PMID- 29892291
OWN - NLM
STAT- MEDLINE
DCOM- 20190730
LR  - 20190730
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 9
DP  - 2018
TI  - Immunomodulation of Host Chitinase 3-Like 1 During a Mammary Pathogenic 
      Escherichia coli Infection.
PG  - 1143
LID - 10.3389/fimmu.2018.01143 [doi]
LID - 1143
AB  - Chitin is a N-acetyl-d-glucosamine biopolymer that can be recognized by 
      chitin-binding proteins. Although mammals lack chitin synthase, they induce 
      proteins responsible for detecting chitin in response to bacterial infections. 
      Our aim was to investigate whether chitinase 3-like 1 (CHI3L1) has a potential 
      role in the innate immunity of the Escherichia coli (E. coli) infected mammary 
      gland. CHI3L1 protein was found to be secreted in whey of naturally 
      coliform-affected quarters compared to whey samples isolated from healthy udders. 
      In addition, gene expression of CHI3L1 was confirmed in udder tissue of cows 
      experimentally infected with a mammary pathogenic E. coli (MPEC) strain. Despite 
      the known anatomical differences, the bovine udders' innate immune response was 
      mimicked by applying an experimental mouse model using MPEC or non-MPEC isolates. 
      The effect of CHI3L1 expression in the murine mammary gland in response to 
      coliform bacteria was investigated through the use of CHI3L1(-/-) mice as well as 
      through treatment with either a pan-caspase inhibitor or chitin particles in 
      wild-type mice. The local induction of CHI3L1 postinfection with different E. 
      coli strains was demonstrated to be independent of both bacterial growth and 
      mammary interleukin (IL)-8 levels. Indeed, CHI3L1 emerged as a regulator 
      impacting on the transcytosis of Ly6G-positive cells from the interstitial space 
      into the alveolar lumen of the mammary tissue. Furthermore, CHI3L1 was found to 
      be upstream regulated by caspase activity and had a major downstream effect on 
      the local pro-inflammatory cytokine profile, including IL-1beta, IL-6, and 
      RANTES/CCL5. In conclusion, CHI3L1 was demonstrated to play a key role in the 
      cytokine and caspase signaling during E. coli triggered inflammation of the 
      mammary gland.
FAU - Breyne, Koen
AU  - Breyne K
AD  - Laboratory of Biochemistry, Department of Pharmacology, Toxicology and 
      Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, 
      Belgium.
FAU - Steenbrugge, Jonas
AU  - Steenbrugge J
AD  - Laboratory of Biochemistry, Department of Pharmacology, Toxicology and 
      Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, 
      Belgium.
FAU - Demeyere, Kristel
AU  - Demeyere K
AD  - Laboratory of Biochemistry, Department of Pharmacology, Toxicology and 
      Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, 
      Belgium.
FAU - Lee, Chun Geun
AU  - Lee CG
AD  - Division of Biology and Medicine, Warren Alpert School of Medicine at Brown 
      University, Providence, RI, United States.
FAU - Elias, Jack A
AU  - Elias JA
AD  - Division of Biology and Medicine, Warren Alpert School of Medicine at Brown 
      University, Providence, RI, United States.
FAU - Petzl, Wolfram
AU  - Petzl W
AD  - Clinic for Ruminants with Ambulance and Herd Health Services, Centre for Clinical 
      Veterinary Medicine, Ludwig-Maximilians-University of Munich, Munich, Germany.
FAU - Smith, David G E
AU  - Smith DGE
AD  - Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt 
      University, Edinburgh, United Kingdom.
FAU - Germon, Pierre
AU  - Germon P
AD  - INRA UMR 1282 Infectiologie et Sante Publique (ISP), Universite Francois Rabelais 
      de Tours, Nouzilly, France.
FAU - Meyer, Evelyne
AU  - Meyer E
AD  - Laboratory of Biochemistry, Department of Pharmacology, Toxicology and 
      Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, 
      Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180528
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Chitinase-3-Like Protein 1)
RN  - 0 (Cytokines)
RN  - 1398-61-4 (Chitin)
RN  - EC 3.4.22.- (Caspases)
MH  - Animals
MH  - Bacterial Load
MH  - Caspases/metabolism
MH  - Cattle
MH  - Chitin/metabolism
MH  - Chitinase-3-Like Protein 1/genetics/*metabolism
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Escherichia coli/*immunology
MH  - Escherichia coli Infections/genetics/*immunology/microbiology
MH  - Female
MH  - Gene Expression
MH  - *Immunomodulation/genetics
MH  - Mastitis, Bovine/genetics/*immunology/*microbiology/pathology
MH  - Mice
MH  - Mice, Knockout
PMC - PMC5985307
OTO - NOTNLM
OT  - Escherichia coli
OT  - bovine mastitis
OT  - chitinase 3-like 1
OT  - immunomodulation
OT  - mouse mammary gland infection model
EDAT- 2018/06/13 06:00
MHDA- 2018/06/13 06:01
PMCR- 2018/01/01
CRDT- 2018/06/13 06:00
PHST- 2017/11/15 00:00 [received]
PHST- 2018/05/07 00:00 [accepted]
PHST- 2018/06/13 06:00 [entrez]
PHST- 2018/06/13 06:00 [pubmed]
PHST- 2018/06/13 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2018.01143 [doi]
PST - epublish
SO  - Front Immunol. 2018 May 28;9:1143. doi: 10.3389/fimmu.2018.01143. eCollection 
      2018.