PMID- 29890625
OWN - NLM
STAT- MEDLINE
DCOM- 20181101
LR  - 20181114
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 10
IP  - 6
DP  - 2018 Jun 8
TI  - Hepatic Mitochondrial Dysfunction and Immune Response in a Murine Model of Peanut 
      Allergy.
LID - 10.3390/nu10060744 [doi]
LID - 744
AB  - BACKGROUND: Evidence suggests a relevant role for liver and mitochondrial 
      dysfunction in allergic disease. However, the role of hepatic mitochondrial 
      function in food allergy is largely unknown. We aimed to investigate hepatic 
      mitochondrial dysfunction in a murine model of peanut allergy. METHODS: 
      Three-week-old C3H/HeOuJ mice were sensitized by the oral route with 
      peanut-extract (PNT). We investigated: 1. the occurrence of effective 
      sensitization to PNT by analysing acute allergic skin response, anaphylactic 
      symptoms score, body temperature, serum mucosal mast cell protease-1 (mMCP-1) and 
      anti-PNT immunoglobulin E (IgE) levels; 2. hepatic involvement by analysing 
      interleukin (IL)-4, IL-5, IL-13, IL-10 and IFN-γ mRNA expression; 3. 
      hepatic mitochondrial oxidation rates and efficiency by polarography, and 
      hydrogen peroxide (H(2)O(2)) yield, aconitase and superoxide dysmutase activities by 
      spectrophotometry. RESULTS: Sensitization to PNT was demonstrated by acute 
      allergic skin response, anaphylactic symptoms score, body temperature decrease, 
      serum mMCP-1 and anti-peanut IgE levels. Liver involvement was demonstrated by a 
      significant increase of hepatic Th2 cytokines (IL-4, IL-5 and IL-13) mRNA 
      expression. Mitochondrial dysfunction was demonstrated by lower state 3 
      respiration rate in the presence of succinate, decreased fatty acid oxidation in 
      the presence of palmitoyl-carnitine, increased yield of ROS proven by the 
      inactivation of aconitase enzyme and higher H(2)O(2) mitochondrial release. 
      CONCLUSIONS: We provide evidence of hepatic mitochondrial dysfunction in a murine 
      model of peanut allergy. These data could open the way to the identification of 
      new mitochondrial targets for innovative preventive and therapeutic strategies 
      against food allergy.
FAU - Trinchese, Giovanna
AU  - Trinchese G
AD  - Department of Biology, University of Naples "Federico II", 80126 Naples, Italy. 
      giovanna.trinchese@unina.it.
FAU - Paparo, Lorella
AU  - Paparo L
AD  - Department of Translational Medical Science-Pediatric Section, University of 
      Naples "Federico II", 80131 Naples, Italy. paparolorella@gmail.com.
FAU - Aitoro, Rosita
AU  - Aitoro R
AD  - Department of Translational Medical Science-Pediatric Section, University of 
      Naples "Federico II", 80131 Naples, Italy. aitoro.rosita@gmail.com.
FAU - Fierro, Carmela
AU  - Fierro C
AD  - Department of Translational Medical Science-Pediatric Section, University of 
      Naples "Federico II", 80131 Naples, Italy. carmelafierro0@gmail.com.
FAU - Varchetta, Michela
AU  - Varchetta M
AD  - Department of Translational Medical Science-Pediatric Section, University of 
      Naples "Federico II", 80131 Naples, Italy. mic.varchetta@studenti.unina.it.
FAU - Nocerino, Rita
AU  - Nocerino R
AD  - Department of Translational Medical Science-Pediatric Section, University of 
      Naples "Federico II", 80131 Naples, Italy. ritanocerino@alice.it.
FAU - Mollica, Maria Pina
AU  - Mollica MP
AD  - Department of Biology, University of Naples "Federico II", 80126 Naples, Italy. 
      mariapia.mollica@unina.it.
FAU - Berni Canani, Roberto
AU  - Berni Canani R
AUID- ORCID: 0000-0002-5169-9574
AD  - Department of Translational Medical Science-Pediatric Section, University of 
      Naples "Federico II", 80131 Naples, Italy. berni@unina.it.
AD  - European Laboratory for the Investigation of Food-Induced Diseases (ELFID), 
      University of Naples "Federico II", 80131 Naples, Italy. berni@unina.it.
AD  - CEINGE Advanced Biotechnologies, University of Naples "Federico II", 80131 
      Naples, Italy. berni@unina.it.
AD  - Task Force on Microbiome Studies, University of Naples "Federico II", 80131 
      Naples, Italy. berni@unina.it.
LA  - eng
PT  - Journal Article
DEP - 20180608
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Allergens)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-5)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Arachis/*immunology
MH  - Disease Models, Animal
MH  - *Energy Metabolism
MH  - Female
MH  - Immunoglobulin E/blood
MH  - Interleukin-13/genetics/immunology/metabolism
MH  - Interleukin-4/genetics/immunology/metabolism
MH  - Interleukin-5/genetics/immunology/metabolism
MH  - Liver/*immunology/metabolism
MH  - Mice, Inbred C3H
MH  - Mitochondria, Liver/*immunology/metabolism
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - Peanut Hypersensitivity/genetics/*immunology/metabolism
MH  - Plant Proteins/*immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Th2 Cells/*immunology/metabolism
MH  - Up-Regulation
PMC - PMC6024519
OTO - NOTNLM
OT  - Th2 cytokines
OT  - food allergy
OT  - mitochondrial function
OT  - oxidative stress
COIS- The authors declare no conflict of interest.
EDAT- 2018/06/13 06:00
MHDA- 2018/11/02 06:00
PMCR- 2018/06/01
CRDT- 2018/06/13 06:00
PHST- 2018/04/27 00:00 [received]
PHST- 2018/06/04 00:00 [revised]
PHST- 2018/06/06 00:00 [accepted]
PHST- 2018/06/13 06:00 [entrez]
PHST- 2018/06/13 06:00 [pubmed]
PHST- 2018/11/02 06:00 [medline]
PHST- 2018/06/01 00:00 [pmc-release]
AID - nu10060744 [pii]
AID - nutrients-10-00744 [pii]
AID - 10.3390/nu10060744 [doi]
PST - epublish
SO  - Nutrients. 2018 Jun 8;10(6):744. doi: 10.3390/nu10060744.