PMID- 29888657 OWN - NLM STAT- MEDLINE DCOM- 20181114 LR - 20181114 IS - 1876-2891 (Electronic) IS - 1876-2883 (Linking) VI - 9 IP - 5 DP - 2018 Sep 18 TI - Oral administration of Bifidobacterium bifidum TMC3115 to neonatal mice may alleviate IgE-mediated allergic risk in adulthood. PG - 815-828 LID - 10.3920/BM2018.0005 [doi] AB - This study aimed to demonstrate whether exposure to bifidobacteria during early life influences immunity and alleviates the risk of immunoglobulin E (IgE)-mediated allergies in adulthood. BALB/c neonatal mice (n=54) were administered with a lyophilised cell preparation of Bifidobacterium bifidum TMC3115 (TMC3115) for 3 weeks. Following the intervention, the mice were immunised with intraperitoneal ovalbumin (OVA). The morphology and function of the intestinal epithelium were determined using histopathological examinations. Intestinal microbiota was detected using quantitative PCR and characterised using next-generation sequencing of 16S rRNA genes from faecal DNA. Caecal short-chain fatty acids (SCFAs) were measured using gas chromatography-mass spectrometry. Serum levels of tumour necrosis factor (TNF)-alpha, interleukin (IL)-6, IL-10, and immunoglobulin E (IgE) and the percentage of splenic CD4+ T cells were examined using enzyme-linked immunosorbent assay and flow cytometry, respectively. TMC3115 did not significantly affect body weight, and cause any severe systemic inflammation or other clinical symptoms among the neonatal or adult mice, although the crypt depths and Muc2-positive cells in some intestinal segments of neonatal mice were significantly lower than control. Oral TMC3115 administration significantly increased faecal microbial diversity, relative abundance of Bacteroidetes and caecal SCFAs production in neonatal mice. Following the intervention, neonatal mice treated with TMC3115 exhibited less increase in serum IgE levels induced by OVA in adults and significantly higher TNF-alpha and IL-10 levels than in control. Our findings indicate that the oral administration of bifidobacteria, particularly certain strains, such as TMC3115, during early life could alleviate the risk of IgE-mediated allergies in adult host animals. Modifications of intestinal microbiota, SCFAs metabolism and anti-inflammatory cytokine IL-10 production by bifidobacteria may at least in part be a key mechanism underlying the effect of bifidobacteria on the IgE-mediated immune sensitivity of hosts to attacks by allergens at both neonatal and adult stages. FAU - Cheng, R Y AU - Cheng RY AD - 1 Department of Nutrition, Food Hygiene and Toxicology, West China School of Public Health, Sichuan University and Healthy Food Evaluation Research Center, Chengdu 610041, Sichuan, China P.R. FAU - Yao, J R AU - Yao JR AD - 2 West China Second University Hospital/Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Sichuan University, Chengdu 610041, Sichuan, China P.R. FAU - Wan, Q AU - Wan Q AD - 1 Department of Nutrition, Food Hygiene and Toxicology, West China School of Public Health, Sichuan University and Healthy Food Evaluation Research Center, Chengdu 610041, Sichuan, China P.R. FAU - Guo, J W AU - Guo JW AD - 1 Department of Nutrition, Food Hygiene and Toxicology, West China School of Public Health, Sichuan University and Healthy Food Evaluation Research Center, Chengdu 610041, Sichuan, China P.R. FAU - Pu, F F AU - Pu FF AD - 1 Department of Nutrition, Food Hygiene and Toxicology, West China School of Public Health, Sichuan University and Healthy Food Evaluation Research Center, Chengdu 610041, Sichuan, China P.R. FAU - Shi, L AU - Shi L AD - 3 Department of Clinical Nutrition, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China P.R. FAU - Hu, W AU - Hu W AD - 3 Department of Clinical Nutrition, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China P.R. FAU - Yang, Y H AU - Yang YH AD - 4 Department of Planning, Hebei Inatural Biotech Co., Ltd. Shijiazhuang 050000, Hebei, China P.R. FAU - Li, L AU - Li L AD - 5 Department of Research and Development, Hebei Inatural Biotech Co., Ltd. Shijiazhuang 050000, Hebei, China P.R. FAU - Li, M AU - Li M AD - 1 Department of Nutrition, Food Hygiene and Toxicology, West China School of Public Health, Sichuan University and Healthy Food Evaluation Research Center, Chengdu 610041, Sichuan, China P.R. FAU - He, F AU - He F AD - 1 Department of Nutrition, Food Hygiene and Toxicology, West China School of Public Health, Sichuan University and Healthy Food Evaluation Research Center, Chengdu 610041, Sichuan, China P.R. LA - eng PT - Journal Article DEP - 20180611 PL - Netherlands TA - Benef Microbes JT - Beneficial microbes JID - 101507616 RN - 0 (Interleukin-6) RN - 0 (Tumor Necrosis Factor-alpha) RN - 130068-27-8 (Interleukin-10) RN - 37341-29-0 (Immunoglobulin E) RN - 9006-59-1 (Ovalbumin) SB - IM MH - Administration, Oral MH - Animals MH - Animals, Newborn/immunology MH - Bifidobacterium bifidum/*physiology MH - Enzyme-Linked Immunosorbent Assay MH - Female MH - Gastrointestinal Microbiome MH - Humans MH - Hypersensitivity/*drug therapy/etiology/immunology/microbiology MH - Immunoglobulin E/*immunology MH - Infant, Newborn MH - Infant, Newborn, Diseases/drug therapy/genetics/immunology/microbiology MH - Interleukin-10/genetics/immunology MH - Interleukin-6/genetics/immunology MH - Intestines/immunology/microbiology MH - Male MH - Mice MH - Mice, Inbred BALB C MH - Ovalbumin/adverse effects/immunology MH - Probiotics/*administration & dosage MH - Tumor Necrosis Factor-alpha/genetics/immunology OTO - NOTNLM OT - allergy OT - bifidobacteria OT - early life OT - immunity OT - intestinal microbiota EDAT- 2018/06/12 06:00 MHDA- 2018/11/15 06:00 CRDT- 2018/06/12 06:00 PHST- 2018/06/12 06:00 [pubmed] PHST- 2018/11/15 06:00 [medline] PHST- 2018/06/12 06:00 [entrez] AID - 10.3920/BM2018.0005 [doi] PST - ppublish SO - Benef Microbes. 2018 Sep 18;9(5):815-828. doi: 10.3920/BM2018.0005. Epub 2018 Jun 11.