PMID- 29888818
OWN - NLM
STAT- MEDLINE
DCOM- 20190603
LR  - 20190603
IS  - 1521-3765 (Electronic)
IS  - 0947-6539 (Linking)
VI  - 24
IP  - 44
DP  - 2018 Aug 6
TI  - Exfoliated Nanosheets of a Cu(II) Coordination Polymer Modulate Enzyme Activity 
      of alpha-Chymotrypsin.
PG  - 11297-11302
LID - 10.1002/chem.201802376 [doi]
AB  - A 2D coordination polymer derived from 5-azidoisophthalic acid (AIA) and Cu(NO(3) 
      )(2) was designed with the aim of modulating the activity of a digestive enzyme 
      alpha-chymotrypsin (ChT). The coordination polymer namely [Cu(0.5) (mu-AIA)(0.5) 
      (H(2) O)]⋅2 H(2) O(alpha) (CP1) was successfully synthesized and fully characterized 
      by single-crystal X-ray diffraction (SXRD). An exfoliated nanosheet (ENS) of CP1 
      was readily produced by overnight stirring of hand-ground CP1 crystals dispersed 
      in DMSO. ENS(CP1) was demonstrated to be acting as an inhibitor of ChT; as much 
      as  approximately 97 % inhibition of ChT was achieved with 100 mum of ENS(CP1) using 
      N-succinyl-l-phenylalanine-p-nitroanilide (SPNA) as substrate. Enzyme kinetics 
      data revealed that the inhibition of ChT followed a competitive pathway. An 
      enzyme assay under varying ionic strength and varying concentration of free 
      histidine revealed that the active site His-57 participated in coordination with 
      the Cu(II) metal center of ENS(CP1) thereby preventing the substrate (SPNA) from 
      binding with the enzyme resulting in efficient inhibition.
CI  - (c) 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Sarkar, Koushik
AU  - Sarkar K
AD  - Department of Organic Chemistry, Indian Association for the Cultivation of 
      Science (IACS), 2A and 2B, Raja S. C. Mullick Road, Jadavpur, Kolkata, 700032, 
      West Bengal, India.
FAU - Dastidar, Parthasarathi
AU  - Dastidar P
AUID- ORCID: 0000-0002-1011-2386
AD  - Department of Organic Chemistry, Indian Association for the Cultivation of 
      Science (IACS), 2A and 2B, Raja S. C. Mullick Road, Jadavpur, Kolkata, 700032, 
      West Bengal, India.
LA  - eng
PT  - Journal Article
DEP - 20180710
PL  - Germany
TA  - Chemistry
JT  - Chemistry (Weinheim an der Bergstrasse, Germany)
JID - 9513783
RN  - 0 (Coordination Complexes)
RN  - 0 (Phthalic Acids)
RN  - 0 (Polymers)
RN  - 2440-62-2 (Suphepa)
RN  - 3600-77-9 (5-azidoisophthalic acid)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - 4QD397987E (Histidine)
RN  - 789U1901C5 (Copper)
RN  - EC 3.4.21.1 (Chymotrypsin)
RN  - EC 3.4.21.1 (alpha-chymotrypsin)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Catalysis
MH  - Cell Survival/drug effects
MH  - Chymotrypsin/*antagonists & inhibitors/chemistry
MH  - Coordination Complexes/*chemical synthesis/chemistry/pharmacology
MH  - Copper/*chemistry
MH  - Histidine/chemistry
MH  - Kinetics
MH  - Mice
MH  - Models, Molecular
MH  - Nanostructures/*chemistry
MH  - Osmolar Concentration
MH  - Phenylalanine/analogs & derivatives/chemistry
MH  - Phthalic Acids/*chemistry
MH  - Polymers/*chemical synthesis/chemistry/pharmacology
MH  - Protein Conformation
MH  - RAW 264.7 Cells
OTO - NOTNLM
OT  - biocompatibility
OT  - coordination polymer
OT  - enzymatic modulation
OT  - exfoliation
OT  - alpha-chymotrypsin
EDAT- 2018/06/12 06:00
MHDA- 2019/06/04 06:00
CRDT- 2018/06/12 06:00
PHST- 2018/05/12 00:00 [received]
PHST- 2018/06/06 00:00 [revised]
PHST- 2018/06/12 06:00 [pubmed]
PHST- 2019/06/04 06:00 [medline]
PHST- 2018/06/12 06:00 [entrez]
AID - 10.1002/chem.201802376 [doi]
PST - ppublish
SO  - Chemistry. 2018 Aug 6;24(44):11297-11302. doi: 10.1002/chem.201802376. Epub 2018 
      Jul 10.