham EA groups 
      (P<0.01). In comparison with the control group, the expression levels of TLR 4, 
      MyD 88 and NF-kappaB mRNAs were markedly up-regulated in the area of PC 6 in the 
      model group (P<0.01). Compared with the model group, the increased expression 
      levels of TLR 4, MyD 88 and NF-kappaB mRNAs in the EA preconditioning group were 
      significantly inhibited (P<0.05). No significant differences were found among the 
      control, control＋EA and sham operation groups and between the model and sham EA 
      groups in the expression levels of TLR 4, MyD 88 and NF-kappaB mRNAs (P>0.05)．. 
      CONCLUSION: EA preconditioning can improve myocardial ischemia and reduce the 
      expression of TLR 4, MyD 88 and NF-kappaB mRNAs in PC 6 area of MIRI rats, suggesting 
      an involvement of the regional cutaneous TLR 4/MyD 88/NF-kappaB signaling suppression 
      in the initiation-transfer-amplification of EA stimulating signal inputs for 
      relieving MIRI.
FAU - Zhu, Yi
AU  - Zhu Y
AD  - Department of Acupuncture-moxibustion, Yueyang Hospital of Integrated Traditional 
      Chinese and Western Medicine, Shanghai University of Traditional Chinese 
      Medicine, Shanghai 200437, China.
FAU - Li, Yi
AU  - Li Y
AD  - Department of Acupuncture-moxibustion, Yueyang Hospital of Integrated Traditional 
      Chinese and Western Medicine, Shanghai University of Traditional Chinese 
      Medicine, Shanghai 200437, China.
FAU - Zhang, Nan
AU  - Zhang N
AD  - Department of Acupuncture-moxibustion, Yueyang Hospital of Integrated Traditional 
      Chinese and Western Medicine, Shanghai University of Traditional Chinese 
      Medicine, Shanghai 200437, China.
FAU - Dong, Gui-Rong
AU  - Dong GR
AD  - Department of Acupuncture-moxibustion, Yueyang Hospital of Integrated Traditional 
      Chinese and Western Medicine, Shanghai University of Traditional Chinese 
      Medicine, Shanghai 200437, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Acupuncture Points
MH  - Animals
MH  - *Electroacupuncture
MH  - Male
MH  - *Myocardial Ischemia
MH  - NF-kappa B
MH  - Rats
MH  - Rats, Wistar
MH  - *Reperfusion Injury
OTO - NOTNLM
OT  - Cutaneous TLR 4/MyD 88/NF-kappaB signaling
OT  - Electroacupuncture
OT  - Myocardial ischemia-reperfusion injury
OT  - PC 6 area
EDAT- 2018/06/12 06:00
MHDA- 2019/04/06 06:00
CRDT- 2018/06/12 06:00
PHST- 2018/06/12 06:00 [entrez]
PHST- 2018/06/12 06:00 [pubmed]
PHST- 2019/04/06 06:00 [medline]
AID - 10.13702/j.1000-0607.170308 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2018 May 25;43(5):302-6. doi: 10.13702/j.1000-0607.170308.